Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   LIS75_RS11670 Genome accession   NZ_CP085388
Coordinates   2421629..2422429 (-) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus velezensis strain CMF18     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 2416629..2427429
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIS75_RS11640 (LIS75_11650) - 2417179..2417811 (+) 633 WP_014418348.1 helix-turn-helix domain-containing protein -
  LIS75_RS11645 (LIS75_11655) - 2418102..2418239 (+) 138 WP_014470646.1 hypothetical protein -
  LIS75_RS11650 (LIS75_11660) - 2418333..2419451 (+) 1119 WP_015388019.1 NADH-dependent flavin oxidoreductase -
  LIS75_RS11655 (LIS75_11665) - 2419577..2419789 (+) 213 WP_003153183.1 spore germination protein -
  LIS75_RS11660 (LIS75_11670) - 2419915..2420127 (+) 213 WP_014305392.1 spore germination protein -
  LIS75_RS11665 (LIS75_11675) alr 2420302..2421483 (+) 1182 WP_015388018.1 alanine racemase -
  LIS75_RS11670 (LIS75_11680) spo0A 2421629..2422429 (-) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  LIS75_RS11675 (LIS75_11685) spoIVB 2422710..2423990 (-) 1281 WP_007408360.1 SpoIVB peptidase -
  LIS75_RS11680 (LIS75_11690) recN 2424153..2425883 (-) 1731 WP_003153174.1 DNA repair protein RecN Machinery gene
  LIS75_RS11685 (LIS75_11695) argR 2425915..2426364 (-) 450 WP_003153173.1 transcriptional regulator ArgR -
  LIS75_RS11690 (LIS75_11700) - 2426461..2427306 (-) 846 WP_015388017.1 TlyA family RNA methyltransferase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=617279 LIS75_RS11670 WP_003153177.1 2421629..2422429(-) (spo0A) [Bacillus velezensis strain CMF18]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=617279 LIS75_RS11670 WP_003153177.1 2421629..2422429(-) (spo0A) [Bacillus velezensis strain CMF18]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCCGATGTGCTCG
TGTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGTGAATCAGAGCTTGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCATCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCCC
CGTCTGCGCAAAGCAGCATTATCCGCAGCCAGCCGGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAACCAACCAACTCAGAGTTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97