Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   LI754_RS00180 Genome accession   NZ_CP085320
Coordinates   44302..45651 (-) Length   449 a.a.
NCBI ID   WP_044291476.1    Uniprot ID   -
Organism   Staphylococcus aureus strain CJFA-182     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 44302..92004 44302..45651 within 0


Gene organization within MGE regions


Location: 44302..92004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LI754_RS00180 ccrA 44302..45651 (-) 1350 WP_044291476.1 cassette chromosome recombinase CcrA Machinery gene
  LI754_RS00185 - 45842..46120 (-) 279 WP_044291478.1 hypothetical protein -
  LI754_RS00190 cch1 46190..47989 (-) 1800 WP_044291481.1 cassette chromosome replicative helicase -
  LI754_RS00195 - 48117..49544 (-) 1428 WP_044291483.1 DUF6038 family protein -
  LI754_RS00200 - 49661..50425 (+) 765 WP_044291485.1 DUF6414 family protein -
  LI754_RS00205 - 50415..51530 (+) 1116 WP_052502247.1 AAA family ATPase -
  LI754_RS00210 - 51676..52353 (+) 678 WP_044291487.1 hypothetical protein -
  LI754_RS00215 - 52601..52876 (+) 276 WP_044291488.1 hypothetical protein -
  LI754_RS00220 - 53221..54045 (-) 825 WP_044291491.1 alpha/beta fold hydrolase -
  LI754_RS00225 - 54138..54446 (+) 309 WP_228481663.1 hypothetical protein -
  LI754_RS00230 - 54577..55581 (-) 1005 WP_002511731.1 NADP-dependent oxidoreductase -
  LI754_RS00235 - 55771..56112 (+) 342 WP_002506307.1 MerR family transcriptional regulator -
  LI754_RS00240 - 56185..56544 (+) 360 WP_002511730.1 DUF1304 domain-containing protein -
  LI754_RS00245 - 56617..57381 (-) 765 WP_044291500.1 2OG-Fe dioxygenase family protein -
  LI754_RS00250 - 57880..58050 (+) 171 Protein_47 copper-transporting ATPase -
  LI754_RS00255 - 58068..58613 (+) 546 WP_044291506.1 YdhK family protein -
  LI754_RS00260 - 59081..59401 (+) 321 WP_232045967.1 hypothetical protein -
  LI754_RS00265 - 59586..60437 (+) 852 WP_044291507.1 LEM-3-like GIY-YIG domain-containing protein -
  LI754_RS00270 - 60467..62476 (+) 2010 WP_044291509.1 hypothetical protein -
  LI754_RS00275 - 62674..63783 (+) 1110 WP_044291510.1 DNA polymerase -
  LI754_RS00280 - 63776..64144 (+) 369 WP_037576907.1 hypothetical protein -
  LI754_RS00285 - 64144..65787 (+) 1644 WP_044291514.1 DUF5906 domain-containing protein -
  LI754_RS00290 ccrC 65974..67649 (+) 1676 Protein_55 cassette chromosome recombinase CcrC -
  LI754_RS00295 - 67755..68093 (+) 339 WP_044291525.1 SAUGI family uracil-DNA glycosylase inhibitor -
  LI754_RS00300 - 68189..68500 (+) 312 WP_044291529.1 DUF960 family protein -
  LI754_RS00305 - 68516..69022 (+) 507 WP_044291531.1 DUF1643 domain-containing protein -
  LI754_RS00310 - 69043..69489 (+) 447 WP_044291533.1 JAB domain-containing protein -
  LI754_RS00315 - 69659..70531 (+) 873 WP_044291536.1 WYL domain-containing protein -
  LI754_RS00320 - 70588..70878 (+) 291 WP_044291538.1 hypothetical protein -
  LI754_RS00325 - 70894..71388 (+) 495 WP_044291540.1 hypothetical protein -
  LI754_RS00330 - 71522..71659 (-) 138 WP_095231730.1 hypothetical protein -
  LI754_RS00335 - 71673..72557 (-) 885 WP_044291542.1 permease -
  LI754_RS00340 - 72647..72967 (-) 321 WP_040030671.1 ArsR/SmtB family transcription factor -
  LI754_RS00350 arsR 73405..73719 (+) 315 WP_040030669.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
  LI754_RS00355 arsB 73716..75008 (+) 1293 WP_181875449.1 arsenite efflux transporter membrane subunit ArsB -
  LI754_RS00360 arsC 75026..75427 (+) 402 WP_044291550.1 arsenate reductase (thioredoxin) -
  LI754_RS00365 - 75447..75788 (-) 342 WP_025907459.1 ArsR/SmtB family transcription factor -
  LI754_RS00370 - 75807..76424 (-) 618 WP_011274431.1 CadD family cadmium resistance transporter -
  LI754_RS00375 - 76978..78234 (+) 1257 WP_000566670.1 MrcB family domain-containing protein -
  LI754_RS00380 - 78649..78888 (+) 240 WP_001282728.1 hypothetical protein -
  LI754_RS00385 - 78920..79594 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  LI754_RS00390 - 79624..80121 (-) 498 WP_044291557.1 DnaJ domain-containing protein -
  LI754_RS00395 - 80774..80853 (+) 80 Protein_75 peptide-binding protein -
  LI754_RS00400 - 80902..80985 (+) 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
  LI754_RS00405 erm(B) 81110..81847 (+) 738 WP_001038795.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
  LI754_RS00410 - 82110..82490 (-) 381 Protein_78 toxin zeta -
  LI754_RS00415 - 82426..83001 (+) 576 WP_033603447.1 IS30 family transposase -
  LI754_RS00420 narB 83073..84686 (-) 1614 WP_002333004.1 ionophore ABC transporter permease subunit NarB -
  LI754_RS00425 narA 84710..85591 (-) 882 WP_002326774.1 ionophore ABC transporter ATP-binding subunit NarA -
  LI754_RS00430 - 85902..86498 (+) 597 WP_002326773.1 TetR/AcrR family transcriptional regulator -
  LI754_RS00435 - 86668..87342 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  LI754_RS00440 - 87378..87767 (+) 390 WP_020444595.1 hydroxymethylglutaryl-CoA synthase -
  LI754_RS00445 - 88684..89427 (+) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  LI754_RS00450 - 89524..89952 (+) 429 WP_000872606.1 MaoC family dehydratase -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52127.79 Da        Isoelectric Point: 9.9198

>NTDB_id=617178 LI754_RS00180 WP_044291476.1 44302..45651(-) (ccrA) [Staphylococcus aureus strain CJFA-182]
MKQAMGYLRQSTTKQQSLAAQKQTIEALAQKHNIPHITFYSDKQSGRTDKRNGYQQITELIQQGQCDVLCCYRLNRLHRN
LKNALKLMKLCQTYHVHILSVHDGYFDMDKAFDRLKLNIFISLAELESDNIGEQVKNGIKEKAKQGKLITTHAPFGYHYH
NGTFTIDTVKAPTVKAVFNYYLQGYGYKKIAQYLEADDKLINRKPYQVRNIILNPNYCGRVINQYGQYENMFPAIVSTTI
YEEAQVTRTQKQVKRKPSENQLKQKIKCPYCHSTLTNITIRKTDHSLRYYVCPQNMNNARFVCEFKGINAQELETSVLAT
CQDFFQNQQLYSKINHTIQQRLKRQRMHDTSNTLTQEKLIENLAQGKIDVETFREQSQSINLQNKPIQAISDIRIKASLQ
KVIQKSFTLNMLNPYIDEIRITKNKALVGIYFKNEPLNIVNQTSQSSIV

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=617178 LI754_RS00180 WP_044291476.1 44302..45651(-) (ccrA) [Staphylococcus aureus strain CJFA-182]
ATGAAACAAGCAATGGGTTACTTACGACAGAGCACTACAAAGCAACAATCCTTAGCAGCACAAAAACAAACCATCGAGGC
ATTAGCCCAAAAACATAATATTCCACATATTACTTTTTATAGTGATAAACAATCAGGACGTACTGATAAGCGGAATGGTT
ACCAACAAATTACTGAACTGATTCAACAAGGACAATGTGATGTATTATGTTGCTATCGTCTGAATCGACTTCATCGTAAT
CTAAAAAATGCATTAAAACTCATGAAATTGTGTCAAACGTATCATGTTCATATCTTAAGCGTTCATGATGGCTACTTCGA
CATGGATAAAGCATTCGATCGGCTCAAACTCAATATTTTCATCAGCTTGGCTGAACTAGAATCTGATAATATAGGTGAAC
AAGTCAAAAATGGAATCAAAGAAAAAGCGAAACAAGGTAAACTGATTACAACACATGCACCCTTTGGGTATCACTATCAT
AATGGTACATTCACGATAGACACAGTAAAAGCACCAACAGTAAAAGCTGTGTTCAATTATTACCTTCAAGGTTATGGTTA
TAAAAAAATTGCGCAATACTTAGAAGCTGATGATAAACTCATTAATCGCAAGCCCTATCAAGTGCGTAATATTATCCTTA
ACCCTAATTACTGTGGCCGTGTTATCAATCAATACGGACAATATGAAAACATGTTCCCAGCTATTGTCAGTACAACGATA
TACGAAGAAGCTCAAGTTACCCGAACTCAGAAACAAGTCAAACGTAAGCCGTCAGAAAATCAACTCAAACAAAAAATCAA
ATGTCCCTATTGTCATTCGACACTCACAAACATAACTATCCGAAAAACAGATCATTCATTGCGTTACTATGTCTGTCCGC
AAAATATGAATAATGCTCGCTTTGTTTGTGAATTTAAAGGAATCAACGCACAAGAATTAGAAACAAGTGTTTTAGCGACT
TGTCAGGACTTCTTTCAAAATCAACAGCTCTATTCAAAAATAAACCATACTATTCAACAACGACTCAAAAGACAAAGGAT
GCATGATACTAGTAACACATTAACTCAGGAAAAGCTTATTGAAAATTTAGCACAAGGAAAAATTGATGTAGAAACATTCA
GAGAACAGTCCCAATCAATTAATCTACAGAATAAACCTATACAAGCAATAAGTGATATTCGTATAAAAGCATCACTACAA
AAGGTTATACAGAAAAGTTTCACGTTAAACATGCTGAATCCCTATATTGATGAAATTCGCATTACAAAAAACAAAGCCCT
TGTTGGGATCTATTTCAAAAATGAACCATTGAACATTGTGAACCAAACCTCGCAATCATCGATTGTTTAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase (Recombinase)

(170-249)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-322)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus COL

88.196

100

0.882
  ccrA Staphylococcus aureus N315

72.383

100

0.724