Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LIT95_RS05690 Genome accession   NZ_CP085289
Coordinates   1204380..1205750 (+) Length   456 a.a.
NCBI ID   WP_126254731.1    Uniprot ID   -
Organism   Enterococcus faecalis strain EFS17     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1199380..1210750
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIT95_RS05665 (LIT95_05665) - 1199870..1200337 (-) 468 WP_033594377.1 VOC family protein -
  LIT95_RS05670 (LIT95_05670) - 1200402..1201349 (+) 948 WP_002389777.1 YafY family protein -
  LIT95_RS05675 (LIT95_05675) - 1201441..1202694 (-) 1254 WP_002385467.1 glutamate-5-semialdehyde dehydrogenase -
  LIT95_RS05680 (LIT95_05680) proB 1202681..1203496 (-) 816 WP_002389550.1 glutamate 5-kinase -
  LIT95_RS05685 (LIT95_05685) - 1203816..1204301 (+) 486 WP_002367569.1 dUTP diphosphatase -
  LIT95_RS05690 (LIT95_05690) radA 1204380..1205750 (+) 1371 WP_126254731.1 DNA repair protein RadA Machinery gene
  LIT95_RS05695 (LIT95_05695) - 1205851..1206996 (+) 1146 WP_002368622.1 PIN/TRAM domain-containing protein -
  LIT95_RS05700 (LIT95_05700) ispF 1207015..1207488 (+) 474 WP_002367570.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  LIT95_RS05705 (LIT95_05705) gltX 1207541..1208998 (+) 1458 WP_002389540.1 glutamate--tRNA ligase -
  LIT95_RS05710 (LIT95_05710) epsC 1209268..1209807 (+) 540 WP_002356054.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49743.25 Da        Isoelectric Point: 7.3489

>NTDB_id=617027 LIT95_RS05690 WP_126254731.1 1204380..1205750(+) (radA) [Enterococcus faecalis strain EFS17]
MAKKAKVQFECQSCGYVSPKYLGRCPNCGQWNSMVEEVIQDTSDRRARVSLTGKKTQPQRLSEVVPKKEPRVKTELVELN
RVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSQQLAATGGTVLYVSGEESAEQIKLRAERLGTVNETFYLYAETDMHEISR
AIEKLEPDYVIIDSIQTMTQPDVTSVAGSVSQVRETTAELLKLAKTNGVAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GDKHHTFRILRAVKNRFGSTNEIGIFEMQTHGLVEVMNPSQVFLEERLEGATGSSIVVAMEGSRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKINEPAIDLALAISIASSYKEKGTSSSECFIGEIGLTG
EIRRVNSIEQRVREAQKLGFTKVYVPKNNLGGWEAPEGIEIIGVSTIGETLRKVFK

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=617027 LIT95_RS05690 WP_126254731.1 1204380..1205750(+) (radA) [Enterococcus faecalis strain EFS17]
ATGGCAAAAAAAGCAAAAGTTCAATTTGAATGTCAAAGCTGTGGGTATGTGTCTCCAAAATATCTCGGCCGCTGTCCTAA
TTGCGGACAATGGAATTCGATGGTGGAAGAGGTTATTCAAGATACTTCTGATCGTCGTGCTAGAGTAAGTTTGACTGGTA
AAAAGACACAACCACAACGGCTATCTGAAGTCGTACCTAAAAAAGAGCCTCGTGTAAAAACTGAATTAGTGGAGCTGAAT
CGGGTTTTAGGTGGTGGCGTTGTTCCAGGTTCGTTAGTTTTAATCGGTGGCGATCCCGGAATAGGTAAGTCAACATTGCT
CTTACAAGTATCGCAACAATTAGCAGCCACAGGTGGCACGGTTTTATATGTTTCTGGTGAAGAAAGTGCTGAGCAAATTA
AATTGCGAGCGGAGCGTTTAGGCACGGTCAACGAGACATTTTACTTATACGCAGAAACAGATATGCACGAAATTTCACGA
GCCATTGAAAAATTAGAACCAGACTATGTCATTATTGACTCGATTCAAACAATGACGCAGCCAGATGTTACCAGCGTTGC
TGGTAGTGTCAGTCAAGTCCGAGAAACAACTGCCGAATTATTGAAACTGGCAAAAACGAATGGGGTTGCCATTTTTATTG
TCGGACACGTAACGAAGGAAGGGTCTATCGCAGGGCCACGGATGTTGGAACATATGGTAGACACGGTTCTTTATTTTGAA
GGAGATAAGCATCACACCTTTAGAATCTTACGGGCTGTCAAAAATCGGTTTGGCTCAACGAATGAGATTGGTATTTTTGA
AATGCAGACACATGGATTGGTTGAAGTTATGAATCCTTCTCAGGTCTTTTTAGAAGAACGTTTAGAAGGAGCCACCGGAT
CTTCAATTGTGGTAGCAATGGAAGGTTCACGCCCCATTTTGGTGGAAATCCAAGCGTTAGTTACCCCAACGATGTTTGGT
AATGCCAAGCGAACGACCACCGGGTTGGACTTTAATCGCGTTTCTTTAATCATGGCGGTTTTAGAAAAACGGGCAGGCCT
TTTATTACAAAACCAGGATGCGTATCTAAAAGCGGCTGGTGGAGTCAAAATTAATGAACCAGCTATTGATTTAGCGTTAG
CAATCAGTATTGCTTCTAGCTATAAAGAAAAAGGAACCTCATCTTCGGAATGTTTCATTGGTGAGATTGGTTTAACTGGT
GAAATTCGCCGCGTGAATAGTATTGAACAACGAGTGCGTGAAGCGCAAAAACTAGGCTTTACAAAAGTCTATGTCCCTAA
AAATAACCTTGGTGGCTGGGAAGCGCCTGAAGGTATTGAAATTATTGGCGTTTCAACAATTGGTGAAACGTTAAGAAAAG
TTTTTAAATGA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

AAA domain (AAA_25)

(65-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

75.165

99.781

0.75
  radA Streptococcus pneumoniae Rx1

75.165

99.781

0.75
  radA Streptococcus pneumoniae D39

75.165

99.781

0.75
  radA Streptococcus pneumoniae R6

75.165

99.781

0.75
  radA Streptococcus pneumoniae TIGR4

75.165

99.781

0.75
  radA Streptococcus mitis SK321

75.165

99.781

0.75
  radA Bacillus subtilis subsp. subtilis str. 168

66.075

98.904

0.654