Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LG333_RS14890 Genome accession   NZ_CP085270
Coordinates   2858629..2859699 (-) Length   356 a.a.
NCBI ID   WP_223490131.1    Uniprot ID   -
Organism   Sutcliffiella horikoshii strain ABH-536     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2853629..2864699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG333_RS14840 (LG333_14765) - 2853878..2854144 (-) 267 WP_010194663.1 phosphocarrier protein HPr -
  LG333_RS14845 (LG333_14770) - 2854555..2854743 (-) 189 WP_223490115.1 YqzE family protein -
  LG333_RS14850 (LG333_14775) - 2854775..2855290 (-) 516 WP_404468459.1 shikimate kinase -
  LG333_RS14855 (LG333_14780) - 2855403..2855633 (-) 231 WP_223490119.1 YuzF family protein -
  LG333_RS14860 (LG333_14785) comGG 2855696..2856124 (-) 429 WP_404468460.1 competence type IV pilus minor pilin ComGG -
  LG333_RS14865 (LG333_14790) comGF 2856078..2856524 (-) 447 WP_404468461.1 competence type IV pilus minor pilin ComGF -
  LG333_RS14870 (LG333_14795) - 2856508..2856843 (-) 336 WP_404468462.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LG333_RS14875 (LG333_14800) comGD 2856827..2857285 (-) 459 WP_404468463.1 competence type IV pilus minor pilin ComGD -
  LG333_RS14880 (LG333_14805) comGC 2857272..2857607 (-) 336 WP_404468465.1 competence type IV pilus major pilin ComGC -
  LG333_RS14885 (LG333_14810) comGB 2857608..2858645 (-) 1038 WP_404468466.1 competence type IV pilus assembly protein ComGB -
  LG333_RS14890 (LG333_14815) comGA 2858629..2859699 (-) 1071 WP_223490131.1 competence type IV pilus ATPase ComGA Machinery gene
  LG333_RS14895 (LG333_14820) - 2859927..2860754 (-) 828 WP_404468467.1 serine hydrolase -
  LG333_RS14900 (LG333_14825) - 2860741..2861760 (-) 1020 WP_223490135.1 ABC transporter ATP-binding protein -
  LG333_RS14905 (LG333_14830) - 2861763..2862686 (-) 924 WP_404468469.1 NlpC/P60 family protein -
  LG333_RS14910 (LG333_14835) - 2862679..2863779 (-) 1101 WP_404468471.1 mandelate racemase/muconate lactonizing enzyme family protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40813.30 Da        Isoelectric Point: 9.0484

>NTDB_id=616767 LG333_RS14890 WP_223490131.1 2858629..2859699(-) (comGA) [Sutcliffiella horikoshii strain ABH-536]
MNIEKKCEEIIRQAIRLRVSDIHIKPHETSAKVLFRLDHYLYDQEDLPLEIYERILSHLKFQAEMDIGETRKPQNGALNL
FIDSKHINLRLSTLPTVNQESLVIRILPHDDNQFPLKRLSLFPNSTRKLFSLMKHSHGLVLFTGPTGSGKTTTLYSILEE
SKGMLQRNIITLEDPVERRSKNVLQVQVNEKAGITYATGLKAILRHDPDIIMVGEIRDEETAKIAIRASLTGHLVLSTLH
TRDAKGAVHRLLEFGVTQQELEQTLIAISAQRLVELKCPYCHGDCTSFCRKYRQHRLASVYELLYGRELSKVMEECKGAK
VELRYPTLKEVIKKGIALGFIHQKEYEKWVNDGKGQ

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=616767 LG333_RS14890 WP_223490131.1 2858629..2859699(-) (comGA) [Sutcliffiella horikoshii strain ABH-536]
ATGAATATTGAAAAAAAGTGTGAAGAGATCATTCGCCAAGCTATCCGTTTGCGTGTATCAGATATTCACATCAAACCACA
TGAAACGTCCGCCAAAGTACTTTTCCGTTTGGACCACTACCTCTATGATCAAGAAGATCTCCCACTAGAAATCTATGAAC
GGATTTTATCTCACCTTAAATTCCAAGCCGAAATGGACATAGGTGAAACAAGAAAGCCCCAGAATGGCGCATTAAACCTT
TTTATCGACTCCAAACATATCAATCTGCGACTCTCGACCTTGCCCACTGTTAATCAAGAAAGTCTAGTCATCAGAATACT
GCCGCATGATGACAACCAATTCCCTTTAAAACGTTTATCCCTGTTTCCGAACTCCACAAGAAAACTATTTTCATTAATGA
AGCACTCCCACGGGCTTGTTCTGTTCACTGGTCCGACCGGCTCTGGCAAAACCACCACTCTGTATTCGATTTTGGAAGAG
TCTAAGGGGATGCTGCAACGAAATATTATTACACTTGAGGACCCGGTGGAGCGGAGAAGCAAAAATGTGCTTCAGGTGCA
GGTGAATGAAAAGGCGGGGATCACCTATGCGACAGGTTTGAAGGCTATCCTCCGACATGATCCAGACATAATTATGGTCG
GGGAAATCAGGGATGAAGAAACGGCGAAGATCGCTATAAGGGCATCGTTAACGGGTCATTTAGTATTAAGCACGTTGCAT
ACGCGCGATGCTAAAGGTGCTGTGCATCGGCTGTTGGAGTTTGGGGTCACGCAACAGGAATTGGAACAAACATTAATCGC
TATTTCGGCACAAAGGCTTGTGGAGTTGAAATGTCCATATTGCCATGGGGATTGCACATCTTTTTGCAGAAAGTACAGGC
AACATCGATTGGCCAGTGTATATGAACTTTTATATGGGCGGGAACTGTCCAAGGTGATGGAAGAGTGTAAAGGGGCAAAG
GTGGAATTACGCTATCCCACACTAAAGGAAGTGATTAAAAAGGGGATAGCACTGGGCTTTATTCATCAAAAGGAATATGA
AAAGTGGGTGAACGATGGCAAGGGGCAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.857

98.315

0.559