Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NUI00_RS19950 Genome accession   NZ_CP102488
Coordinates   4118319..4119815 (+) Length   498 a.a.
NCBI ID   WP_001040619.1    Uniprot ID   -
Organism   Escherichia coli O15:H18 strain ST69     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4113319..4124815
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUI00_RS19920 (NUI00_19925) - 4113572..4113880 (+) 309 WP_000385056.1 hypothetical protein -
  NUI00_RS19925 (NUI00_19930) zntA 4113913..4116111 (-) 2199 WP_000106497.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  NUI00_RS19930 (NUI00_19935) yhhN 4116185..4116811 (-) 627 WP_000964718.1 lysoplasmalogenase -
  NUI00_RS19935 (NUI00_19940) yhhM 4116952..4117311 (+) 360 WP_000042883.1 DUF2500 domain-containing protein -
  NUI00_RS19940 (NUI00_19945) yhhL 4117314..4117583 (-) 270 WP_001295207.1 DUF1145 family protein -
  NUI00_RS19945 (NUI00_19950) rsmD 4117573..4118169 (-) 597 WP_000743195.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  NUI00_RS19950 (NUI00_19955) pilA 4118319..4119815 (+) 1497 WP_001040619.1 signal recognition particle-docking protein FtsY Machinery gene
  NUI00_RS19955 (NUI00_19960) ftsE 4119818..4120486 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  NUI00_RS19960 (NUI00_19965) ftsX 4120479..4121537 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  NUI00_RS19965 (NUI00_19970) rpoH 4121782..4122636 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  NUI00_RS19970 (NUI00_19975) livJ 4122908..4124011 (+) 1104 WP_001021986.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  NUI00_RS26800 - 4124071..4124170 (+) 100 Protein_3927 hypothetical protein -
  NUI00_RS19975 (NUI00_19980) panM 4124211..4124594 (-) 384 WP_000778800.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54668.50 Da        Isoelectric Point: 4.1468

>NTDB_id=616675 NUI00_RS19950 WP_001040619.1 4118319..4119815(+) (pilA) [Escherichia coli O15:H18 strain ST69]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVPAQEPAKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVVIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=616675 NUI00_RS19950 WP_001040619.1 4118319..4119815(+) (pilA) [Escherichia coli O15:H18 strain ST69]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCCGGCGCAAGAGCCTGCGAAGGCTTCTGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGTTAT
CGAGCGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAACCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAGCAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTAGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAGGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTTCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378