Detailed information    

insolico Bioinformatically predicted

Overview

Name   recP/tkt   Type   Machinery gene
Locus tag   NR349_RS14355 Genome accession   NZ_CP102380
Coordinates   2950748..2952739 (-) Length   663 a.a.
NCBI ID   WP_000098614.1    Uniprot ID   B7UHY1
Organism   Escherichia coli strain BM28     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2945748..2957739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NR349_RS14330 (NR349_14330) fumE 2945790..2946299 (-) 510 WP_000224147.1 fumarase E -
  NR349_RS14335 (NR349_14335) glpX 2946321..2947286 (-) 966 WP_000987283.1 class II fructose-bisphosphatase -
  NR349_RS14340 (NR349_14340) yggP 2947283..2948560 (-) 1278 WP_000853247.1 zinc-binding dehydrogenase -
  NR349_RS14345 (NR349_14345) cmtA 2948575..2949963 (-) 1389 WP_000428805.1 PTS mannitol transporter subunit IICB -
  NR349_RS14350 (NR349_14350) cmtB 2949991..2950434 (-) 444 WP_001239650.1 PTS mannitol transporter subunit IIA -
  NR349_RS14355 (NR349_14355) recP/tkt 2950748..2952739 (-) 1992 WP_000098614.1 transketolase Machinery gene
  NR349_RS14360 (NR349_14360) loiP 2953017..2953775 (+) 759 WP_001326497.1 metalloprotease LoiP -
  NR349_RS14365 (NR349_14365) speB 2953981..2954901 (-) 921 WP_000105566.1 agmatinase -
  NR349_RS14370 (NR349_14370) speA 2955039..2957015 (-) 1977 WP_001300904.1 biosynthetic arginine decarboxylase -
  NR349_RS14375 (NR349_14375) yqgB 2957024..2957155 (-) 132 WP_001297406.1 acid stress response protein YqgB -
  NR349_RS22000 yqgG 2957249..2957392 (-) 144 WP_001297404.1 protein YqgG -

Sequence

Protein


Download         Length: 663 a.a.        Molecular weight: 72211.74 Da        Isoelectric Point: 5.4041

>NTDB_id=616385 NR349_RS14355 WP_000098614.1 2950748..2952739(-) (recP/tkt) [Escherichia coli strain BM28]
MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDFLKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGY
DLPMEELKNFRQLHSKTPGHPEVGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDGHDAASIKRAVEEARAVTDKPSL
LMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQLGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQ
EAAEFTRRMKGEMPSDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSGSKAINEDA
AGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAALMKQRQVMVYTHDSIGLGEDGPTHQPVEQVA
SLRVTPNMSTWRPCDQVESAVAWKYGVERQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSE
VELAVAAYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVGLNGAIVGMTTFGESAP
AELLFEEFGFTVDNVVAKAKELL

Nucleotide


Download         Length: 1992 bp        

>NTDB_id=616385 NR349_RS14355 WP_000098614.1 2950748..2952739(-) (recP/tkt) [Escherichia coli strain BM28]
ATGTCCTCACGTAAAGAGCTTGCCAATGCTATTCGTGCGCTGAGCATGGACGCAGTACAGAAAGCCAAATCCGGTCACCC
GGGTGCCCCTATGGGTATGGCTGACATTGCCGAAGTCCTGTGGCGTGATTTCCTGAAACACAACCCGCAGAATCCGTCCT
GGGCTGACCGTGACCGCTTCGTGCTGTCCAACGGCCACGGCTCCATGCTGATCTACAGCCTGCTGCACCTCACCGGTTAC
GATCTGCCGATGGAAGAACTGAAAAACTTCCGTCAGCTGCACTCTAAAACTCCGGGTCACCCGGAAGTGGGTTACACCGC
TGGTGTGGAAACCACCACCGGTCCGCTGGGTCAGGGTATTGCCAACGCAGTCGGTATGGCGATTGCAGAAAAAACGCTGG
CGGCGCAGTTTAACCGTCCGGGCCACGACATTGTCGACCACTACACCTACGCCTTCATGGGCGACGGCTGCATGATGGAA
GGCATCTCCCACGAAGTTTGCTCTCTGGCGGGTACGCTGAAGCTGGGTAAACTGATTGCATTCTACGATGACAACGGTAT
TTCTATCGATGGTCACGTTGAAGGCTGGTTCACCGACGACACCGCAATGCGTTTCGAAGCTTACGGCTGGCACGTTATTC
GCGACATCGACGGTCATGACGCGGCATCTATCAAACGCGCAGTAGAAGAAGCGCGCGCAGTGACTGACAAACCTTCCCTG
CTGATGTGCAAAACCATCATCGGTTTCGGTTCCCCGAACAAAGCCGGTACCCACGACTCCCACGGTGCGCCGCTGGGCGA
CGCTGAAATTGCCCTGACCCGCGAACAACTGGGCTGGAAATATGCGCCGTTCGAAATCCCGTCTGAAATCTATGCTCAGT
GGGATGCGAAAGAAGCAGGCCAGGCGAAAGAATCCGCATGGAACGAGAAATTCGCTGCTTACGCGAAAGCTTATCCGCAG
GAAGCCGCTGAATTTACCCGCCGTATGAAAGGCGAAATGCCGTCTGACTTCGACGCTAAAGCGAAAGAGTTCATCGCTAA
ACTGCAGGCTAATCCGGCGAAAATCGCCAGCCGTAAAGCGTCTCAGAATGCTATCGAAGCGTTCGGTCCGCTGTTGCCGG
AATTCCTCGGCGGTTCTGCTGACCTGGCGCCGTCTAACCTGACCCTGTGGTCTGGTTCTAAAGCAATCAACGAAGATGCT
GCGGGTAACTACATCCACTACGGTGTTCGCGAGTTCGGTATGACCGCGATTGCTAACGGTATCTCCCTGCACGGTGGCTT
CCTGCCGTACACCTCCACCTTCCTGATGTTCGTGGAATACGCACGTAACGCCGTACGTATGGCTGCGCTGATGAAACAGC
GTCAGGTGATGGTTTACACCCACGACTCCATCGGTCTGGGCGAAGACGGCCCGACTCACCAGCCGGTTGAGCAGGTCGCT
TCTCTGCGCGTAACCCCGAACATGTCTACATGGCGTCCGTGTGACCAGGTTGAATCCGCGGTCGCGTGGAAATACGGTGT
TGAGCGTCAGGACGGCCCGACCGCACTGATCCTCTCCCGTCAGAACCTGGCGCAGCAGGAACGAACTGAAGAGCAACTGG
CAAACATCGCGCGCGGTGGTTATGTGCTGAAAGACTGCGCCGGTCAGCCGGAACTGATTTTCATCGCTACCGGTTCAGAA
GTTGAACTGGCTGTTGCTGCCTACGAAAAACTGACTGCCGAAGGCGTGAAAGCGCGCGTGGTGTCCATGCCGTCTACCGA
CGCATTTGACAAGCAGGATGCTGCTTACCGTGAATCCGTACTGCCGAAAGCGGTTACTGCACGCGTTGCTGTAGAAGCGG
GTATTGCTGACTACTGGTACAAGTATGTTGGCCTGAACGGTGCTATCGTCGGTATGACCACCTTCGGTGAATCTGCTCCG
GCAGAGCTGCTGTTTGAAGAGTTCGGCTTCACTGTTGATAACGTTGTTGCGAAAGCAAAAGAACTGCTGTAA

Domains

Predicted by InterProScan.

Transketolase, C-terminal domain (Transketolase_C)

(540-655)

Transketolase, pyrimidine binding domain (Transket_pyr)

(354-524)

Transketolase, thiamine diphosphate binding domain (Transketolase_N)

(4-334)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7UHY1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recP/tkt Streptococcus pneumoniae TIGR4

48.024

99.246

0.477