Detailed information NTG0616319

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: NR350_RS16975
• Genome accession: NZ_CP102379
• Coordinates: 3473857..3475350 (-)
• Length: 497 a.a.
• NCBI ID: WP_001040654.1
• Uniprot ID: P10121
• Organism: Escherichia coli strain BM28 lysU
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3468857..3480350

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NR350_RS16950 (NR350_16950)	panM	3469091..3469474 (+)	384	WP_000778768.1	aspartate 1-decarboxylase autocleavage activator PanM	-
NR350_RS16955 (NR350_16955)	livJ	3469662..3470765 (-)	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
NR350_RS16960 (NR350_16960)	rpoH	3471036..3471890 (-)	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
NR350_RS16965 (NR350_16965)	ftsX	3472135..3473193 (-)	1059	WP_001042003.1	permease-like cell division protein FtsX	-
NR350_RS16970 (NR350_16970)	ftsE	3473186..3473854 (-)	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
NR350_RS16975 (NR350_16975)	pilA	3473857..3475350 (-)	1494	WP_001040654.1	signal recognition particle-docking protein FtsY	Machinery gene
NR350_RS16980 (NR350_16980)	rsmD	3475500..3476096 (+)	597	WP_000743193.1	16S rRNA (guanine(966)-N(2))-methyltransferase	-
NR350_RS16985 (NR350_16985)	yhhL	3476086..3476355 (+)	270	WP_001311191.1	DUF1145 family protein	-
NR350_RS16990 (NR350_16990)	yhhM	3476358..3476717 (-)	360	WP_000042895.1	DUF2500 domain-containing protein	-
NR350_RS16995 (NR350_16995)	yhhN	3476858..3477484 (+)	627	WP_000964718.1	lysoplasmalogenase	-
NR350_RS17000 (NR350_17000)	zntA	3477558..3479756 (+)	2199	WP_000106551.1	Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA	-
NR350_RS17005 (NR350_17005)	tusA	3479858..3480103 (-)	246	WP_000130621.1	sulfurtransferase TusA	-

Sequence

Protein

• Length: 497 a.a.
• Molecular weight: 54513.35 Da
• Isoelectric Point: 4.1560

>NTDB_id=616319 NR350_RS16975 WP_001040654.1 3473857..3475350(-) (pilA) [Escherichia coli strain BM28 lysU]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELETALA
AEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIADV
GVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKAPFVILMVGVNGVGKTTTIGKLARQFEQQ
GKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKKI
VRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDL
RPFKADDFIEALFARED

Nucleotide

• Length: 1494 bp

>NTDB_id=616319 NR350_RS16975 WP_001040654.1 3473857..3475350(-) (pilA) [Escherichia coli strain BM28 lysU]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCCTCTGAACAAGCCGTTGAAG
AGCAGCCGCAAGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATTACCGACGAAGAGCTGGAAACGGCGCTGGCT
GCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCA
GGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCGGAT
TTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTG
GGTGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGACGCCGAGGCGCT
CTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGT
TTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAGCAG
GGTAAATCGGTGATGCTGGCGGCGGGTGATACTTTCCGTGCAGCTGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCGCAA
CAATATTCCGGTGATTGCCCAGCATACCGGGGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAGCGC
GTAATATCGACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATC
GTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAACGC
GGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGAAAG
GCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGATTTG
CGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

• SRP54-type protein, helical bundle domain (SRP54_N) (210-275)

• SRP54-type protein, GTPase domain (SRP54) (293-493)

3D structure

• PDB: 1FTS
• PDB: 2QY9
• PDB: 2XXA
• PDB: 2YHS
• PDB: 3ZN8
• PDB: 4C7O
• PDB: 5GAD
• PDB: 5NCO
• PDB: 5NIY
• PDB: 6CQP
• PDB: 6CS8
• PDB: 6CVD
• PDB: 6DLX
• PDB: 6FPK
• PDB: 6FPR
• PDB: 6FQD
• PDB: 6N5I
• PDB: 6N5J
• PDB: 6N6N
• PDB: 6N9B
• PDB: 6NC1
• PDB: 6NC4
• PDB: 7O9H

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	48.698	77.264	0.376