Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LG307_RS15010 Genome accession   NZ_CP085237
Coordinates   2857883..2858953 (-) Length   356 a.a.
NCBI ID   WP_404443523.1    Uniprot ID   -
Organism   Sutcliffiella horikoshii strain ADH2-198     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2816015..2861748 2857883..2858953 within 0


Gene organization within MGE regions


Location: 2816015..2861748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG307_RS14685 (LG307_14615) - 2817252..2817518 (-) 267 WP_010194663.1 phosphocarrier protein HPr -
  LG307_RS14690 (LG307_14620) - 2817863..2818045 (-) 183 WP_404443369.1 YqzE family protein -
  LG307_RS14695 (LG307_14625) - 2818079..2818594 (-) 516 WP_404443371.1 shikimate kinase -
  LG307_RS14700 (LG307_14630) - 2818694..2818921 (-) 228 WP_404443373.1 YuzF family protein -
  LG307_RS14705 (LG307_14635) comGG 2818996..2819412 (-) 417 WP_404443376.1 competence type IV pilus minor pilin ComGG -
  LG307_RS14710 (LG307_14640) comGF 2819366..2819812 (-) 447 WP_404443378.1 competence type IV pilus minor pilin ComGF -
  LG307_RS14715 (LG307_14645) comGE 2819796..2820131 (-) 336 WP_404443381.1 competence type IV pilus minor pilin ComGE -
  LG307_RS14720 (LG307_14650) comGD 2820115..2820573 (-) 459 WP_404443383.1 competence type IV pilus minor pilin ComGD -
  LG307_RS14725 (LG307_14655) - 2820560..2820745 (-) 186 WP_404443387.1 hypothetical protein -
  LG307_RS14730 (LG307_14660) - 2820788..2822452 (-) 1665 WP_404443389.1 recombinase family protein -
  LG307_RS14735 (LG307_14665) - 2822526..2822786 (-) 261 WP_404443392.1 hypothetical protein -
  LG307_RS14740 (LG307_14670) - 2822910..2823245 (-) 336 WP_404443395.1 hypothetical protein -
  LG307_RS14745 (LG307_14675) - 2823257..2823478 (-) 222 WP_404443397.1 hypothetical protein -
  LG307_RS14750 (LG307_14680) - 2823607..2823846 (+) 240 WP_404443400.1 helix-turn-helix domain-containing protein -
  LG307_RS14755 (LG307_14685) - 2823839..2824117 (+) 279 WP_404443402.1 hypothetical protein -
  LG307_RS14760 (LG307_14690) - 2824139..2824540 (-) 402 WP_404443404.1 hypothetical protein -
  LG307_RS14765 (LG307_14695) - 2824635..2824847 (-) 213 WP_404443406.1 helix-turn-helix domain-containing protein -
  LG307_RS14770 (LG307_14700) - 2825112..2825420 (+) 309 WP_404443408.1 hypothetical protein -
  LG307_RS14775 (LG307_14705) - 2825427..2826596 (+) 1170 WP_404443410.1 FtsK/SpoIIIE domain-containing protein -
  LG307_RS14780 (LG307_14710) - 2826580..2827254 (+) 675 WP_404443412.1 hypothetical protein -
  LG307_RS14785 (LG307_14715) - 2827271..2827885 (-) 615 WP_404443414.1 restriction endonuclease -
  LG307_RS14790 (LG307_14720) - 2828004..2828156 (+) 153 WP_404443416.1 hypothetical protein -
  LG307_RS14795 (LG307_14725) - 2828229..2828561 (+) 333 WP_404443418.1 hypothetical protein -
  LG307_RS14800 (LG307_14730) - 2828558..2829430 (+) 873 WP_404443421.1 hypothetical protein -
  LG307_RS14805 (LG307_14735) - 2829753..2832038 (-) 2286 WP_404443424.1 phage tail tape measure protein -
  LG307_RS14810 (LG307_14740) - 2832042..2832287 (-) 246 WP_404443427.1 hypothetical protein -
  LG307_RS14815 (LG307_14745) - 2832353..2832682 (-) 330 WP_404443429.1 hypothetical protein -
  LG307_RS14820 (LG307_14750) - 2832739..2833284 (-) 546 WP_404443431.1 hypothetical protein -
  LG307_RS14825 (LG307_14755) - 2833292..2833738 (-) 447 WP_404443433.1 hypothetical protein -
  LG307_RS14830 (LG307_14760) - 2833735..2834067 (-) 333 WP_404443436.1 hypothetical protein -
  LG307_RS14835 (LG307_14765) - 2834070..2834390 (-) 321 WP_404443438.1 hypothetical protein -
  LG307_RS14840 (LG307_14770) - 2834387..2834701 (-) 315 WP_404443441.1 phage head-tail connector protein -
  LG307_RS14845 (LG307_14775) - 2834701..2834898 (-) 198 WP_404443443.1 hypothetical protein -
  LG307_RS14850 (LG307_14780) - 2834963..2835781 (-) 819 WP_404443445.1 hypothetical protein -
  LG307_RS14855 (LG307_14785) - 2835864..2836514 (-) 651 WP_404443448.1 DUF4355 domain-containing protein -
  LG307_RS14860 (LG307_14790) - 2836632..2837498 (-) 867 WP_404443451.1 minor capsid protein -
  LG307_RS14865 (LG307_14795) - 2837482..2838804 (-) 1323 WP_404443454.1 phage portal protein -
  LG307_RS14870 (LG307_14800) - 2838820..2839470 (-) 651 WP_404443457.1 hypothetical protein -
  LG307_RS14875 (LG307_14805) - 2839463..2840512 (-) 1050 WP_404443460.1 hypothetical protein -
  LG307_RS14880 (LG307_14810) - 2840896..2841297 (-) 402 WP_404443462.1 helix-turn-helix domain-containing protein -
  LG307_RS14885 (LG307_14815) - 2841337..2841570 (-) 234 WP_404443464.1 hypothetical protein -
  LG307_RS14890 (LG307_14820) - 2841575..2842405 (-) 831 WP_404443467.1 phage tail domain-containing protein -
  LG307_RS14895 (LG307_14825) - 2842374..2842637 (-) 264 WP_404443470.1 hypothetical protein -
  LG307_RS14900 (LG307_14830) - 2842710..2843084 (-) 375 WP_404443472.1 hypothetical protein -
  LG307_RS14905 (LG307_14835) - 2843376..2843798 (+) 423 WP_404443475.1 hypothetical protein -
  LG307_RS14910 (LG307_14840) - 2844011..2844793 (-) 783 WP_404443477.1 hypothetical protein -
  LG307_RS14915 (LG307_14845) - 2844863..2845219 (-) 357 WP_404443478.1 DUF2513 domain-containing protein -
  LG307_RS14920 (LG307_14850) - 2845317..2845805 (-) 489 WP_404443481.1 hypothetical protein -
  LG307_RS14925 (LG307_14855) - 2845819..2846256 (-) 438 WP_404443483.1 hypothetical protein -
  LG307_RS14930 (LG307_14860) - 2846459..2846659 (-) 201 WP_404443486.1 hypothetical protein -
  LG307_RS14935 (LG307_14865) - 2846656..2846805 (-) 150 WP_404443489.1 hypothetical protein -
  LG307_RS14940 (LG307_14870) - 2846802..2847575 (-) 774 WP_404443491.1 hypothetical protein -
  LG307_RS14945 (LG307_14875) - 2847588..2847977 (-) 390 WP_404443494.1 hypothetical protein -
  LG307_RS14950 (LG307_14880) - 2848308..2848520 (-) 213 WP_404443496.1 helix-turn-helix domain-containing protein -
  LG307_RS14955 (LG307_14885) - 2848818..2851490 (-) 2673 WP_404443499.1 hypothetical protein -
  LG307_RS14960 (LG307_14890) - 2851725..2853221 (-) 1497 WP_404443500.1 hypothetical protein -
  LG307_RS14965 (LG307_14895) - 2853264..2853425 (-) 162 WP_404443502.1 hypothetical protein -
  LG307_RS14970 (LG307_14900) - 2853492..2853680 (-) 189 WP_404443505.1 helix-turn-helix domain-containing protein -
  LG307_RS14975 (LG307_14905) - 2853783..2854562 (-) 780 WP_404443507.1 hypothetical protein -
  LG307_RS14980 (LG307_14910) - 2854668..2854982 (-) 315 WP_404443510.1 hypothetical protein -
  LG307_RS14985 (LG307_14915) - 2855131..2855424 (-) 294 WP_404443512.1 hypothetical protein -
  LG307_RS14990 (LG307_14920) - 2855439..2855678 (-) 240 WP_404443515.1 hypothetical protein -
  LG307_RS14995 (LG307_14925) - 2855947..2856567 (-) 621 WP_404443518.1 helix-turn-helix domain-containing protein -
  LG307_RS15000 - 2856702..2856860 (-) 159 Protein_2917 competence type IV pilus major pilin ComGC -
  LG307_RS15005 (LG307_14935) comGB 2856862..2857899 (-) 1038 WP_404443520.1 competence type IV pilus assembly protein ComGB -
  LG307_RS15010 (LG307_14940) comGA 2857883..2858953 (-) 1071 WP_404443523.1 competence type IV pilus ATPase ComGA Machinery gene
  LG307_RS15015 (LG307_14945) - 2859200..2860018 (-) 819 WP_404443526.1 serine hydrolase -
  LG307_RS15020 (LG307_14950) - 2860005..2861024 (-) 1020 WP_404443529.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40734.30 Da        Isoelectric Point: 8.7317

>NTDB_id=616228 LG307_RS15010 WP_404443523.1 2857883..2858953(-) (comGA) [Sutcliffiella horikoshii strain ADH2-198]
MNIEKKCEDIVRQAVRLQVSDIHIKPLEASAKVLFRLDHYLYDQEELPLEIYERMLSHLKFQAEMDIGEIRKPQNGALKL
YVDSKQINLRLSTLPTVNQESLVIRILPHDENQFPLKHLSLFPNSTRKLFSLMKHSHGLVLFTGPTGSGKTTTLYSILEE
SKGLLQRNIITLEDPVERKSRHVLQVQVNEKAGITYASGLKAILRHDPDIIMVGEIRDEETAKIAIRASLTGHLVLSTLH
TRDAKGAVHRLLEFGVTQQELEQTLIAISAQRLVELKCPYCQGECTTFCRKYRNHRLASVYELLYGRELTKVMEECKGAQ
VQLCYPTLKEVIKKGIALGFIHQREYERWVTDGKGK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=616228 LG307_RS15010 WP_404443523.1 2857883..2858953(-) (comGA) [Sutcliffiella horikoshii strain ADH2-198]
ATGAATATTGAAAAAAAATGCGAAGACATCGTTCGCCAAGCAGTCCGTTTACAAGTTTCGGATATTCACATCAAACCCCT
TGAAGCGTCCGCAAAAGTACTTTTTCGATTGGACCACTACCTCTATGACCAAGAAGAGCTTCCACTTGAAATCTATGAAA
GAATGCTGTCCCACTTAAAGTTTCAGGCCGAAATGGACATTGGAGAAATAAGAAAACCCCAAAATGGCGCCTTGAAACTA
TATGTTGACTCCAAACAAATCAACCTTCGACTCTCAACTTTGCCGACCGTTAACCAAGAAAGTTTGGTTATCCGTATCCT
GCCGCACGATGAAAACCAATTTCCTTTGAAACATCTCTCTCTTTTTCCTAATTCAACAAGAAAACTATTTTCCCTTATGA
AGCATTCCCACGGGCTTGTTTTGTTTACCGGGCCTACTGGATCTGGGAAAACCACCACATTGTATTCCATTCTCGAAGAA
TCCAAGGGATTGCTGCAACGGAATATCATCACATTAGAAGATCCTGTTGAGCGAAAAAGCAGGCATGTTCTGCAAGTTCA
GGTAAATGAAAAAGCGGGTATCACCTATGCTTCTGGGTTAAAGGCAATACTCAGGCATGACCCAGATATCATTATGGTTG
GAGAGATAAGGGATGAGGAAACAGCTAAGATTGCGATAAGGGCGTCGTTGACAGGACACTTAGTTTTAAGCACCTTGCAT
ACGAGAGATGCAAAAGGAGCGGTGCATCGCCTGTTAGAGTTTGGAGTCACTCAGCAGGAGTTGGAGCAAACATTAATAGC
AATCTCAGCACAAAGGTTAGTAGAGTTGAAATGTCCATATTGCCAAGGGGAGTGTACGACCTTTTGCAGAAAATATCGAA
ACCACAGGCTTGCAAGTGTATATGAGCTTCTGTACGGGCGGGAACTAACGAAGGTGATGGAGGAATGCAAGGGGGCACAA
GTGCAATTATGTTATCCGACCTTGAAGGAAGTAATTAAAAAAGGGATAGCGTTGGGCTTTATTCATCAGAGGGAATATGA
AAGATGGGTAACAGATGGAAAAGGCAAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.286

98.315

0.553