Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LG272_RS00390 Genome accession   NZ_CP085233
Coordinates   79853..81226 (+) Length   457 a.a.
NCBI ID   WP_404408885.1    Uniprot ID   -
Organism   Pseudidiomarina marina strain ADH-25     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 74853..86226
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG272_RS00360 (LG272_00360) - 75489..76352 (-) 864 WP_126759556.1 ATP-binding cassette domain-containing protein -
  LG272_RS00365 (LG272_00365) - 76542..77522 (+) 981 WP_404408882.1 KpsF/GutQ family sugar-phosphate isomerase -
  LG272_RS00370 (LG272_00370) kdsC 77528..78076 (+) 549 WP_404409965.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  LG272_RS00375 (LG272_00375) lptC 78073..78648 (+) 576 WP_126759559.1 LPS export ABC transporter periplasmic protein LptC -
  LG272_RS00380 (LG272_00380) lptA 78605..79138 (+) 534 WP_126759560.1 lipopolysaccharide transport periplasmic protein LptA -
  LG272_RS00385 (LG272_00385) lptB 79119..79844 (+) 726 WP_126759561.1 LPS export ABC transporter ATP-binding protein -
  LG272_RS00390 (LG272_00390) rpoN/rpoN1 79853..81226 (+) 1374 WP_404408885.1 RNA polymerase factor sigma-54 Machinery gene
  LG272_RS00395 (LG272_00395) hpf 81253..81540 (+) 288 WP_092856647.1 ribosome hibernation promoting factor -
  LG272_RS00400 (LG272_00400) ptsN 81544..81990 (+) 447 WP_404408887.1 PTS IIA-like nitrogen regulatory protein PtsN -
  LG272_RS00405 (LG272_00405) rapZ 82003..82860 (+) 858 WP_126759564.1 RNase adapter RapZ -
  LG272_RS00410 (LG272_00410) - 82885..83163 (+) 279 WP_126759565.1 HPr family phosphocarrier protein -
  LG272_RS00415 (LG272_00415) mgtE 83200..84561 (+) 1362 WP_404408890.1 magnesium transporter -
  LG272_RS00420 (LG272_00420) pmbA 84558..85904 (-) 1347 WP_404408892.1 metalloprotease PmbA -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 51322.03 Da        Isoelectric Point: 4.6524

>NTDB_id=616165 LG272_RS00390 WP_404408885.1 79853..81226(+) (rpoN/rpoN1) [Pseudidiomarina marina strain ADH-25]
MKQGLQFKFSQQLSMTPQLQQAIKLLQLSTLELQQEVQQALEENPLLELEQAEAEGETAITDDDDLWDSPCSAALGQSSS
SFDGEDSVFEGASGTTLQDHLIWQMQLSHFSAIDEAIALAIIDAIDDSGYLAVSLEEVVTAVNRQTDEPVELDEAAVVLK
RIQHFDPIGVGARDVGECLTLQLQQLPHDTPWRNEALATVREHLALLGSRDYRTLARRLRLNEDDLAAVIDLIQHLQPRP
GEAFNNSDDDYVIPDVLVRKKNNRWMVELNPESVPKLKVNESYMKLCQGTRQTDDMQYIRQQTQEAKWFIRSLESRNETL
LKVANAIVLRQQGFFEHGEEAMKPMVLADIASAVEMHESTISRVTTQKYMHSPRGVFELKYFFSSQLGTDTGGEASSTAI
RALLKKLVAAENPKKPLSDSKLTELLAEQGIQVARRTIAKYREALNIPPSSQRKRLI

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=616165 LG272_RS00390 WP_404408885.1 79853..81226(+) (rpoN/rpoN1) [Pseudidiomarina marina strain ADH-25]
ATGAAACAAGGCCTGCAGTTTAAATTCTCTCAGCAGCTGTCGATGACGCCGCAGCTGCAGCAGGCAATTAAACTTCTACA
ACTTTCGACGCTTGAACTACAACAAGAGGTTCAACAAGCGCTCGAAGAAAACCCGTTATTGGAATTAGAACAAGCTGAGG
CTGAAGGCGAAACGGCCATTACCGACGATGATGATCTTTGGGATAGTCCTTGTTCTGCGGCACTAGGTCAGTCCAGCTCG
AGCTTTGATGGCGAAGACTCAGTGTTTGAAGGGGCCTCAGGCACAACTTTGCAAGACCATTTGATTTGGCAAATGCAACT
CTCTCATTTTTCAGCTATCGATGAAGCAATTGCCCTCGCCATCATTGATGCCATTGACGATAGTGGTTACTTGGCGGTTT
CTCTGGAAGAAGTTGTAACCGCCGTAAATCGTCAAACCGATGAACCTGTTGAACTCGATGAAGCGGCTGTCGTATTAAAA
CGCATCCAACATTTTGACCCCATTGGGGTTGGTGCTCGTGATGTCGGCGAATGTCTCACCTTACAACTCCAACAATTGCC
CCATGATACGCCGTGGCGTAACGAAGCGTTAGCAACTGTGCGTGAGCATTTGGCTTTGCTTGGATCACGCGATTACCGAA
CTTTAGCACGTCGATTGCGTTTAAATGAAGACGACCTCGCTGCTGTGATTGACCTCATTCAACACCTTCAGCCGCGGCCT
GGCGAAGCGTTTAACAATAGCGACGATGACTACGTTATTCCCGATGTTTTGGTGCGCAAGAAAAATAATCGCTGGATGGT
TGAGCTAAATCCAGAAAGTGTGCCTAAACTTAAAGTGAACGAAAGTTACATGAAGTTATGCCAAGGAACGCGCCAAACCG
ACGACATGCAATACATACGCCAACAAACACAAGAAGCGAAATGGTTTATCCGCAGCCTTGAAAGCCGCAACGAAACGCTG
CTGAAGGTCGCGAATGCTATTGTATTGCGTCAACAAGGATTCTTTGAACACGGTGAAGAAGCAATGAAACCAATGGTGTT
AGCGGATATTGCAAGCGCTGTTGAGATGCATGAATCAACTATTTCTCGAGTAACCACGCAAAAGTATATGCATTCACCGC
GTGGTGTGTTTGAATTGAAGTACTTCTTCTCAAGTCAGCTCGGTACTGATACTGGAGGGGAAGCTTCATCAACGGCCATT
CGGGCGTTGTTGAAAAAGCTAGTTGCTGCGGAAAACCCGAAAAAGCCGCTGAGTGACAGTAAACTAACTGAACTCCTCGC
GGAACAAGGTATCCAAGTAGCTAGACGGACCATTGCGAAGTATCGAGAGGCGCTCAATATTCCGCCCTCGAGTCAACGCA
AACGTCTTATCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(93-283)

Sigma-54, DNA binding domain (Sigma54_DBD)

(297-455)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.95

100

0.497