Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NQZ80_RS01480 Genome accession   NZ_CP102244
Coordinates   327939..329435 (+) Length   498 a.a.
NCBI ID   WP_024216280.1    Uniprot ID   -
Organism   Escherichia coli O7:H15 strain ST70     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 322939..334435
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQZ80_RS01450 (NQZ80_01450) tusA 323186..323431 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  NQZ80_RS01455 (NQZ80_01455) zntA 323533..325731 (-) 2199 WP_024216281.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  NQZ80_RS01460 (NQZ80_01460) yhhN 325805..326431 (-) 627 WP_000964718.1 lysoplasmalogenase -
  NQZ80_RS01465 (NQZ80_01465) yhhM 326572..326931 (+) 360 WP_000042886.1 DUF2500 domain-containing protein -
  NQZ80_RS01470 (NQZ80_01470) yhhL 326934..327203 (-) 270 WP_001295207.1 DUF1145 family protein -
  NQZ80_RS01475 (NQZ80_01475) rsmD 327193..327789 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  NQZ80_RS01480 (NQZ80_01480) pilA 327939..329435 (+) 1497 WP_024216280.1 signal recognition particle-docking protein FtsY Machinery gene
  NQZ80_RS01485 (NQZ80_01485) ftsE 329438..330106 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  NQZ80_RS01490 (NQZ80_01490) ftsX 330099..331157 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  NQZ80_RS01495 (NQZ80_01495) rpoH 331402..332256 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  NQZ80_RS01500 (NQZ80_01500) livJ 332528..333631 (+) 1104 WP_024216279.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  NQZ80_RS01505 (NQZ80_01505) - 333906..334190 (+) 285 WP_024043354.1 DUF1778 domain-containing protein -
  NQZ80_RS01510 (NQZ80_01510) - 334197..334394 (+) 198 Protein_301 N-acetyltransferase -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54637.45 Da        Isoelectric Point: 4.1830

>NTDB_id=615929 NQZ80_RS01480 WP_024216280.1 327939..329435(+) (pilA) [Escherichia coli O7:H15 strain ST70]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEHAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPENVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
AAEVAEEAVMVVSPAEEDQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=615929 NQZ80_RS01480 WP_024216280.1 327939..329435(+) (pilA) [Escherichia coli O7:H15 strain ST70]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCAGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCTGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTTACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTTGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGCCGGAAAACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCCGGAGGCACAGGCGCTG
GCTGCCGAAGTGGCAGAAGAGGCGGTAATGGTGGTTTCTCCGGCAGAAGAAGATCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACTCGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCACGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCAGCGGTTGAGCAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCACAGCATACCGGGGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

49.33

74.9

0.369