Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LIN99_RS00510 Genome accession   NZ_CP085093
Coordinates   99281..99733 (+) Length   150 a.a.
NCBI ID   WP_005586498.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans strain 23S     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 94281..104733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIN99_RS00495 pnp 95238..97367 (-) 2130 WP_249850157.1 polyribonucleotide nucleotidyltransferase -
  LIN99_RS00500 nanQ 97661..98131 (+) 471 WP_005547536.1 N-acetylneuraminate anomerase -
  LIN99_RS00505 ampD 98605..99159 (-) 555 WP_005567973.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LIN99_RS00510 pilA 99281..99733 (+) 453 WP_005586498.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LIN99_RS00515 pilB 99760..101169 (+) 1410 WP_005567970.1 GspE/PulE family protein Machinery gene
  LIN99_RS00520 pilC 101162..102385 (+) 1224 WP_005567968.1 type II secretion system F family protein Machinery gene
  LIN99_RS00525 - 102382..103071 (+) 690 WP_005547547.1 A24 family peptidase -
  LIN99_RS00530 coaE 103121..103744 (+) 624 WP_005547548.1 dephospho-CoA kinase -
  LIN99_RS00535 yacG 103734..103946 (+) 213 WP_005567966.1 DNA gyrase inhibitor YacG -
  LIN99_RS00540 - 103946..104218 (+) 273 WP_005547552.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15547.96 Da        Isoelectric Point: 9.1384

>NTDB_id=615443 LIN99_RS00510 WP_005586498.1 99281..99733(+) (pilA) [Aggregatibacter actinomycetemcomitans strain 23S]
MKIQSAFISLKFVKKGFTLIELMIVIAIVAILATVAVPSYQNYTKKAAVSELLQASAPLRSEVELCIYNTGKAENCSGGQ
NGIQADVADASKQKYVKSTAVKGGVITVTGKGSLDKISYTLTASGTAASGVSWNAACGNNADIFPAGFCS

Nucleotide


Download         Length: 453 bp        

>NTDB_id=615443 LIN99_RS00510 WP_005586498.1 99281..99733(+) (pilA) [Aggregatibacter actinomycetemcomitans strain 23S]
ATGAAAATTCAATCCGCGTTTATTTCGCTAAAATTCGTTAAAAAAGGGTTTACATTAATCGAATTAATGATCGTCATCGC
TATTGTTGCTATTCTCGCCACCGTAGCCGTACCATCTTATCAAAATTACACTAAAAAAGCGGCGGTTTCCGAATTATTAC
AAGCCTCCGCCCCATTACGTTCCGAAGTGGAACTCTGTATTTATAACACCGGTAAGGCTGAAAATTGTAGCGGCGGACAA
AACGGCATTCAAGCAGATGTTGCAGATGCTTCGAAACAAAAATATGTGAAATCCACTGCGGTCAAAGGCGGCGTCATCAC
CGTTACCGGCAAAGGCAGTTTGGATAAAATCAGCTACACCCTTACCGCTTCGGGCACGGCTGCCAGCGGGGTCAGCTGGA
ACGCCGCCTGCGGCAATAATGCCGACATTTTCCCGGCAGGTTTCTGTTCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(14-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

62.319

92

0.573
  pilA Haemophilus influenzae Rd KW20

59.028

96

0.567
  pilA Glaesserella parasuis strain SC1401

53.846

95.333

0.513