Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   EKM72_RS08325 Genome accession   NZ_AP019305
Coordinates   1524527..1525639 (+) Length   370 a.a.
NCBI ID   WP_000775113.1    Uniprot ID   P68863
Organism   Staphylococcus aureus strain TUM9458     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1519527..1530639
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKM72_RS08300 rnpA 1519790..1520143 (-) 354 WP_001789343.1 ribonuclease P protein component -
  EKM72_RS08305 rpmH 1520264..1520401 (-) 138 WP_000240855.1 50S ribosomal protein L34 -
  EKM72_RS08310 dnaA 1521132..1522493 (+) 1362 WP_001290433.1 chromosomal replication initiator protein DnaA -
  EKM72_RS08315 dnaN 1522771..1523904 (+) 1134 WP_000969811.1 DNA polymerase III subunit beta -
  EKM72_RS08320 yaaA 1524285..1524530 (+) 246 WP_001789359.1 S4 domain-containing protein YaaA -
  EKM72_RS08325 recF 1524527..1525639 (+) 1113 WP_000775113.1 DNA replication/repair protein RecF Machinery gene
  EKM72_RS08330 gyrB 1525649..1527583 (+) 1935 WP_000255578.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  EKM72_RS08335 gyrA 1527620..1530289 (+) 2670 WP_000819059.1 DNA gyrase subunit A -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42415.43 Da        Isoelectric Point: 6.4362

>NTDB_id=61541 EKM72_RS08325 WP_000775113.1 1524527..1525639(+) (recF) [Staphylococcus aureus strain TUM9458]
MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIYTLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGT
MPLTMFITKKGKQVKVNHLEQSRLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDKEALSLNYLPSLKFDYAQNEAAR
LEEIMSILSDNMQREKERGISLFGPHRDDISFDVNGMDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLS
ELDDSRQTHLLSTIQHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=61541 EKM72_RS08325 WP_000775113.1 1524527..1525639(+) (recF) [Staphylococcus aureus strain TUM9458]
ATGAAGTTAAATACACTCCAATTAGAAAATTATCGTAACTATGATGAGGTTACGTTGAAATGTCATCCTGACGTGAATAT
CCTCATTGGAGAAAATGCACAAGGAAAGACAAATTTACTTGAATCAATTTATACCTTAGCTTTAGCAAAAAGTCATAGAA
CGAGTAATGATAAGGAACTCATACGTTTTAATGCTGATTATGCTAAAATAGAAGGTGAGCTTAGTTATAGACACGGCACG
ATGCCATTAACAATGTTTATAACTAAAAAAGGTAAACAAGTCAAAGTGAATCACTTAGAGCAAAGTCGTCTAACTCAATA
TATTGGACACCTCAATGTGGTTCTATTTGCGCCAGAAGATTTGAATATTGTAAAAGGCTCTCCTCAAATAAGACGACGCT
TTATAGATATGGAGTTGGGCCAAATTTCTGCTGTTTACTTAAATGATTTAGCTCAATACCAACGTATTTTAAAGCAAAAG
AATAATTACTTAAAGCAGTTACAATTAGGCCAAAAAAAGGACTTAACAATGTTGGAAGTATTAAATCAGCAGTTTGCTGA
ATATGCAATGAAAGTAACTGATAAACGTGCACATTTTATTCAAGAGCTAGAGTCGTTAGCTAAACCGATTCATGCTGGTA
TCACAAATGATAAAGAAGCGTTGTCGCTAAATTATTTACCTAGTCTTAAATTTGATTATGCTCAAAATGAAGCGGCACGA
CTTGAAGAAATTATGTCTATTCTTAGCGATAATATGCAAAGAGAAAAAGAACGAGGCATTAGCTTATTCGGACCACATCG
AGATGATATAAGTTTTGATGTGAATGGCATGGATGCTCAAACATATGGTTCTCAAGGACAGCAACGTACAACGGCTTTGT
CCATTAAATTAGCTGAAATTGAGTTAATGAATATCGAAGTTGGGGAATATCCCATCTTATTATTAGACGATGTACTCAGT
GAATTAGATGATTCGCGTCAAACGCATTTATTAAGTACGATTCAGCATAAAGTACAAACATTTGTCACTACGACATCTGT
AGATGGTATTGATCATGAAATCATGAATAACGCTAAATTGTATCGTATTAATCAAGGTGAAATTATAAAGTAA

Domains

Predicted by InterProScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(3-347)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P68863

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

58.649

100

0.586
  recF Streptococcus pneumoniae R6

49.33

100

0.497

Multiple sequence alignment