Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LIN93_RS00650 Genome accession   NZ_CP085091
Coordinates   127993..128445 (+) Length   150 a.a.
NCBI ID   WP_249852904.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans strain 30S     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 122993..133445
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIN93_RS00635 pnp 123950..126079 (-) 2130 WP_025298076.1 polyribonucleotide nucleotidyltransferase -
  LIN93_RS00640 nanQ 126373..126843 (+) 471 WP_005547536.1 N-acetylneuraminate anomerase -
  LIN93_RS00645 ampD 127317..127871 (-) 555 WP_005567973.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LIN93_RS00650 pilA 127993..128445 (+) 453 WP_249852904.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LIN93_RS00655 pilB 128472..129881 (+) 1410 WP_019518272.1 GspE/PulE family protein Machinery gene
  LIN93_RS00660 pilC 129874..131097 (+) 1224 WP_025298077.1 type II secretion system F family protein Machinery gene
  LIN93_RS00665 - 131094..131783 (+) 690 WP_005547547.1 A24 family peptidase -
  LIN93_RS00670 coaE 131833..132456 (+) 624 WP_005547548.1 dephospho-CoA kinase -
  LIN93_RS00675 yacG 132446..132658 (+) 213 WP_005567966.1 DNA gyrase inhibitor YacG -
  LIN93_RS00680 - 132658..132930 (+) 273 WP_005547552.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15531.92 Da        Isoelectric Point: 9.1384

>NTDB_id=615396 LIN93_RS00650 WP_249852904.1 127993..128445(+) (pilA) [Aggregatibacter actinomycetemcomitans strain 30S]
MKIQSAFISPKFVKKGFTLIELMIVIAIVAILATVAVPSYQNYTKKAAVSELLQASAPLRSEVELCIYNTGKAENCSGGQ
NGIQADVADASKQKYVKSTAVKGGVITVTGKGSLDKISYTLTASGTAASGVSWNAACGNNADIFPAGFCS

Nucleotide


Download         Length: 453 bp        

>NTDB_id=615396 LIN93_RS00650 WP_249852904.1 127993..128445(+) (pilA) [Aggregatibacter actinomycetemcomitans strain 30S]
ATGAAAATTCAATCCGCGTTTATTTCGCCAAAATTCGTTAAAAAAGGGTTTACATTAATCGAATTAATGATCGTCATCGC
TATTGTTGCTATTCTCGCCACCGTAGCCGTACCATCTTATCAAAATTACACTAAAAAAGCGGCGGTTTCCGAATTATTAC
AAGCCTCCGCCCCATTACGTTCCGAAGTGGAACTCTGTATTTATAACACCGGTAAGGCTGAAAATTGTAGCGGCGGACAA
AACGGCATTCAAGCAGATGTTGCAGATGCTTCGAAACAAAAATATGTGAAATCCACTGCGGTCAAAGGCGGCGTCATCAC
CGTTACCGGCAAAGGCAGTTTGGATAAAATCAGCTACACCCTTACCGCTTCGGGCACGGCTGCCAGCGGGGTCAGCTGGA
ACGCCGCCTGCGGCAATAATGCCGACATTTTCCCGGCAGGTTTCTGTTCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(14-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

59.722

96

0.573
  pilA Haemophilus influenzae Rd KW20

60.87

92

0.56
  pilA Glaesserella parasuis strain SC1401

55.797

92

0.513