Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LF296_RS15760 Genome accession   NZ_CP085083
Coordinates   3365472..3366278 (-) Length   268 a.a.
NCBI ID   WP_004775625.1    Uniprot ID   N8V219
Organism   Acinetobacter vivianii strain KJ-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3360472..3371278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LF296_RS15740 (LF296_15740) - 3361529..3362227 (-) 699 WP_047429260.1 YfgM family protein -
  LF296_RS15745 (LF296_15745) hisS 3362257..3363549 (-) 1293 WP_004775620.1 histidine--tRNA ligase -
  LF296_RS15750 (LF296_15750) ispG 3363546..3364661 (-) 1116 WP_005259644.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  LF296_RS15755 (LF296_15755) - 3364684..3365481 (-) 798 WP_004775623.1 helix-turn-helix domain-containing protein -
  LF296_RS15760 (LF296_15760) pilF 3365472..3366278 (-) 807 WP_004775625.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  LF296_RS15765 (LF296_15765) rlmN 3366296..3367531 (-) 1236 WP_272654910.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  LF296_RS15770 (LF296_15770) ndk 3367653..3368084 (-) 432 WP_004775629.1 nucleoside-diphosphate kinase -
  LF296_RS15775 (LF296_15775) iscX 3368193..3368393 (-) 201 WP_004775630.1 Fe-S cluster assembly protein IscX -
  LF296_RS15780 (LF296_15780) - 3368501..3369052 (-) 552 WP_004775631.1 phosphatase PAP2 family protein -
  LF296_RS15785 (LF296_15785) - 3369085..3370356 (-) 1272 WP_272656226.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 268 a.a.        Molecular weight: 30431.40 Da        Isoelectric Point: 9.7102

>NTDB_id=615124 LF296_RS15760 WP_004775625.1 3365472..3366278(-) (pilF) [Acinetobacter vivianii strain KJ-1]
MNTTRSKTIIATAVVFTLLGLTACQTPSTTLKKDPEKAVQVRTQLAAEYIRTRDLDSAKRSLDQALKLNSRDANANMMMG
ILLQQEGSAANMEKAESYFKRAISTEPDNAQARNNYGTYLYQQQRYNEAIEQFTRAGATLGYDQRFQSLENLGRIYLKLG
DVANAEKSFKQALQANRNATISMLELAEIFYLQQNNRDASQLYEQYVRNVGQKNQGARALWIGLRIARANADQLGMQVLV
NQLRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 807 bp        

>NTDB_id=615124 LF296_RS15760 WP_004775625.1 3365472..3366278(-) (pilF) [Acinetobacter vivianii strain KJ-1]
TTGAATACAACTCGATCAAAAACGATTATAGCGACAGCTGTTGTGTTTACTTTACTGGGCTTAACAGCTTGCCAGACGCC
ATCTACAACATTAAAAAAAGATCCGGAAAAAGCGGTGCAGGTTCGTACACAACTTGCGGCAGAATATATTCGTACACGTG
ATCTGGATTCAGCCAAGCGTTCGCTTGATCAGGCGTTAAAACTGAATTCCCGAGATGCCAACGCCAATATGATGATGGGC
ATCTTGCTGCAACAAGAAGGTAGTGCAGCAAATATGGAAAAAGCGGAGTCTTATTTTAAGCGCGCTATTTCTACCGAACC
AGATAACGCTCAGGCTCGTAATAATTACGGTACATATTTGTACCAGCAGCAACGCTATAATGAAGCCATTGAGCAATTTA
CCCGTGCGGGCGCAACCCTGGGTTATGATCAGCGTTTTCAGTCGCTGGAAAACCTGGGGCGAATCTATTTAAAACTTGGC
GATGTTGCGAATGCTGAAAAATCATTCAAACAGGCGCTACAAGCGAATCGAAACGCAACAATTTCAATGTTAGAGTTGGC
GGAAATTTTCTATTTGCAACAAAATAATAGAGATGCATCGCAACTATACGAACAATATGTTCGTAATGTAGGTCAGAAAA
ACCAGGGTGCCCGTGCGCTTTGGATTGGTTTACGCATTGCTCGCGCAAATGCGGATCAGTTAGGAATGCAAGTGTTAGTC
AATCAGTTACGCGCTTTATTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATG
GAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(110-177)

Tetratricopeptide repeat (TPR_8)

(41-70)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N8V219

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78.731

100

0.787
  pilF Acinetobacter baylyi ADP1

69.057

98.881

0.683