Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LG951_RS11460 Genome accession   NZ_CP085037
Coordinates   2249228..2250298 (-) Length   356 a.a.
NCBI ID   WP_081106456.1    Uniprot ID   -
Organism   Bacillus pumilus strain BIM B-171     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2244228..2255298
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG951_RS11415 (LG951_11415) sipW 2244321..2244893 (-) 573 WP_003217306.1 signal peptidase I SipW -
  LG951_RS11420 (LG951_11420) tapA 2244945..2245472 (-) 528 WP_106029150.1 amyloid fiber anchoring/assembly protein TapA -
  LG951_RS11425 (LG951_11425) - 2245772..2246050 (+) 279 WP_003217457.1 DUF3889 domain-containing protein -
  LG951_RS11430 (LG951_11430) - 2246084..2246278 (-) 195 WP_034620826.1 YqzE family protein -
  LG951_RS11435 (LG951_11435) - 2246338..2246721 (-) 384 WP_003217479.1 competence type IV pilus minor pilin ComGG -
  LG951_RS11440 (LG951_11440) comGF 2246718..2247248 (-) 531 WP_226803663.1 competence type IV pilus minor pilin ComGF -
  LG951_RS19905 comGE 2247157..2247474 (-) 318 WP_003217390.1 competence type IV pilus minor pilin ComGE -
  LG951_RS11445 (LG951_11445) comGD 2247458..2247904 (-) 447 WP_212045911.1 competence type IV pilus minor pilin ComGD -
  LG951_RS11450 (LG951_11450) comGC 2247897..2248190 (-) 294 WP_003217322.1 competence type IV pilus major pilin ComGC Machinery gene
  LG951_RS11455 (LG951_11455) comGB 2248207..2249247 (-) 1041 WP_212045909.1 competence type IV pilus assembly protein ComGB -
  LG951_RS11460 (LG951_11460) comGA 2249228..2250298 (-) 1071 WP_081106456.1 competence type IV pilus ATPase ComGA Machinery gene
  LG951_RS11465 (LG951_11465) - 2250476..2251348 (-) 873 WP_003217438.1 STAS domain-containing protein -
  LG951_RS11475 (LG951_11475) - 2251572..2251952 (-) 381 WP_003217382.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  LG951_RS11480 (LG951_11480) - 2252165..2252407 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  LG951_RS11485 (LG951_11485) - 2252652..2253290 (-) 639 WP_003217463.1 MBL fold metallo-hydrolase -
  LG951_RS11490 (LG951_11490) - 2253448..2253621 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  LG951_RS11495 (LG951_11495) - 2253845..2254159 (-) 315 WP_226803664.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40352.75 Da        Isoelectric Point: 9.2484

>NTDB_id=614825 LG951_RS11460 WP_081106456.1 2249228..2250298(-) (comGA) [Bacillus pumilus strain BIM B-171]
MYGIEYLGQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRRGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASTRLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGETCQLYCKLNRPVRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=614825 LG951_RS11460 WP_081106456.1 2249228..2250298(-) (comGA) [Bacillus pumilus strain BIM B-171]
TTGTATGGGATTGAATATTTGGGACAAGAGCTGCTAGAAGAAGCATGCCGCATGAGAGCATCTGATGTGCACATTGTTCC
AAAGGAAAAGGAAGCGTCGGTATCTTTCCGCGTAGACTCAGATTTAATTCAGCAGCGAACCATTGATAAAAAAAGCGGCG
AGCGGCTCATTGCTCATTTTAAATTTTTATCATCCATGGATATCGGAGAAAAACGGAGACCGCAAAATGGGTCATTAGCT
GTGATGCTAAGAAGGGGTCAGGTGTTTATTAGGATGTCTACCCTACCAACTGTAAATGATGAGAGCTTAGTGATTAGAAT
ATTGCCGCAGGATCATGTTCCAAAAATAAAACACCTGTCACTATTTCCGAAAGCATCCACCAGGTTATTATCATTTTTGA
ATCATTCGCATGGGCTCATTTTATTTACCGGTCCAACCAATTCAGGGAAAACAACAACCCTTTATTCATTGATCCAGTTT
GCAAAAAAGAATTTCAACAGAAATATTATTACACTTGAAGATCCTGTGGAAACGAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCCGGTATCACATATGCGGCAGGATTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCTCGAACGGCAATTAGAGCAGCTCTGACAGGCCATTTAGTGATGAGTACACTCCAT
GCGAAAAATGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTCGAACAAACGATGGTGGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAACATGCCAGCTTTACTGTAAATTAAATCGAC
CGGTCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAGGAGCTTGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACATTGCAAAGATTAATTCGTAAAGGAGTGGCACTAGGCTATTTATCGAAAAACACCTATCA
TCGCTGGGTTTATGAAGAAGCAAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.407

99.438

0.64