Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LG291_RS18775 Genome accession   NZ_CP084990
Coordinates   3692105..3693175 (-) Length   356 a.a.
NCBI ID   WP_394579912.1    Uniprot ID   -
Organism   Cytobacillus firmus strain WLG-456     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3687105..3698175
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG291_RS18730 (LG291_18575) - 3687599..3688393 (+) 795 WP_404355495.1 YqhG family protein -
  LG291_RS18735 (LG291_18580) - 3688437..3688622 (-) 186 WP_035329975.1 YqzE family protein -
  LG291_RS18740 (LG291_18585) - 3688701..3689198 (-) 498 WP_404355497.1 shikimate kinase -
  LG291_RS18745 (LG291_18590) comGG 3689211..3689594 (-) 384 WP_404355499.1 competence type IV pilus minor pilin ComGG -
  LG291_RS18750 (LG291_18595) comGF 3689584..3690000 (-) 417 WP_404355501.1 competence type IV pilus minor pilin ComGF -
  LG291_RS18755 (LG291_18600) - 3690014..3690343 (-) 330 WP_404355503.1 phosphatase -
  LG291_RS18760 (LG291_18605) comGD 3690327..3690767 (-) 441 WP_404355505.1 competence type IV pilus minor pilin ComGD -
  LG291_RS18765 (LG291_18610) comGC 3690764..3691087 (-) 324 WP_197247508.1 competence type IV pilus major pilin ComGC -
  LG291_RS18770 (LG291_18615) comGB 3691087..3692118 (-) 1032 WP_404355507.1 competence type IV pilus assembly protein ComGB -
  LG291_RS18775 (LG291_18620) comGA 3692105..3693175 (-) 1071 WP_394579912.1 competence type IV pilus ATPase ComGA Machinery gene
  LG291_RS18785 (LG291_18630) - 3693444..3693797 (-) 354 WP_048008265.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  LG291_RS18790 (LG291_18635) - 3694044..3694742 (+) 699 WP_163143401.1 metalloregulator ArsR/SmtB family transcription factor -
  LG291_RS18795 (LG291_18640) - 3694896..3695141 (+) 246 WP_009332938.1 DUF2626 domain-containing protein -
  LG291_RS18800 (LG291_18645) - 3695176..3696246 (-) 1071 WP_404359533.1 class I SAM-dependent methyltransferase -
  LG291_RS18805 (LG291_18650) - 3696451..3697086 (-) 636 WP_394579915.1 MBL fold metallo-hydrolase -
  LG291_RS18810 (LG291_18655) - 3697176..3697655 (+) 480 WP_404355510.1 hypothetical protein -
  LG291_RS18815 (LG291_18660) - 3697783..3697956 (+) 174 WP_048008269.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40141.36 Da        Isoelectric Point: 8.3356

>NTDB_id=614220 LG291_RS18775 WP_394579912.1 3692105..3693175(-) (comGA) [Cytobacillus firmus strain WLG-456]
MSIIERMAERIIKDAVRSHASDIHIIPRRKDTLIQLRFGSQLTPRLYLPKEECDRLISHFKFTASMDIGERRRPQSGAYS
LEVDGQMIGLRFSTLPSSHSESLVIRILPQQEQIPFYQISLFPDMTRKMLALLKHAHGLIIFTGPTGSGKTTTLYSLLNE
TSHMFHRNVITLEDPIEKENDLVLQVQVNEKAGVSYSAGLKAILRHDPDIIMVGEIRDAETAKIAVRAALTGHLVLSTMH
TRDAEGAVYRLAEFGVNLLEIEQTLVAVTAQRLVELNCPYCEKECSPFCYGYGRWKRASVFELLAGRALNASIKKARGES
SLVKYRTLKEVITKGIALGYIKETEYSRWVHDDGEA

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=614220 LG291_RS18775 WP_394579912.1 3692105..3693175(-) (comGA) [Cytobacillus firmus strain WLG-456]
TTGAGTATAATTGAAAGAATGGCAGAACGAATTATTAAAGACGCGGTCAGAAGCCATGCTTCCGACATCCACATCATTCC
CCGAAGAAAAGACACACTCATTCAGCTGAGGTTCGGCAGCCAATTGACCCCAAGGCTTTATCTTCCCAAAGAAGAGTGTG
ACAGATTGATTTCCCATTTTAAATTTACAGCATCGATGGATATTGGTGAAAGAAGAAGGCCGCAGAGCGGTGCATACTCA
CTTGAAGTAGACGGACAAATGATTGGCCTGCGCTTTTCAACCCTCCCATCAAGCCACAGCGAAAGCCTCGTTATCAGAAT
CCTTCCCCAGCAGGAACAAATTCCTTTTTACCAAATCAGCCTTTTTCCAGACATGACAAGAAAAATGCTGGCACTGCTGA
AGCATGCTCATGGTTTAATTATTTTCACTGGCCCGACCGGCAGCGGTAAAACCACAACTTTGTATTCCCTTCTGAATGAA
ACCTCCCATATGTTCCATCGCAATGTCATCACCCTGGAAGATCCCATTGAAAAAGAGAATGACTTAGTTCTTCAAGTCCA
AGTGAATGAGAAAGCGGGCGTGTCTTATTCTGCAGGCTTGAAAGCAATCCTTCGCCATGACCCCGATATCATTATGGTCG
GTGAAATCCGGGATGCGGAAACGGCGAAGATTGCAGTTAGAGCAGCTCTCACCGGTCACCTTGTTTTAAGCACCATGCAT
ACCCGGGATGCCGAGGGGGCTGTTTACCGTTTAGCTGAGTTTGGAGTGAACTTGCTGGAAATTGAGCAGACTTTGGTGGC
GGTCACTGCACAGCGCCTCGTCGAATTGAATTGTCCATATTGTGAAAAGGAATGCTCCCCATTCTGCTATGGATATGGCA
GGTGGAAAAGGGCGAGTGTGTTTGAATTGCTCGCAGGAAGGGCGCTCAATGCCTCCATAAAGAAGGCAAGAGGGGAAAGC
AGCCTTGTTAAGTATCGAACACTGAAGGAAGTCATAACTAAAGGGATTGCCCTAGGCTATATAAAGGAAACAGAGTACAG
CAGGTGGGTGCATGACGATGGAGAAGCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.618

100

0.556
  pilB Glaesserella parasuis strain SC1401

39.714

98.315

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.44

100

0.362