NTDB

Overview

Name	pilT	Type	Machinery gene
Locus tag	LGV73_RS18800	Genome accession	NZ_CP084879
Coordinates	3685949..3686929 (-)	Length	326 a.a.
NCBI ID	WP_001055627.1	Uniprot ID	A0A4P7TIP5
Organism	Shigella flexneri strain CMCC51574
Function	type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3680949..3691929

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LGV73_RS18770 (LGV73_18740)	-	3681612..3682109 (+)	498	WP_272606590.1	SprT family zinc-dependent metalloprotease	-
LGV73_RS18775 (LGV73_18745)	endA	3682204..3682911 (+)	708	WP_000286517.1	deoxyribonuclease I	-
LGV73_RS18780 (LGV73_18750)	rsmE	3682991..3683722 (+)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
LGV73_RS18785 (LGV73_18755)	gshB	3683735..3684685 (+)	951	WP_000593267.1	glutathione synthase	-
LGV73_RS18790 (LGV73_18760)	-	3684794..3685357 (+)	564	WP_001053178.1	YqgE/AlgH family protein	-
LGV73_RS18795 (LGV73_18765)	ruvX	3685357..3685773 (+)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
LGV73_RS18800 (LGV73_18770)	pilT	3685949..3686929 (-)	981	WP_001055627.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LGV73_RS18805 (LGV73_18775)	yggS	3686947..3687651 (+)	705	WP_000997799.1	pyridoxal phosphate homeostasis protein	-
LGV73_RS18810 (LGV73_18780)	yggT	3687669..3688235 (+)	567	WP_001094818.1	osmotic shock tolerance protein YggT	-
LGV73_RS18815 (LGV73_18785)	yggU	3688232..3688522 (+)	291	WP_001277222.1	DUF167 family protein YggU	-
LGV73_RS18820 (LGV73_18790)	-	3688530..3689123 (+)	594	WP_001174735.1	XTP/dITP diphosphatase	-
LGV73_RS18825 (LGV73_18795)	hemW	3689116..3690252 (+)	1137	WP_000239924.1	radical SAM family heme chaperone HemW	-
LGV73_RS18830 (LGV73_18800)	-	3690566..3691529 (+)	964	Protein_3682	TRAP transporter substrate-binding protein	-

Sequence

Protein

Download Length: 326 a.a. Molecular weight: 35942.12 Da Isoelectric Point: 5.7980

                                >NTDB_id=613773 LGV73_RS18800 WP_001055627.1 3685949..3686929(-) (pilT) [Shigella flexneri strain CMCC51574]

MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG

SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE

YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS

FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR

VGEGRL

Nucleotide

Download Length: 981 bp

                                >NTDB_id=613773 LGV73_RS18800 WP_001055627.1 3685949..3686929(-) (pilT) [Shigella flexneri strain CMCC51574]

ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG

ATGGCGCATTCGTGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG

ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC

AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG

TGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCTACGGGGAGTGGCAAAT

CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA

TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG

GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTGACGG

CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA

TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTTGCGGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT

GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA

AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG

GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A4P7TIP5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baylyi ADP1	46.789	100	0.469
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	46.177	100	0.463
pilT	Legionella pneumophila strain ERS1305867	45.26	100	0.454
pilT	Legionella pneumophila strain Lp02	45.26	100	0.454
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	39.514	100	0.399
pilU	Pseudomonas stutzeri DSM 10701	37.576	100	0.38
pilU	Acinetobacter baylyi ADP1	36.957	98.773	0.365
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362

Detailed information NTG0613773