Detailed information NTG0613483

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: NP435_RS08595
• Genome accession: NZ_CP101937
• Coordinates: 1649127..1650116 (+)
• Length: 329 a.a.
• NCBI ID: WP_003232026.1
• Uniprot ID: P51835
• Organism: Bacillus subtilis strain RUB331
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1644127..1655116

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NP435_RS08580 (NP435_08580)	acpP	1644324..1644557 (+)	234	WP_003154310.1	acyl carrier protein	-
NP435_RS08585 (NP435_08585)	rncS	1644697..1645446 (+)	750	WP_003232030.1	ribonuclease III	-
NP435_RS08590 (NP435_08590)	smc	1645547..1649107 (+)	3561	WP_003232028.1	chromosome segregation protein SMC	-
NP435_RS08595 (NP435_08595)	pilA	1649127..1650116 (+)	990	WP_003232026.1	signal recognition particle-docking protein FtsY	Machinery gene
NP435_RS08600 (NP435_08600)	sivC	1650153..1650638 (-)	486	WP_003245834.1	Ig-like domain-containing protein	-
NP435_RS08605 (NP435_08605)	srpM	1650815..1651147 (+)	333	WP_003238590.1	putative DNA-binding protein	-
NP435_RS08610 (NP435_08610)	ffh	1651161..1652501 (+)	1341	WP_003232020.1	signal recognition particle protein	-
NP435_RS08615 (NP435_08615)	rpsP	1652607..1652879 (+)	273	WP_003220815.1	30S ribosomal protein S16	-
NP435_RS08620 (NP435_08620)	khpA	1652879..1653124 (+)	246	WP_003232017.1	KH domain-containing protein	-
NP435_RS08625 (NP435_08625)	ylqD	1653246..1653632 (+)	387	WP_003232015.1	YlqD family protein	-
NP435_RS08630 (NP435_08630)	rimM	1653637..1654161 (+)	525	WP_003232013.1	ribosome maturation factor RimM	-
NP435_RS08635 (NP435_08635)	trmD	1654158..1654889 (+)	732	WP_003232011.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-

Sequence

Protein

• Length: 329 a.a.
• Molecular weight: 36342.52 Da
• Isoelectric Point: 5.0164

>NTDB_id=613483 NP435_RS08595 WP_003232026.1 1649127..1650116(+) (pilA) [Bacillus subtilis strain RUB331]
MSFFKKLKEKITKQTDSVSEKFKDGLEKTRNSFQNKVNDLVSRYRKVDEDFFEELEEVLISADVGFTTVMELIDELKKEV
KRRNIQDPKEVQSVISEKLVEIYNSGDEQISELNIQDGRLNVILLVGVNGVGKTTTIGKLAHKMKQEGKSVVLAAGDTFR
AGAIEQLEVWGERTGVPVIKQTAGSDPAAVIYDAVHAAKARNADVLICDTAGRLQNKVNLMKELEKVKRVIEREVPEAPH
EVLLALDATTGQNAMAQAKEFSKATNVTGIALTKLDGTAKGGIVLAIRNELHIPVKLVGLGEKVDDLQEFDPESYVYGLF
SDLVEKADD

Nucleotide

• Length: 990 bp

>NTDB_id=613483 NP435_RS08595 WP_003232026.1 1649127..1650116(+) (pilA) [Bacillus subtilis strain RUB331]
ATGAGCTTTTTTAAAAAATTAAAAGAGAAAATCACAAAACAGACAGATTCCGTATCTGAAAAGTTTAAGGATGGCCTTGA
AAAAACAAGAAACTCCTTTCAAAACAAAGTGAATGATCTTGTATCCCGTTACCGTAAAGTGGATGAGGATTTCTTCGAAG
AGCTTGAAGAGGTTCTTATCAGCGCGGATGTCGGTTTTACAACCGTTATGGAATTAATAGATGAGCTGAAAAAAGAAGTC
AAACGCAGAAATATTCAAGATCCAAAGGAAGTCCAGTCAGTGATTTCTGAGAAACTGGTCGAGATTTATAACAGCGGAGA
TGAGCAAATTTCAGAACTGAACATCCAGGATGGGCGTTTAAACGTAATCCTTCTGGTAGGTGTAAACGGCGTCGGGAAAA
CAACAACGATCGGAAAGCTTGCTCATAAAATGAAACAAGAAGGAAAATCTGTTGTACTTGCCGCCGGAGACACTTTTAGA
GCGGGAGCCATTGAACAGCTGGAAGTATGGGGAGAGCGTACAGGAGTGCCTGTCATTAAGCAGACGGCAGGAAGCGATCC
GGCGGCTGTCATCTACGATGCTGTTCATGCTGCGAAAGCAAGAAATGCCGATGTATTAATTTGTGATACGGCAGGGCGTC
TCCAAAACAAAGTAAATCTCATGAAAGAGCTTGAAAAAGTAAAACGTGTTATCGAAAGAGAAGTTCCTGAAGCTCCGCAT
GAGGTGCTGCTTGCCCTTGATGCCACGACCGGCCAAAATGCAATGGCTCAGGCAAAAGAATTCTCTAAAGCAACAAATGT
TACCGGCATTGCTTTAACGAAGCTTGACGGTACGGCAAAAGGCGGTATCGTCCTTGCGATTCGCAACGAGCTTCACATCC
CGGTTAAACTAGTCGGTTTAGGAGAAAAAGTTGATGACCTTCAGGAATTTGATCCAGAATCCTATGTGTACGGACTCTTT
TCAGATTTAGTGGAAAAAGCCGACGATTAA

Domains

Predicted by InterProScan.

• SRP54-type protein, GTPase domain (SRP54) (121-320)

• SRP54-type protein, helical bundle domain (SRP54_N) (34-99)

3D structure

• AlphaFold DB: P51835

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	43.709	91.793	0.401