Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NP442_RS18450 Genome accession   NZ_CP101922
Coordinates   3747281..3747721 (+) Length   146 a.a.
NCBI ID   WP_001727303.1    Uniprot ID   -
Organism   Escherichia coli strain JCC-EE 7     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3742281..3752721
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NP442_RS18430 (NP442_18425) aroP 3743279..3744652 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  NP442_RS18435 (NP442_18430) ampE 3744695..3745549 (-) 855 WP_000172014.1 beta-lactamase regulator AmpE -
  NP442_RS18440 (NP442_18435) ampD 3745546..3746097 (-) 552 WP_098717141.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NP442_RS18445 (NP442_18440) nadC 3746185..3747078 (+) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NP442_RS18450 (NP442_18445) pilA 3747281..3747721 (+) 441 WP_001727303.1 prepilin peptidase-dependent pilin Machinery gene
  NP442_RS18455 (NP442_18450) gspE 3747731..3749117 (+) 1387 Protein_3624 type II secretion system protein GspE -
  NP442_RS18460 (NP442_18455) hofC 3749107..3750309 (+) 1203 WP_021566125.1 protein transport protein HofC -
  NP442_RS18465 (NP442_18460) guaC 3750344..3751387 (-) 1044 WP_001217338.1 GMP reductase -
  NP442_RS18470 (NP442_18465) - 3751543..3751587 (-) 45 WP_120795372.1 protein YacM -
  NP442_RS18475 (NP442_18470) coaE 3751612..3752232 (+) 621 WP_001269532.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15649.81 Da        Isoelectric Point: 4.3938

>NTDB_id=612708 NP442_RS18450 WP_001727303.1 3747281..3747721(+) (pilA) [Escherichia coli strain JCC-EE 7]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVVKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=612708 NP442_RS18450 WP_001727303.1 3747281..3747721(+) (pilA) [Escherichia coli strain JCC-EE 7]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGTAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363