Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   LG401_RS05155 Genome accession   NZ_CP084711
Coordinates   1053105..1056809 (+) Length   1234 a.a.
NCBI ID   WP_216454729.1    Uniprot ID   -
Organism   Bacillus pumilus strain AR03     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1048105..1061809
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG401_RS05140 (LG401_05140) - 1048397..1048807 (+) 411 WP_034663477.1 DoxX family protein -
  LG401_RS05145 (LG401_05145) - 1049004..1049450 (-) 447 WP_212040938.1 ferritin-like domain-containing protein -
  LG401_RS05150 (LG401_05150) addB 1049618..1053127 (+) 3510 WP_060595950.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  LG401_RS05155 (LG401_05155) addA 1053105..1056809 (+) 3705 WP_216454729.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  LG401_RS05160 (LG401_05160) sbcD 1056836..1058011 (+) 1176 WP_060595952.1 exonuclease subunit SbcD -
  LG401_RS05165 (LG401_05165) sbcC 1058008..1061403 (+) 3396 WP_106071581.1 exonuclease subunit SbcC -
  LG401_RS05170 (LG401_05170) - 1061423..1061722 (+) 300 WP_060595954.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142314.33 Da        Isoelectric Point: 5.0164

>NTDB_id=612608 LG401_RS05155 WP_216454729.1 1053105..1056809(+) (addA) [Bacillus pumilus strain AR03]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEHPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPLPEQWMRAFLSLYDVDAQTKVEELPFYPYIKEDLSLVLRSCQELLERA
LSLSKEPGGPAPRAANFIDDLEQVNELICHQDDFEELYELLPNVNFKRLKTCKGDEYDPVLLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVDLVIQFGERFERAKQEKSIVDFSDLEHYCLRILAEQDAEGHLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHSAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKHFQPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLDQQEAETGEEREELETVQFEARIIAKK
IRELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPIVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFQLLRKWRDFSINHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEEWLLPDFERYQSKTYLDFIGPALIRHQAMSSVLEETGDVVLSHPSTFTISFTQASDL
LKEDMSLEKKQQDEVVQALMDGLPVEGYGDADEKVAERLSWKYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSIRKAR
ATLYDRPAFMKKKTLTAAEQGTAMHTVMQHITLPSEEPYDESRIRHLLESLQQRDLLTDEQVQSIDQEGIAAFFSTSIGQ
KLLKADWVKREVSFSMVLPVKEVYSHIDTEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTGGLDAAEPILKKRYE
TQIALYAKAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=612608 LG401_RS05155 WP_216454729.1 1053105..1056809(+) (addA) [Bacillus pumilus strain AR03]
ATGCAAATACCAAAACCGAATAACAGTACGTGGACGGATGACCAATGGGAAGCCATCGTTTCAGAAGGACAAGATATCTT
GGTTGCGGCGGCGGCAGGGTCAGGAAAAACAGCTGTTTTGGTTGAACGGCTGATTCGAAAAATGACCCGGCCTGAACATC
CAGTTGATGTGGACCGTCTGCTCGTTGTGACTTTCACAAATGCATCTGCGGCAGAGATGAAGCATCGGATTACAGAAGCA
CTTGAAAAAGAATTAGCCAAAAACCCTGGGTCCCTTCATATGAGAAGGCAGCTGTCCCTCATGAATCGTGCCAATATTTC
TACCTTGCACTCCTTTTGCTTGCAGGTTTTGCGCACCTTTTACTATGAAATTGATCTTGACCCAGGCTTTCGTTTGGCAG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTCGAGGATGAATATAAGGCTGGAAAGCCATCA
TTCTTTGAACTGGTTGATCGTTACACTTCTGACCGCCATGACTTAGATTTGCAATGGCTTGTGAAGAGAATTTATGAGTT
TTCTAGGTCTCATCCTTTACCTGAGCAATGGATGAGGGCATTCCTTTCACTTTATGATGTAGATGCACAAACAAAAGTAG
AAGAATTACCGTTTTATCCATATATCAAAGAAGATCTTTCTCTCGTTCTCCGGAGCTGCCAGGAGCTGCTTGAACGAGCT
CTTTCGCTATCAAAAGAGCCGGGCGGCCCAGCACCGAGAGCAGCGAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCTGTCATCAAGATGATTTTGAGGAGCTTTACGAGCTTTTGCCGAACGTCAATTTTAAAAGGCTCAAAACGTGCAAAG
GGGATGAATATGACCCTGTTTTGTTAGAAAAGGCCACAGATGCACGTAATCAAGCAAAAAAACAACTAGAAAAGCTAAAA
GATGAATACTTTATGCGCAGTCCTGCCCAGCATTTAAAAAGCTTAGCTGAAATGAAGCCAGTTGTGGAGACGCTTGTAGA
TCTTGTGATCCAATTTGGTGAGCGTTTCGAGAGAGCGAAGCAGGAAAAGTCAATTGTCGACTTTTCGGATTTAGAGCACT
ATTGCTTACGCATTTTAGCGGAGCAGGATGCAGAAGGACATTTGATCGAAACAGAAGCTGCCAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATACCAGGATACAAACCTCGTACAAGAAACGATTTTAAAGCTTGTGTCTAAAGGGGA
ACATTCTGCAGAGGGCAATCTGTTTATGGTTGGTGATGTAAAGCAGTCCATTTATCGTTTTAGACTGGCTGAGCCCATGC
TCTTTTTAAACAAATATAAACACTTTCAACCAGACGGCAAAGAAACAGGGAAGAGAATTGATTTAAATAAAAACTTCCGA
AGCCGTTCAGATGTTTTAGATAGTACGAACTTTTTGTTCAAGCAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCAAAGATACAACGACAGAAATGCTCCTTGTCCACTTAG
ATCAGCAGGAAGCAGAAACCGGCGAAGAGCGAGAAGAACTTGAGACCGTCCAATTTGAAGCGAGAATCATTGCTAAAAAA
ATAAGAGAATTGGTGGAGCAGCCATTTCAAGTGTACGATGCAAAACAGCAAATGACCCGTAACTTGCAGTATCGTGACAT
TGTCATTTTGCTTCGGTCTATGCCGTGGGCCCCGCAAATGATGGAAGAATTAAAGAAGCAGGGAATCCCAGTATATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTCATTCTTTCTTTGTTAAAAGTCATCGATAATCCGTATCAG
GATATTCCGCTTGCAGCTGTTTTAAGATCACCTATTGTTCATTTAGATGAAAATGAAATGGCACTTATTCGAACGAGTGA
TAAAAAAGGCACTTATTATGATGCTGTAAAGGCATTCATGAGTGTGACGCATTCTGATCACCCTACATGCAAAAAATTAG
AGCGATTTTTCCAATTGCTGCGTAAGTGGCGTGATTTCTCCATCAATCATTCGGTGGCAGAATTAATTTGGGAAGTATAC
CGAGATACGCAGTATCTTGATTATGTTGGCGGGATGCCTGGAGGTAAGCAGCGGCAGGCGAATCTTCGTGCCCTTTATGA
TCGAGCCAAGCAATATGAAAAAGCTGCCTTTAGAGGGCTATTTCGATTCCTTCGTTTTATTGAAAGAATGCAGGAGCGTG
GAGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTGCGCATGATGACGATCCACAGCAGTAAAGGA
TTAGAATTTCCAGTCGTCTTTACTGCAGGACTCGGCAGGAACTTTAATATGATGGACTTAAACCAGTCCTACTTACTTGA
TAAAGAGCTAGGCTTTGGAAGCAAGTATATCCATCCAGAGCTGAGAATTAGTTATGCGACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGTTATTATCTGAAGAGCTGAGGGTCTTATATGTGGCGCTCACAAGGGCTAAGGAAAAATTA
TTTCTGGTCGGCTCTGTCAAAAATCAGGTGAAAGCATTAAGCAAATGGCAAAATGCCGCGACTGGAGAAGAGTGGCTGCT
CCCTGATTTTGAACGTTATCAATCGAAAACATACTTAGATTTTATTGGCCCTGCCCTTATCCGTCATCAAGCGATGTCAT
CTGTTTTAGAAGAAACAGGCGATGTTGTTCTTTCGCATCCGTCTACATTCACTATCTCGTTTACGCAAGCTTCTGATCTT
CTAAAGGAAGATATGTCACTTGAAAAGAAGCAGCAAGACGAGGTAGTACAAGCATTGATGGATGGTCTGCCTGTTGAAGG
ATATGGTGATGCTGATGAGAAGGTGGCAGAGAGACTTTCATGGAAATATCCATATCTAGCGGCCTCGCAAGTCGGCACAA
AGCAGTCTGTTTCCGAGATAAAAAGGATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTATTCGAAAAGCTCGT
GCAACATTGTATGACCGGCCGGCTTTCATGAAGAAAAAAACACTCACTGCCGCAGAACAAGGCACCGCAATGCATACAGT
CATGCAGCATATCACGCTTCCTTCTGAGGAGCCCTATGATGAATCACGTATAAGGCACCTGCTCGAATCCTTACAGCAGC
GAGACTTGCTAACAGATGAGCAAGTCCAGTCAATTGATCAAGAAGGAATTGCTGCTTTCTTCTCTACTTCAATTGGTCAA
AAACTGCTGAAAGCGGACTGGGTAAAACGAGAGGTATCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGTCATAT
TGATACTGAGGGAGAGCCTGTTCTGATTCAAGGAATGATTGACTGTTTATTTGAAACAGATGGAAAGCTATATTTACTAG
ATTATAAAACGGATAGAGTGCAAGGCAGATATACAGGTGGACTTGACGCTGCTGAACCTATCTTGAAAAAGCGCTATGAA
ACCCAAATAGCGTTATATGCAAAAGCAGTAGAGCGTTTGACAAACAGAACATTAGAGGAGAAAATTCTCTATTTCTTCGA
TGGGAATTTAGAAATCTCTTTATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1226)

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.424

100

0.665