Detailed information NTG0612542

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: LGQ02_RS10535
• Genome accession: NZ_CP084703
• Coordinates: 2130973..2134701 (+)
• Length: 1242 a.a.
• NCBI ID: WP_226518102.1
• Uniprot ID: -
• Organism: Bacillus shivajii strain JCM 32183
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 2125973..2139701

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LGQ02_RS10525 (LGQ02_10525)	-	2126856..2127005 (+)	150	WP_226518100.1	hypothetical protein	-
LGQ02_RS10530 (LGQ02_10530)	addB	2127474..2130983 (+)	3510	WP_226518101.1	helicase-exonuclease AddAB subunit AddB	-
LGQ02_RS10535 (LGQ02_10535)	addA	2130973..2134701 (+)	3729	WP_226518102.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
LGQ02_RS10540 (LGQ02_10540)	-	2134704..2135876 (+)	1173	WP_226518103.1	exonuclease SbcCD subunit D	-
LGQ02_RS10545 (LGQ02_10545)	-	2135873..2139256 (+)	3384	WP_226518104.1	AAA family ATPase	-

Sequence

Protein

• Length: 1242 a.a.
• Molecular weight: 143776.10 Da
• Isoelectric Point: 4.6881

>NTDB_id=612542 LGQ02_RS10535 WP_226518102.1 2130973..2134701(+) (addA) [Bacillus shivajii strain JCM 32183]
MNVNPKPLDVTWTDDQWKAIDARGNNILVAAAAGSGKTAVLVERIIRTIVDHQADVDRLLIVTFTNAAAAEMRTRIGEAI
EKELKKDPGSLHLRRQLTLLNRANISTLHSFCMKVLKKYYFEVEIDPNFRLLDETEGELIREEVIDDLFEEEYGKENNHE
FYAVVDRYSGDRSDDALRNLIRKLYNFSRSNPNPNQWLDELVHAYNLDDVGSMEELIWTKELFFDIHQQLEGAEAWIQKG
MELTKEPAGPYKYAENFEEDLKVLYTLRQCKTWSEMYEAFQQAKFGRLKSIPKKDHVDENLKERAKKYRDTTKKVVEAIQ
KDYFSKEPEAFMHELKEMAPTVETTVRLVKEFAKRYMDAKKEKAVLDFNDLEHLCLQVLLDADGNELKPSKAALEFKDHF
VEVLIDEYQDTNLVQETILYLLAKENNRFMVGDVKQSIYRFRLAEPSLFLDKYKTYVKSGDGDGWRIDLSKNFRSRSEIL
DATNFIFRQIMDESVGEIPYDTDAELHLGNLDYPEQEGLEAELAVINKGDPLEVKDPQDESQSLEEDLETSQLETRYIIQ
QVKKLVKEQHLVYDKSIKGQRPITYRDIVILMRSMPWASTMMEECKKEGIPVYADLSTGYFSAIEVQIVMSLLKIIDNPY
QDIPLASVLRSPIVGFNEQELAHIRMMKKEVSYYDALKTTAEESPEDEIKEKAADFLEKLTNWRTNARSGALFELIWQIY
RETGYFDYVGGMPGGKQRQANLRALYDRARSYESTSFRGLFRFLRFIERMQERGDDLGTARALGEQEDVVRIMTIHKSKG
LEFPVVFVAGMNKKFNLQDMSASYLLHKELGFGTKFIDPKLRVAYPSIPQLAIKKRMHLESLAEEMRVLYVALTRAKEKL
YLVGTVQNAEKSLENWQDLLGEDDWLLPAMDRQRGKTFMDWVGPAVLRHGSSSCYHGPGGVICENEVAEDASKWSMDIIE
RDTLQVEEKTEVEIQEDKLHKLYYLEPVEGAANESWVDEKLNWQYDRIQASIHRSKQTVSEMKRELEDEYSDVMFGVSKS
FQSAYADRPQFLQEKGLSSTEIGTVMHTVMQHVPLTEPPTTEKIDRLLVGMYEKELLTRDQVEAVDVNHVIEFFNTDIGE
RMLNAKWVEREIPFSYGLPAVEAYQTWEEDEHTDGEKVLIQGVIDCIFEDENGKIILVDYKTDKITGKYKSFEDAKEALL
GRYELQLSIYSGALEEIWKRDIDESILYFFDGGHVLNLEEGV

Nucleotide

• Length: 3729 bp

>NTDB_id=612542 LGQ02_RS10535 WP_226518102.1 2130973..2134701(+) (addA) [Bacillus shivajii strain JCM 32183]
ATGAACGTTAATCCTAAACCTCTTGATGTGACATGGACCGATGATCAATGGAAAGCAATCGATGCAAGAGGAAACAACAT
TTTAGTTGCAGCCGCAGCGGGAAGCGGGAAGACAGCCGTTTTAGTAGAAAGAATTATCCGTACGATCGTGGATCATCAAG
CTGATGTCGACCGCTTACTAATCGTGACGTTCACAAACGCAGCAGCGGCTGAAATGAGGACAAGAATTGGTGAAGCGATT
GAAAAAGAATTAAAAAAAGATCCCGGTTCCCTACACCTGCGTAGACAATTAACGTTATTAAACCGTGCGAACATCTCAAC
CCTTCATTCGTTTTGTATGAAAGTGTTGAAGAAGTATTACTTTGAAGTTGAAATTGATCCGAACTTTCGTCTCCTAGATG
AAACAGAAGGGGAACTCATCCGTGAAGAAGTGATCGACGACCTTTTTGAAGAGGAGTACGGAAAGGAAAATAATCACGAG
TTTTATGCTGTTGTTGATCGGTATAGTGGAGACCGAAGTGATGATGCGCTTCGAAATTTAATTCGAAAGCTATATAACTT
CTCTCGCTCCAACCCGAATCCAAATCAATGGTTAGATGAGCTCGTTCATGCATACAACTTAGATGATGTAGGAAGCATGG
AAGAGTTAATTTGGACAAAAGAGCTATTTTTTGACATCCATCAGCAATTAGAAGGTGCGGAAGCATGGATTCAAAAAGGG
ATGGAACTGACGAAAGAGCCAGCTGGACCGTATAAGTACGCGGAAAACTTTGAAGAAGATTTAAAAGTTCTTTATACGCT
CCGCCAATGTAAAACGTGGAGCGAAATGTATGAAGCGTTCCAACAAGCGAAGTTTGGCCGCTTAAAATCGATTCCGAAAA
AAGACCATGTTGATGAGAACCTAAAGGAACGTGCGAAAAAATATCGCGATACGACGAAAAAGGTCGTTGAAGCGATTCAA
AAAGATTACTTTTCAAAAGAACCTGAAGCGTTCATGCATGAATTAAAAGAAATGGCACCAACCGTTGAAACGACTGTTCG
GTTAGTGAAAGAGTTCGCTAAAAGGTATATGGACGCGAAAAAAGAGAAAGCTGTCCTCGATTTTAACGACCTTGAGCACC
TTTGTTTACAAGTGTTGCTCGACGCTGATGGTAATGAATTAAAACCGTCAAAAGCCGCGTTAGAATTTAAAGATCACTTT
GTTGAAGTGTTGATTGACGAGTATCAAGATACGAACCTCGTCCAAGAAACGATTCTTTATTTATTAGCAAAAGAAAATAA
CCGCTTTATGGTTGGGGACGTAAAGCAAAGTATTTATCGTTTCCGTTTAGCAGAACCATCGTTATTCCTAGATAAGTACA
AAACGTACGTAAAGTCTGGTGATGGAGATGGCTGGCGTATTGACTTATCGAAAAACTTTCGAAGCCGTAGCGAAATATTA
GATGCAACAAACTTTATTTTTCGCCAAATCATGGATGAATCTGTAGGAGAAATCCCATATGATACCGATGCTGAGCTTCA
TTTAGGGAACTTAGATTATCCAGAGCAAGAAGGGCTTGAAGCAGAGCTTGCCGTCATTAATAAAGGGGATCCGTTAGAAG
TAAAAGACCCACAAGATGAGTCACAATCATTAGAAGAAGATTTAGAAACATCCCAGCTTGAAACGAGATACATCATTCAA
CAAGTGAAAAAGCTCGTGAAAGAACAACATCTTGTTTATGACAAAAGTATAAAAGGGCAGCGCCCAATCACGTACCGTGA
CATCGTTATTTTAATGCGTTCAATGCCATGGGCCTCGACCATGATGGAAGAGTGTAAAAAAGAAGGCATTCCTGTATACG
CGGATCTATCAACAGGTTACTTTAGTGCGATTGAAGTGCAAATTGTCATGTCGCTTTTAAAAATCATTGATAATCCGTAT
CAAGATATTCCGCTCGCATCTGTATTACGCTCACCAATTGTCGGCTTTAATGAGCAAGAGCTTGCGCACATTCGTATGAT
GAAGAAAGAAGTATCCTATTATGATGCGTTAAAAACGACAGCGGAAGAATCGCCAGAAGACGAGATCAAAGAAAAGGCAG
CGGACTTCTTAGAAAAATTAACGAACTGGCGAACGAATGCTAGATCAGGTGCACTGTTTGAACTCATTTGGCAAATTTAC
CGTGAAACAGGTTACTTTGACTATGTCGGTGGGATGCCTGGAGGTAAGCAGCGTCAAGCGAACCTCCGTGCGTTATATGA
CCGTGCTCGATCCTATGAATCGACGTCGTTCCGCGGTTTGTTCCGTTTCCTTCGTTTTATTGAGCGAATGCAAGAGCGTG
GTGATGATTTAGGTACGGCACGAGCATTAGGGGAACAAGAAGATGTCGTGCGCATTATGACGATTCATAAAAGTAAAGGT
CTGGAATTCCCCGTCGTGTTTGTTGCGGGAATGAACAAAAAGTTTAATTTACAAGACATGAGTGCGTCTTATTTACTTCA
TAAAGAGTTAGGTTTCGGCACAAAGTTCATCGATCCAAAGTTACGTGTCGCTTACCCAAGCATTCCGCAACTTGCGATTA
AAAAGCGTATGCATTTAGAAAGCTTAGCAGAAGAAATGCGTGTCTTATATGTAGCGCTCACTCGTGCGAAAGAGAAGCTC
TATTTAGTCGGAACGGTTCAAAATGCTGAAAAGTCATTAGAGAACTGGCAAGACTTACTCGGGGAAGATGACTGGCTTTT
ACCTGCAATGGACAGGCAGCGTGGTAAAACATTTATGGACTGGGTTGGACCTGCTGTTTTAAGGCACGGCTCATCAAGCT
GTTATCACGGCCCAGGTGGTGTCATCTGTGAAAATGAGGTGGCTGAAGACGCGTCAAAATGGTCGATGGATATTATTGAA
CGAGACACGTTACAAGTCGAAGAAAAAACAGAAGTAGAAATACAAGAAGATAAGCTTCATAAGTTATATTATCTAGAACC
TGTTGAAGGTGCAGCAAATGAAAGCTGGGTTGATGAAAAGCTAAATTGGCAATATGACCGTATTCAAGCGTCGATTCACC
GTTCAAAACAAACGGTTAGTGAAATGAAGCGTGAGTTAGAAGATGAGTATAGTGATGTCATGTTCGGCGTTTCGAAATCG
TTTCAATCTGCGTATGCGGACAGACCTCAGTTTTTACAAGAAAAAGGTTTAAGTTCAACAGAAATTGGAACCGTCATGCA
TACTGTTATGCAGCATGTCCCATTAACTGAGCCACCGACCACGGAAAAAATCGATCGCTTATTAGTTGGGATGTATGAAA
AAGAATTGTTAACGAGAGATCAAGTCGAAGCTGTTGATGTGAACCATGTCATTGAGTTTTTCAATACAGATATCGGCGAG
AGAATGTTAAATGCGAAATGGGTGGAGCGTGAAATTCCATTTAGTTACGGACTTCCGGCTGTTGAAGCTTACCAAACGTG
GGAAGAAGACGAGCATACTGATGGGGAGAAAGTTCTCATCCAAGGGGTCATTGACTGTATTTTTGAAGATGAAAATGGCA
AGATCATCTTAGTTGATTACAAAACAGACAAGATTACTGGGAAGTATAAAAGTTTTGAAGATGCGAAGGAAGCACTTCTC
GGCAGATATGAACTCCAACTATCGATTTATTCCGGGGCGCTTGAAGAAATTTGGAAGCGTGATATCGATGAATCGATTCT
TTATTTCTTTGATGGTGGCCATGTCTTGAATTTGGAAGAAGGTGTGTAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (467-883)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (12-456)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1047-1229)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	51.597	100	0.52