Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   LDB25_RS18530 Genome accession   NZ_CP084695
Coordinates   3680777..3682162 (-) Length   461 a.a.
NCBI ID   WP_014419012.1    Uniprot ID   I2CAS2
Organism   Bacillus velezensis strain DJB5     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3675777..3687162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDB25_RS18495 (LDB25_18420) flgL 3675952..3676869 (-) 918 WP_014419007.1 flagellar hook-associated protein FlgL -
  LDB25_RS18500 (LDB25_18425) flgK 3676881..3678398 (-) 1518 WP_014419008.1 flagellar hook-associated protein FlgK -
  LDB25_RS18505 (LDB25_18430) - 3678414..3678896 (-) 483 WP_003151394.1 flagellar protein FlgN -
  LDB25_RS18510 (LDB25_18435) flgM 3678911..3679177 (-) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  LDB25_RS18515 (LDB25_18440) - 3679247..3679666 (-) 420 WP_014419009.1 TIGR03826 family flagellar region protein -
  LDB25_RS18520 (LDB25_18445) comFC 3679740..3680429 (-) 690 WP_197535984.1 ComF family protein Machinery gene
  LDB25_RS18525 (LDB25_18450) - 3680435..3680719 (-) 285 WP_014419011.1 late competence development ComFB family protein -
  LDB25_RS18530 (LDB25_18455) comFA 3680777..3682162 (-) 1386 WP_014419012.1 DEAD/DEAH box helicase Machinery gene
  LDB25_RS18535 (LDB25_18460) - 3682270..3683118 (-) 849 WP_003151387.1 DegV family protein -
  LDB25_RS18540 (LDB25_18465) degU 3683216..3683905 (-) 690 WP_111927758.1 two-component system response regulator DegU Regulator
  LDB25_RS18545 (LDB25_18470) degS 3683982..3685145 (-) 1164 WP_007407484.1 two-component sensor histidine kinase DegS Regulator
  LDB25_RS18550 (LDB25_18475) - 3685368..3686015 (+) 648 WP_007614107.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52599.04 Da        Isoelectric Point: 10.2804

>NTDB_id=612495 LDB25_RS18530 WP_014419012.1 3680777..3682162(-) (comFA) [Bacillus velezensis strain DJB5]
MSEGASEFSEVRDFFYGRHLLRSEIPFSDQRIKRLTEKEYITAEPSIIRRKNRYVCQRCGQSDQASFAAFWAPSAKRQIT
YCRACVMMGRADELTSLYSWNQAPENSWEPVKLSWEGTLTDGQKRAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLNKAFEKVEVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKKAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
RQKLQLQLPVFLFVPSVHVLKKTTDYFRKLNIRAEGVHAEDTFRKDKVKRFRDGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVMFHFGITNGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=612495 LDB25_RS18530 WP_014419012.1 3680777..3682162(-) (comFA) [Bacillus velezensis strain DJB5]
ATGTCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTAAGATCTGAGATACCGTT
TTCAGACCAGCGCATCAAGCGTTTAACAGAAAAAGAATATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCAGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAAAGGCAGATTACC
TATTGCCGCGCCTGTGTCATGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAGCCCCTGAAAACAG
CTGGGAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCGGGCGGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCGGGTATCGAGTTT
GCGCTGAATCACGGTTTGCGCGTTTGTGTCGCAACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAATAA
AGCATTTGAGAAAGTCGAAGTTTCTGCTTTGTACGGCGGCAGCGAGGACAAAGGCCGCTTGACTCCGCTGATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGATTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCGACTCCTTCAGA
CACATTAAAAAAGAAGGCTGAAGCCGGTTTGCTGAAAAGCGTCCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACGGATTGGGTC
CGCCAAAAACTTCAATTACAGCTGCCGGTTTTCTTATTCGTCCCGTCTGTACACGTATTGAAAAAGACAACGGATTATTT
TCGGAAATTAAACATACGGGCAGAAGGCGTACATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGATTCAGAGACG
GCCGGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATTACAAACGGAATGAAAAAAGCAAAAAAACATATAGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(118-266)

Helicase conserved C-terminal domain (Helicase_C)

(330-421)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2CAS2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

62.391

99.783

0.623