Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NP447_RS02570 Genome accession   NZ_CP101920
Coordinates   537269..538702 (-) Length   477 a.a.
NCBI ID   WP_000809047.1    Uniprot ID   -
Organism   Escherichia coli strain QA-1986 630     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 532269..543702
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NP447_RS02535 (NP447_02535) elbB 532960..533613 (+) 654 WP_001307414.1 isoprenoid biosynthesis glyoxalase ElbB -
  NP447_RS02540 (NP447_02540) mtgA 533610..534338 (+) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  NP447_RS02545 (NP447_02545) yrbL 534335..534967 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  NP447_RS02550 (NP447_02550) npr 535181..535453 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  NP447_RS02555 (NP447_02555) rapZ 535450..536304 (-) 855 WP_000243741.1 RNase adapter RapZ -
  NP447_RS02560 (NP447_02560) ptsN 536350..536841 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NP447_RS02565 (NP447_02565) hpf 536959..537246 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  NP447_RS02570 (NP447_02570) rpoN/rpoN1 537269..538702 (-) 1434 WP_000809047.1 RNA polymerase factor sigma-54 Machinery gene
  NP447_RS02575 (NP447_02575) lptB 538750..539475 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  NP447_RS02580 (NP447_02580) lptA 539482..540039 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  NP447_RS02585 (NP447_02585) lptC 540008..540583 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  NP447_RS02590 (NP447_02590) kdsC 540580..541146 (-) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  NP447_RS02595 (NP447_02595) kdsD 541167..542153 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  NP447_RS02600 (NP447_02600) yrbG 542167..543144 (-) 978 WP_000922872.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54051.83 Da        Isoelectric Point: 4.3447

>NTDB_id=612495 NP447_RS02570 WP_000809047.1 537269..538702(-) (rpoN/rpoN1) [Escherichia coli strain QA-1986 630]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDDDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=612495 NP447_RS02570 WP_000809047.1 537269..538702(-) (rpoN/rpoN1) [Escherichia coli strain QA-1986 630]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCAACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGTTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGATTACATTGACGA
CGAGCTGCCTGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTAACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGATGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCACTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTACTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465