Detailed information NTG0612480

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LDB25_RS13680
• Genome accession: NZ_CP084695
• Coordinates: 2742069..2742683 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain DJB5
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2737069..2747683

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LDB25_RS13650 (LDB25_13615)	rpsT	2737372..2737638 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
LDB25_RS13655 (LDB25_13620)	holA	2737655..2738696 (-)	1042	Protein_2646	DNA polymerase III subunit delta	-
LDB25_RS13660 (LDB25_13625)	-	2738736..2738887 (-)	152	Protein_2647	hypothetical protein	-
LDB25_RS13665 (LDB25_13630)	-	2738928..2739062 (+)	135	WP_003152870.1	YqzM family protein	-
LDB25_RS13670 (LDB25_13635)	comEC	2739081..2741432 (-)	2352	WP_014418418.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LDB25_RS13675 (LDB25_13640)	-	2741433..2742002 (-)	570	WP_003152868.1	ComE operon protein 2	-
LDB25_RS13680 (LDB25_13645)	comEA	2742069..2742683 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
LDB25_RS13685 (LDB25_13650)	comER	2742742..2743563 (+)	822	WP_014418420.1	late competence protein ComER	-
LDB25_RS13690 (LDB25_13655)	-	2743632..2744369 (-)	738	WP_014418421.1	class I SAM-dependent DNA methyltransferase	-
LDB25_RS13695 (LDB25_13660)	rsfS	2744366..2744722 (-)	357	WP_007408260.1	ribosome silencing factor	-
LDB25_RS13700 (LDB25_13665)	yqeK	2744740..2745300 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
LDB25_RS13705 (LDB25_13670)	-	2745290..2745859 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
LDB25_RS13710 (LDB25_13675)	yhbY	2745870..2746160 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
LDB25_RS13715 (LDB25_13680)	aroE	2746154..2746996 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=612480 LDB25_RS13680 WP_014418419.1 2742069..2742683(-) (comEA) [Bacillus velezensis strain DJB5]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=612480 LDB25_RS13680 WP_014418419.1 2742069..2742683(-) (comEA) [Bacillus velezensis strain DJB5]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363