Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LGL97_RS12290 Genome accession   NZ_CP084655
Coordinates   2747565..2748581 (+) Length   338 a.a.
NCBI ID   WP_225583478.1    Uniprot ID   -
Organism   Pectobacterium brasiliense strain SR10     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2742565..2753581
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LGL97_RS12260 (LGL97_12260) hemW 2743279..2744421 (-) 1143 WP_225583479.1 radical SAM family heme chaperone HemW -
  LGL97_RS12265 (LGL97_12265) rdgB 2744414..2745007 (-) 594 WP_010279113.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  LGL97_RS12270 (LGL97_12270) yggU 2745025..2745315 (-) 291 WP_010279110.1 DUF167 family protein YggU -
  LGL97_RS12275 (LGL97_12275) - 2745312..2745866 (-) 555 WP_010279107.1 YggT family protein -
  LGL97_RS12280 (LGL97_12280) proC 2745915..2746736 (-) 822 WP_225583620.1 pyrroline-5-carboxylate reductase -
  LGL97_RS12285 (LGL97_12285) - 2746831..2747544 (-) 714 WP_010279099.1 YggS family pyridoxal phosphate-dependent enzyme -
  LGL97_RS12290 (LGL97_12290) pilT 2747565..2748581 (+) 1017 WP_225583478.1 type IV pilus twitching motility protein PilT Machinery gene
  LGL97_RS12295 (LGL97_12295) - 2748550..2750382 (-) 1833 WP_052902890.1 diguanylate cyclase -
  LGL97_RS12300 (LGL97_12300) - 2750913..2752484 (-) 1572 WP_225583477.1 methyl-accepting chemotaxis protein -
  LGL97_RS12305 (LGL97_12305) nsrR 2752795..2753220 (+) 426 WP_010279087.1 nitric oxide-sensing transcriptional repressor NsrR -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36681.88 Da        Isoelectric Point: 6.6533

>NTDB_id=612329 LGL97_RS12290 WP_225583478.1 2747565..2748581(+) (pilT) [Pectobacterium brasiliense strain SR10]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTSDQVAQWCDAWLEPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSAQPSLTTLQAPPVLSTLLEKPNGLILITGATGSGKSTTLAAMLGALNDSSDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQKLLPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=612329 LGL97_RS12290 WP_225583478.1 2747565..2748581(+) (pilT) [Pectobacterium brasiliense strain SR10]
ATGGAATTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCAGT
GCTGCGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGTCGGATCAGGTGGCACAATGGTGCGATG
CTTGGCTCGAACCCGCTCAGCGCGAGCAACTACGGCAGGCTGGGCAGGTAGATGGGGCATTAATGCTGCCTGATGGACAA
CGCCTGCGGGTGAATGTATTTCGCCAGCGGGAAGGGCTGTCTGCGGCGCTGCGTATTATTCCGTCCGCTCAGCCTTCATT
GACGACGCTACAGGCACCACCCGTTCTCTCGACGCTGCTGGAGAAACCGAACGGACTGATCCTGATTACCGGTGCGACGG
GGAGCGGTAAATCCACGACGCTGGCGGCCATGCTAGGGGCGTTAAACGACAGTAGCGATCGCCATGTGATTACGCTGGAG
GACCCAATCGAGTTTATCCATGCCAGCCGACGTTGCCTGATTCAACAGCGGGAGATTGGTGCGCACAGTGCGTCGTTTGC
GCAGGCGCTACGGGCCGCATTACGGGAAGATCCCGATGTCATTCTGCTGGGGGAATTGCGTGATACGGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTATCCACGCTGCATACACGTAGTGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCAGGTGAAGAGAAAGCCTATGTTCGTAGCCAGTTAGCTACTTGTTTGCAGGCGGTAGTGACGCA
AAAACTGCTGCCCGCTGCGCAAGGTGGCCGAATCGCACTTTATGAAGTGCTGACGGCGACTGCGGCGGTCAGCAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTTCCCGGCCTGATTCAAACCGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAACGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.515

97.633

0.503
  pilT Vibrio cholerae strain A1552

51.515

97.633

0.503
  pilT Neisseria meningitidis 8013

50.301

98.225

0.494
  pilT Neisseria gonorrhoeae MS11

50

98.225

0.491
  pilT Pseudomonas stutzeri DSM 10701

49.245

97.929

0.482
  pilT Pseudomonas aeruginosa PAK

48.943

97.929

0.479
  pilT Legionella pneumophila strain Lp02

48.943

97.929

0.479
  pilT Legionella pneumophila strain ERS1305867

48.943

97.929

0.479
  pilT Acinetobacter baylyi ADP1

48.943

97.929

0.479
  pilT Acinetobacter nosocomialis M2

48.64

97.929

0.476
  pilT Acinetobacter baumannii D1279779

48.64

97.929

0.476
  pilT Acinetobacter baumannii strain A118

48.64

97.929

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.136

99.704

0.42
  pilU Vibrio cholerae strain A1552

39.067

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

39.64

98.521

0.391
  pilU Acinetobacter baylyi ADP1

38.509

95.266

0.367