Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LGL97_RS05675 Genome accession   NZ_CP084655
Coordinates   1288169..1289551 (+) Length   460 a.a.
NCBI ID   WP_225582622.1    Uniprot ID   -
Organism   Pectobacterium brasiliense strain SR10     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1283169..1294551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LGL97_RS05655 (LGL97_05655) prfC 1283453..1285042 (-) 1590 WP_010284696.1 peptide chain release factor 3 -
  LGL97_RS05660 (LGL97_05660) - 1285334..1286269 (+) 936 WP_225582621.1 hypothetical protein -
  LGL97_RS05665 (LGL97_05665) - 1286323..1287018 (-) 696 WP_052902212.1 YtjB family periplasmic protein -
  LGL97_RS05670 (LGL97_05670) serB 1287175..1288152 (+) 978 WP_010284684.1 phosphoserine phosphatase -
  LGL97_RS05675 (LGL97_05675) radA 1288169..1289551 (+) 1383 WP_225582622.1 DNA repair protein RadA Machinery gene
  LGL97_RS05680 (LGL97_05680) - 1289600..1289998 (-) 399 WP_225582623.1 VOC family protein -
  LGL97_RS05685 (LGL97_05685) - 1290117..1291043 (+) 927 WP_225582624.1 LysR substrate-binding domain-containing protein -
  LGL97_RS05690 (LGL97_05690) nadR 1291127..1292380 (+) 1254 WP_225582625.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  LGL97_RS05695 (LGL97_05695) - 1292377..1293024 (-) 648 WP_225582626.1 5-formyltetrahydrofolate cyclo-ligase -
  LGL97_RS05705 (LGL97_05705) zapA 1293322..1293651 (-) 330 WP_225582627.1 cell division protein ZapA -
  LGL97_RS05710 (LGL97_05710) - 1293844..1294431 (+) 588 WP_010284662.1 YecA family protein -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49558.11 Da        Isoelectric Point: 7.4218

>NTDB_id=612319 LGL97_RS05675 WP_225582622.1 1288169..1289551(+) (radA) [Pectobacterium brasiliense strain SR10]
MAKAVKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLASASTSRSDRLTGYAGESAGVSRVQKLSEISLEALPRFSTG
FQEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLSENMKTLYVTGEESLQQVAMRAHRLNLPTQNLNMLSETSIE
QICLIAEQEQPKLMVIDSIQVMHLADIQSSPGSVAQVRETAAYLTRLAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREISNPSAIFLSRGDEITSGSSVMVVWEGTRPLLVEIQALVDQ
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALLLSLVSSFRDRPLPQDLVIFGEV
GLAGEIRPVPSGQERITEAAKHGFKRAIVPHANMPKKAPASMQVFGVKKLADALAILDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=612319 LGL97_RS05675 WP_225582622.1 1288169..1289551(+) (radA) [Pectobacterium brasiliense strain SR10]
GTGGCAAAAGCCGTCAAACGGGCGTTTGTATGTAATGAATGCGGGGCTGACTATCCCCGCTGGCAAGGGCAGTGCAGCGC
CTGCCATGCCTGGAACACCATTACCGAAGTCCGTCTGGCCTCGGCGTCAACATCACGTTCCGACCGTCTCACCGGCTATG
CGGGCGAAAGCGCTGGCGTCAGCCGGGTACAAAAGCTCTCGGAAATCAGTCTCGAAGCCCTGCCGCGCTTTTCCACCGGC
TTTCAGGAGTTTGACCGCGTTCTGGGCGGCGGCGTCGTTCCTGGCAGCGCGATTCTGATCGGCGGTAACCCCGGCGCGGG
TAAAAGTACCTTACTGCTGCAAACGCTCTGCAAGCTGTCAGAGAACATGAAAACCCTGTACGTCACCGGGGAAGAATCAT
TACAGCAGGTGGCGATGCGGGCGCATCGTCTCAACCTGCCGACCCAGAATCTTAATATGCTGTCGGAAACCAGCATCGAA
CAGATTTGCCTGATTGCCGAGCAGGAACAGCCGAAGCTGATGGTGATCGACTCCATTCAGGTGATGCATCTCGCTGATAT
TCAATCGTCTCCCGGTAGCGTAGCGCAGGTGCGTGAAACAGCCGCCTACCTGACGCGCTTAGCCAAAACACGCGGCGTTG
CTATCGTGATGGTCGGCCATGTCACCAAAGACGGTTCGCTGGCCGGGCCGAAAGTGTTAGAACACTGCATCGACTGCTCC
GTGCTGTTGGATGGCGATGCCGATTCACGCTTCCGCACCCTGCGCAGCCATAAAAACCGCTTCGGTGCCGTTAACGAGCT
GGGCGTGTTCGCGATGACGGAACAGGGTCTACGCGAGATCAGCAATCCGTCGGCAATTTTCCTCAGTCGCGGTGACGAAA
TCACGTCCGGCAGTTCGGTCATGGTGGTGTGGGAAGGTACGCGTCCGCTGCTGGTCGAAATTCAGGCTCTGGTGGATCAA
TCGATGATGGCTAACCCGCGTCGCGTGGCAGTCGGGCTGGAGCAAAACCGATTAGCGATCCTGCTGGCGGTATTACATCG
CCACGGCGGCTTGCAGATGTCCGATCAGGATGTGTTCGTGAATGTCGTCGGCGGCGTTAAAGTCACCGAAACCAGCGCCG
ACCTGGCGCTGCTATTATCCCTGGTCTCCAGCTTCCGCGACCGCCCACTGCCGCAGGATCTTGTCATCTTCGGTGAAGTC
GGTCTGGCGGGCGAAATCCGCCCGGTTCCGAGCGGCCAAGAGCGGATTACCGAAGCCGCTAAGCACGGCTTCAAACGCGC
CATCGTTCCTCATGCCAATATGCCGAAGAAAGCCCCTGCCAGCATGCAGGTGTTCGGCGTGAAAAAGCTAGCCGACGCGC
TGGCAATCCTCGACGATCTCTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.261

100

0.483
  radA Streptococcus mitis NCTC 12261

43.202

99.13

0.428
  radA Streptococcus pneumoniae Rx1

43.202

99.13

0.428
  radA Streptococcus pneumoniae D39

43.202

99.13

0.428
  radA Streptococcus pneumoniae R6

43.202

99.13

0.428
  radA Streptococcus pneumoniae TIGR4

43.202

99.13

0.428
  radA Streptococcus mitis SK321

43.202

99.13

0.428