Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NP446_RS01945 Genome accession   NZ_CP101912
Coordinates   427209..428561 (+) Length   450 a.a.
NCBI ID   WP_016852368.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain ATCC 27853     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 422209..433561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NP446_RS01925 (NP446_01925) - 423046..423612 (-) 567 WP_003117889.1 hypothetical protein -
  NP446_RS01930 (NP446_01930) rsmD 423615..424211 (-) 597 WP_009875718.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  NP446_RS01935 (NP446_01935) - 424211..425698 (-) 1488 WP_016852367.1 pitrilysin family protein -
  NP446_RS01940 (NP446_01940) - 425691..427088 (-) 1398 WP_003084498.1 pitrilysin family protein -
  NP446_RS01945 (NP446_01945) pilA 427209..428561 (+) 1353 WP_016852368.1 signal recognition particle-docking protein FtsY Machinery gene
  NP446_RS01950 (NP446_01950) ftsE 428558..429229 (+) 672 WP_003084505.1 cell division ATP-binding protein FtsE -
  NP446_RS01955 (NP446_01955) ftsX 429229..430236 (+) 1008 WP_003084508.1 permease-like cell division protein FtsX -
  NP446_RS01960 (NP446_01960) rpoH 430350..431204 (+) 855 WP_003084510.1 RNA polymerase sigma factor RpoH -
  NP446_RS01965 (NP446_01965) - 431269..431874 (+) 606 WP_003084511.1 hypothetical protein -
  NP446_RS01970 (NP446_01970) mtgA 431879..432610 (-) 732 WP_003100972.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  NP446_RS01975 (NP446_01975) - 432647..433024 (+) 378 WP_003084513.1 DUF423 domain-containing protein -
  NP446_RS01980 (NP446_01980) thiS 433127..433327 (+) 201 WP_003084514.1 sulfur carrier protein ThiS -

Sequence

Protein


Download         Length: 450 a.a.        Molecular weight: 47814.23 Da        Isoelectric Point: 4.6111

>NTDB_id=612301 NP446_RS01945 WP_016852368.1 427209..428561(+) (pilA) [Pseudomonas aeruginosa strain ATCC 27853]
MFGSNDDKKAPQGGEKKGLFGWWRKKPQAGEQPADQPVEPVSETAAAEQRAPADDVAQSLTEQPGRQQPSAAEPAEPAPV
AEAPLASGEPASAEEHSPRPEAPVAQPEPILAAEPEPEPEPEPEPVAPLAAAPAVSEPATRPGFFARLRQGLSKTSASIG
EGMASLFLGRKEIDDDLLDDIETRLLTADVGVEATTLIVQNLTKRVARKELADSGALYKALQEELASLLRPVEQPLQVDV
AREPYVILVVGVNGVGKTTTIGKLAKKLQLEGKKVMLAAGDTFRAAAVEQLQVWGERNRIPVIAQHTGADSASVIFDAVQ
AAKARGIDVLIADTAGRLHTKDNLMEELKKVRRVIGKLDETAPHEVLLVLDAGTGQNAINQAKQFNLAVELTGLALTKLD
GTAKGGVIFALAKQFGLPIRYIGVGEGIDDLRTFEADAFVQALFAERENA

Nucleotide


Download         Length: 1353 bp        

>NTDB_id=612301 NP446_RS01945 WP_016852368.1 427209..428561(+) (pilA) [Pseudomonas aeruginosa strain ATCC 27853]
ATGTTTGGTTCCAACGATGACAAGAAGGCCCCGCAGGGCGGGGAAAAGAAAGGTCTGTTCGGCTGGTGGCGGAAAAAGCC
GCAAGCCGGCGAGCAGCCTGCCGATCAGCCTGTCGAGCCGGTCTCCGAGACAGCGGCCGCCGAGCAGCGCGCGCCTGCCG
ATGACGTGGCGCAATCCCTGACAGAACAGCCAGGTCGGCAGCAGCCCTCCGCTGCCGAGCCGGCCGAGCCGGCGCCGGTT
GCCGAAGCACCGCTCGCCAGTGGCGAGCCAGCGTCGGCCGAGGAGCATTCGCCCCGGCCCGAGGCGCCCGTCGCGCAACC
CGAACCTATCCTGGCTGCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGTTGCGCCGCTGGCCGCCGCTCCGG
CAGTCAGCGAGCCGGCGACCAGGCCGGGCTTCTTCGCCCGCCTCCGGCAGGGACTGTCCAAGACCAGCGCCAGCATCGGC
GAAGGCATGGCCAGCCTGTTCCTCGGCCGCAAGGAAATCGACGACGACCTGCTCGACGACATCGAGACGCGCCTGCTGAC
CGCCGACGTCGGCGTCGAGGCCACCACCCTGATCGTGCAGAACCTGACCAAGCGCGTGGCGCGCAAGGAGCTGGCCGACA
GCGGCGCGCTGTACAAGGCGCTGCAGGAAGAGCTGGCCAGCCTGCTGCGCCCGGTGGAGCAGCCGTTGCAGGTCGACGTC
GCGCGCGAGCCCTATGTGATCCTGGTGGTCGGGGTGAACGGCGTGGGCAAGACCACCACCATCGGCAAGCTGGCGAAGAA
GCTGCAACTCGAGGGCAAGAAGGTCATGCTGGCCGCCGGCGACACCTTCCGCGCTGCCGCCGTGGAGCAGTTGCAGGTCT
GGGGCGAGCGCAACCGTATCCCGGTGATCGCCCAGCACACCGGCGCCGACTCCGCCTCGGTGATCTTCGACGCGGTGCAG
GCCGCCAAGGCCCGCGGCATCGACGTGCTGATCGCCGATACCGCCGGACGCCTGCATACCAAGGACAACCTGATGGAGGA
GCTGAAGAAGGTGCGCCGGGTGATCGGCAAGCTGGACGAGACGGCGCCCCATGAGGTCCTGCTGGTGCTCGACGCCGGCA
CCGGGCAGAACGCCATCAACCAGGCCAAGCAGTTCAACCTCGCGGTGGAGCTGACCGGCCTGGCCCTGACCAAACTGGAC
GGCACCGCCAAGGGCGGGGTGATCTTCGCCCTGGCCAAGCAGTTCGGCCTGCCGATCCGCTATATCGGTGTCGGCGAGGG
GATCGACGATCTCCGTACCTTCGAGGCCGACGCCTTCGTCCAGGCACTCTTCGCAGAGCGGGAGAACGCATGA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(245-444)

SRP54-type protein, helical bundle domain (SRP54_N)

(160-225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.489

97.333

0.462