Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NP444_RS01875 Genome accession   NZ_CP101911
Coordinates   410417..411775 (+) Length   452 a.a.
NCBI ID   WP_014602384.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NWRC-1223     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 405417..416775
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NP444_RS01855 (NP444_01855) - 406254..406820 (-) 567 WP_003117889.1 hypothetical protein -
  NP444_RS01860 (NP444_01860) rsmD 406823..407419 (-) 597 WP_003102864.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  NP444_RS01865 (NP444_01865) - 407419..408906 (-) 1488 WP_003102862.1 pitrilysin family protein -
  NP444_RS01870 (NP444_01870) - 408899..410296 (-) 1398 WP_003084498.1 pitrilysin family protein -
  NP444_RS01875 (NP444_01875) pilA 410417..411775 (+) 1359 WP_014602384.1 signal recognition particle-docking protein FtsY Machinery gene
  NP444_RS01880 (NP444_01880) ftsE 411772..412443 (+) 672 WP_003084505.1 cell division ATP-binding protein FtsE -
  NP444_RS01885 (NP444_01885) ftsX 412443..413450 (+) 1008 WP_003084508.1 permease-like cell division protein FtsX -
  NP444_RS01890 (NP444_01890) rpoH 413564..414418 (+) 855 WP_003084510.1 RNA polymerase sigma factor RpoH -
  NP444_RS01895 (NP444_01895) - 414483..415088 (+) 606 WP_003084511.1 hypothetical protein -
  NP444_RS01900 (NP444_01900) mtgA 415093..415824 (-) 732 WP_003100972.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  NP444_RS01905 (NP444_01905) - 415861..416238 (+) 378 WP_003084513.1 DUF423 domain-containing protein -
  NP444_RS01910 (NP444_01910) thiS 416341..416541 (+) 201 WP_003084514.1 sulfur carrier protein ThiS -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 48098.50 Da        Isoelectric Point: 4.5525

>NTDB_id=612200 NP444_RS01875 WP_014602384.1 410417..411775(+) (pilA) [Pseudomonas aeruginosa strain NWRC-1223]
MFGSNDDKKAPQGGEKKGLFGWWRKKPQAGEQPADQPVEPVSETAAAEQRAPADDVAQSLTEQPGRQQPSAAEPAEPAPV
AEAPLASDEPASAEEHSPRPEAPVAQPEPILAAEPEPEPEPEPEPEPVAPLAAAPAVSEPATRPGFFARLRQGLSKTSAS
IGEGMASLFLGRKEIDDDLLDDIETRLLTADVGVEATTLIVQNLTKRVARKELADSGALYKALQEELASLLRPVEQPLQV
DVAREPYVILVVGVNGVGKTTTIGKLAKKLQLEGKKVMLAAGDTFRAAAVEQLQVWGERNRIPVIAQHTGADSASVIFDA
VQAAKARGIDVLIADTAGRLHTKDNLMEELKKVRRVIGKLDETAPHEVLLVLDAGTGQNAINQAKQFNLAVELTGLALTK
LDGTAKGGVIFALAKQFGLPIRYIGVGEGIDDLRTFEADAFVQALFAERENA

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=612200 NP444_RS01875 WP_014602384.1 410417..411775(+) (pilA) [Pseudomonas aeruginosa strain NWRC-1223]
ATGTTTGGTTCCAACGACGACAAGAAGGCCCCGCAGGGCGGGGAAAAGAAAGGTCTGTTCGGCTGGTGGCGGAAAAAGCC
GCAAGCCGGCGAGCAGCCTGCCGATCAGCCTGTCGAGCCGGTCTCCGAGACAGCGGCCGCCGAGCAGCGCGCGCCTGCCG
ATGACGTGGCGCAATCCCTGACAGAACAGCCAGGTCGGCAGCAGCCCTCCGCTGCCGAGCCGGCCGAGCCGGCGCCGGTT
GCCGAAGCACCGCTCGCCAGTGACGAGCCAGCGTCGGCCGAGGAGCATTCGCCCCGGCCCGAGGCGCCCGTCGCGCAACC
CGAGCCTATCCTGGCTGCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGTTGCGCCTCTGGCCGCCG
CTCCGGCAGTCAGCGAGCCGGCGACCAGGCCGGGCTTCTTCGCCCGCCTCCGGCAGGGACTGTCCAAGACCAGCGCCAGC
ATCGGCGAAGGCATGGCCAGCCTGTTCCTCGGCCGCAAGGAAATCGACGACGACCTGCTCGACGACATCGAGACGCGCCT
GCTGACCGCCGACGTCGGCGTCGAGGCCACCACCCTGATCGTGCAGAACCTGACCAAGCGCGTGGCGCGCAAGGAGCTGG
CCGACAGCGGCGCGCTGTACAAGGCGCTGCAGGAAGAGCTGGCCAGCCTGCTGCGCCCGGTGGAGCAGCCGTTGCAGGTC
GACGTCGCGCGCGAGCCCTATGTGATCCTGGTGGTCGGGGTGAACGGCGTGGGCAAGACCACCACCATCGGCAAGCTGGC
GAAGAAGCTGCAACTCGAGGGCAAGAAGGTCATGCTGGCCGCCGGCGACACCTTCCGCGCCGCCGCCGTGGAGCAGTTGC
AGGTCTGGGGCGAGCGCAACCGCATCCCGGTGATCGCCCAGCACACCGGCGCCGACTCCGCCTCGGTGATCTTCGACGCG
GTGCAGGCCGCCAAGGCCCGCGGCATCGACGTGCTGATCGCCGATACCGCCGGACGCCTGCATACCAAGGACAACCTGAT
GGAGGAGCTGAAGAAGGTGCGCCGGGTGATCGGCAAGCTGGACGAGACGGCGCCCCATGAGGTCCTGCTGGTGCTCGACG
CCGGCACCGGGCAGAACGCCATCAACCAGGCCAAGCAGTTCAACCTCGCGGTGGAGCTGACCGGCCTGGCCCTGACCAAA
CTGGACGGCACCGCCAAGGGCGGGGTGATCTTCGCCCTGGCCAAGCAGTTCGGCCTGCCGATCCGCTATATCGGTGTCGG
CGAGGGGATCGACGATCTCCGTACCTTCGAGGCCGACGCCTTCGTCCAGGCACTCTTCGCAGAGCGGGAGAACGCATGA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(162-227)

SRP54-type protein, GTPase domain (SRP54)

(247-446)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.235

96.018

0.454