Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   DCL27_RS14110 Genome accession   NZ_CP084506
Coordinates   3048165..3049634 (+) Length   489 a.a.
NCBI ID   WP_005281701.1    Uniprot ID   D4F169
Organism   Edwardsiella tarda ATCC 15947 = NBRC 105688 strain ATCC 15947     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3043165..3054634
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DCL27_RS14080 (DCL27_14090) - 3043595..3044584 (+) 990 WP_005281708.1 calcium/sodium antiporter -
  DCL27_RS14085 (DCL27_14095) kdsD 3044637..3045623 (+) 987 WP_005281707.1 arabinose-5-phosphate isomerase KdsD -
  DCL27_RS14090 (DCL27_14100) kdsC 3045639..3046205 (+) 567 WP_005297337.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  DCL27_RS14095 (DCL27_14105) lptC 3046202..3046774 (+) 573 WP_035597408.1 LPS export ABC transporter periplasmic protein LptC -
  DCL27_RS14100 (DCL27_14110) lptA 3046749..3047306 (+) 558 WP_005281704.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  DCL27_RS14105 (DCL27_14115) lptB 3047313..3048038 (+) 726 WP_005281703.1 LPS export ABC transporter ATP-binding protein -
  DCL27_RS14110 (DCL27_14120) rpoN/rpoN1 3048165..3049634 (+) 1470 WP_005281701.1 RNA polymerase factor sigma-54 Machinery gene
  DCL27_RS14115 (DCL27_14125) hpf 3049656..3049943 (+) 288 WP_024524583.1 ribosome hibernation promoting factor -
  DCL27_RS14120 (DCL27_14130) ptsN 3050061..3050525 (+) 465 WP_005281697.1 PTS IIA-like nitrogen regulatory protein PtsN -
  DCL27_RS14125 (DCL27_14135) rapZ 3050633..3051484 (+) 852 WP_005281696.1 RNase adapter RapZ -
  DCL27_RS14130 (DCL27_14140) npr 3051492..3051764 (+) 273 WP_005281695.1 PTS phosphocarrier protein NPr -
  DCL27_RS14135 (DCL27_14145) rnk 3051941..3052351 (+) 411 WP_035597411.1 nucleoside diphosphate kinase regulator -
  DCL27_RS14140 (DCL27_14150) - 3052382..3052897 (-) 516 WP_035597413.1 cytochrome b/b6 domain-containing protein -
  DCL27_RS14145 (DCL27_14155) cybC 3052903..3053289 (-) 387 WP_035597415.1 cytochrome b562 -
  DCL27_RS14150 (DCL27_14160) deoD 3053503..3054228 (-) 726 WP_005281692.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 489 a.a.        Molecular weight: 55075.91 Da        Isoelectric Point: 4.4068

>NTDB_id=611556 DCL27_RS14110 WP_005281701.1 3048165..3049634(+) (rpoN/rpoN1) [Edwardsiella tarda ATCC 15947 = NBRC 105688 strain ATCC 15947]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQEIQQALESNPLLEQLDPNEEAGDDGVVRENTQEDVVTRDDADPLD
SREALEQREMPDELPLDASWDEIYTAGTPSGTGTDYHDDELPVFQGETTQTLQDYLMWQVELTPFSDTDLAIATAIVDAV
DDTGYLSASLEEILESLGDDRVLPEEIEAVLKRIQRFDPVGVAARDLRDCLLVQLSQFADDTPYLNEARSIVDRHLDLLG
NHDFRNLMRSTKLKEEVLKAAMQLIQSLDPRPGLSINTGESDYVIPDVLVHKHQERWVVELNQDSVPRLRINQHYAALGT
QARNDSDSQFIRSNLQEAKWLIKSLESRNDTLLKVSRCIVEQQQAFFEHGPEAMKPMVLADIAQQVEMHESTISRVTTQK
FLHSPRGIFELKYFFSSHVSTEDGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTLLADQGIMVARRTVAKYRESLSIP
PSNQRKQLV

Nucleotide


Download         Length: 1470 bp        

>NTDB_id=611556 DCL27_RS14110 WP_005281701.1 3048165..3049634(+) (rpoN/rpoN1) [Edwardsiella tarda ATCC 15947 = NBRC 105688 strain ATCC 15947]
ATGAAGCAAGGCTTGCAACTCAGACTCAGCCAGCAGTTGGCCATGACGCCCCAACTGCAACAGGCCATTCGCCTGTTGCA
GCTCTCGACGCTGGAGTTACAGCAAGAGATCCAGCAGGCGTTGGAAAGCAATCCTCTGCTGGAACAACTGGATCCCAATG
AGGAAGCCGGCGACGACGGCGTCGTTCGCGAGAATACACAGGAGGATGTCGTCACACGCGATGATGCCGATCCACTGGAT
AGCCGCGAGGCGTTGGAGCAACGTGAGATGCCGGACGAGCTGCCGCTGGATGCCAGCTGGGACGAGATCTATACCGCTGG
CACCCCCTCAGGCACGGGAACGGATTATCATGACGACGAGTTACCCGTGTTTCAGGGAGAGACGACCCAAACGCTGCAGG
ACTATCTGATGTGGCAGGTCGAACTGACCCCCTTCAGTGATACCGATCTGGCCATCGCTACCGCCATCGTCGATGCCGTC
GACGATACCGGCTATCTCAGCGCCTCGTTGGAGGAGATCCTCGAGAGTCTGGGGGACGATCGCGTCCTACCGGAGGAGAT
CGAGGCCGTCCTCAAGCGCATCCAACGTTTTGATCCGGTCGGTGTCGCCGCGCGTGATCTGCGCGACTGCCTACTGGTCC
AGCTCTCGCAATTCGCCGACGACACCCCCTATCTTAACGAGGCACGTAGCATCGTCGATCGCCACCTCGATCTGTTGGGT
AATCACGATTTCCGAAATCTGATGCGTTCGACTAAGCTAAAAGAAGAGGTGCTCAAGGCGGCGATGCAACTGATCCAGAG
CCTCGACCCGCGGCCGGGACTCTCCATCAATACCGGCGAGTCTGACTATGTCATCCCCGACGTGCTGGTGCATAAACATC
AGGAACGCTGGGTGGTTGAGCTCAATCAGGACAGCGTCCCGCGCCTGCGTATCAACCAACATTACGCCGCGCTGGGCACT
CAGGCGCGTAACGACAGCGACAGCCAGTTCATTCGCAGCAATCTGCAAGAGGCGAAGTGGCTGATCAAGAGTCTAGAGAG
CCGTAACGATACCCTGCTGAAGGTGTCGCGTTGCATCGTGGAGCAGCAGCAAGCGTTCTTCGAGCACGGGCCGGAGGCGA
TGAAGCCGATGGTGCTGGCAGATATCGCTCAACAGGTCGAAATGCACGAGTCGACGATCTCACGCGTGACTACGCAGAAG
TTTCTGCACAGCCCGCGCGGAATCTTTGAACTGAAATATTTCTTCTCCAGCCATGTCAGCACCGAGGACGGCGGCGAGGC
CTCTTCCACGGCCATCCGAGCGCTGGTGAAGAAACTGATCGCGGCAGAAAATCCGGCGAAGCCGCTGAGTGATAGCAAAC
TTACCACTCTCTTGGCGGATCAGGGTATCATGGTCGCCCGGCGTACTGTCGCAAAATACCGAGAGTCTTTGTCCATTCCA
CCGTCCAATCAGCGTAAACAGCTGGTGTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(329-487)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-315)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D4F169

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.367

100

0.481