Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NO473_RS10220 Genome accession   NZ_CP101666
Coordinates   2088488..2088928 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain M171-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2083488..2093928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NO473_RS10200 aroP 2084486..2085859 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  NO473_RS10205 ampE 2085902..2086756 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  NO473_RS10210 ampD 2086753..2087304 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NO473_RS10215 nadC 2087392..2088285 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NO473_RS10220 pilA 2088488..2088928 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  NO473_RS10225 pilB 2088938..2090323 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  NO473_RS10230 hofC 2090313..2091515 (+) 1203 Protein_2006 protein transport protein HofC -
  NO473_RS10235 guaC 2091550..2092593 (-) 1044 WP_001217338.1 GMP reductase -
  NO473_RS10240 - 2092749..2092793 (-) 45 WP_120795372.1 protein YacM -
  NO473_RS10245 coaE 2092818..2093438 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=611548 NO473_RS10220 WP_000360904.1 2088488..2088928(+) (pilA) [Escherichia coli strain M171-1]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=611548 NO473_RS10220 WP_000360904.1 2088488..2088928(+) (pilA) [Escherichia coli strain M171-1]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363