Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   LE167_RS00705 Genome accession   NZ_CP084443
Coordinates   152934..154562 (+) Length   542 a.a.
NCBI ID   WP_070866299.1    Uniprot ID   -
Organism   Staphylococcus lugdunensis strain RMLUG5     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Genomic Context

Location: 147934..159562
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LE167_RS00685 (LE167_00690) ssb 148980..149270 (+) 291 WP_070817190.1 cassette chromosome ssDNA-binding protein -
  LE167_RS00690 (LE167_00695) cch1 149270..151060 (+) 1791 WP_070817191.1 cassette chromosome replicative helicase -
  LE167_RS00695 (LE167_00700) - 151128..151385 (+) 258 WP_070817192.1 hypothetical protein -
  LE167_RS00700 (LE167_00705) ccrA 151563..152912 (+) 1350 WP_070817193.1 cassette chromosome recombinase CcrA Machinery gene
  LE167_RS00705 (LE167_00710) ccrB 152934..154562 (+) 1629 WP_070866299.1 cassette chromosome recombinase CcrB Machinery gene
  LE167_RS00710 (LE167_00715) - 155037..155375 (+) 339 WP_070817195.1 SAUGI family uracil-DNA glycosylase inhibitor -
  LE167_RS00715 (LE167_00720) - 155471..155782 (+) 312 WP_070817196.1 DUF960 family protein -
  LE167_RS00720 (LE167_00725) - 155798..156307 (+) 510 WP_070817197.1 DUF1643 domain-containing protein -
  LE167_RS00725 (LE167_00730) - 156454..156684 (+) 231 WP_225307317.1 hypothetical protein -
  LE167_RS00730 (LE167_00735) - 156983..158194 (-) 1212 WP_070817198.1 hypothetical protein -
  LE167_RS00735 (LE167_00740) - 158325..159323 (+) 999 WP_070817199.1 DNA cytosine methyltransferase -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62719.62 Da        Isoelectric Point: 9.5288

>NTDB_id=611328 LE167_RS00705 WP_070866299.1 152934..154562(+) (ccrB) [Staphylococcus lugdunensis strain RMLUG5]
MQQLKQKRVGIYVRVSTEIQSTEGYSIDGQINQIKEYCQFHHFEVTDIYADRGISGKSMNRPELQRMLKDANEGLIDCVM
VYKTNRLARNTSDLLKIVEDLHKQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
IPLGYEKIPDNKHELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKDNPFSINSITYILVNPFYIGKIQFAK
YKDWNEKRRKGLNDKPIIADGKHPPIISQELWDKVQSRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEKYVMDQILEIVKSDKVLKQVVERVNQDSQIDMAALNHDIAYKQQQFD
EINTKLKNLIQTIEDNPDLTSALKPTIHQYETQLNDITNQINQLKQQQNQEKPSYDTKQIAALLQRIFQNIESMDKSQLK
ALYLTVIDRIDIRKDENHKKQFFVTLKLNNEIIKQLFNNNNLDEVLLSTSSLFLPQTLYFQI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=611328 LE167_RS00705 WP_070866299.1 152934..154562(+) (ccrB) [Staphylococcus lugdunensis strain RMLUG5]
ATGCAACAACTCAAACAAAAACGTGTCGGTATCTATGTTCGTGTATCAACGGAAATCCAAAGTACTGAAGGCTATAGTAT
CGATGGACAAATCAATCAGATTAAAGAATACTGTCAATTTCATCATTTTGAAGTCACAGATATTTACGCTGATCGTGGTA
TCTCAGGAAAATCTATGAACCGCCCTGAGCTCCAACGTATGTTGAAGGATGCGAATGAAGGTCTCATCGATTGTGTCATG
GTATATAAAACAAACCGGCTCGCACGTAATACATCAGACTTACTCAAAATTGTCGAAGATTTACATAAACAAAATGTCGA
ATTCTTCAGCTTATCTGAACGTATGGAAGTCAACACTTCTTCGGGTAAACTGATGTTACAAATACTTGCGAGCTTCTCTG
AATTCGAACGCAATAACATTGTCGAAAATGTATTCATGGGACAAACCAGACGTGCACAAGAAGGCTATTATCAAGGCAAT
ATTCCACTTGGCTATGAGAAAATTCCAGACAATAAACATGAACTCATGATAAACCAACATGAAGCCAATATTGTGAAGTA
TATCTTTGAATCATACGCTAAAGGGCATGGCTATCGTAAAATTGCCAATGCCCTCAATCACAAAGGATATGTTACTAAAA
AAGATAATCCTTTTAGCATCAATTCAATAACCTATATCTTAGTCAATCCATTCTACATTGGTAAAATTCAATTTGCGAAG
TACAAAGATTGGAATGAAAAGCGTCGTAAAGGACTGAATGATAAACCTATTATAGCTGATGGTAAACACCCTCCTATTAT
TAGTCAAGAATTATGGGATAAAGTCCAATCACGCAAAAAACAGGTCAGTCAAAAACCTCAAGTTCATGGAAAAGGAACCA
ATCTATTAACAGGTATTATTCACTGCCCACAATGCGGCGCACCTATGGCGGCAAGTAATACCACTAATACACTCAAAGAT
GGTACCAAGAAGCGAATACGCTATTATTCTTGCAGTAACTTCCGAAACAAAGGCTCAAAAGTATGTTCTGCGAATAGCGT
TAGAGCTGATGTGATTGAGAAATACGTTATGGATCAAATACTCGAAATTGTCAAAAGTGATAAAGTTCTCAAACAAGTTG
TCGAACGTGTTAATCAAGATAGTCAAATTGATATGGCTGCACTTAACCATGATATCGCATATAAGCAACAACAATTTGAT
GAAATAAACACTAAACTAAAAAATCTAATTCAAACCATCGAAGACAATCCAGACTTAACATCTGCACTCAAACCAACCAT
TCATCAATATGAAACACAACTGAATGATATCACAAACCAAATTAATCAACTCAAGCAGCAACAAAACCAAGAAAAACCAT
CTTACGATACGAAACAAATCGCTGCTTTATTACAACGAATATTTCAAAATATAGAATCCATGGATAAATCACAGCTCAAA
GCTTTGTACCTAACGGTTATTGACCGTATTGACATTCGTAAAGATGAGAATCATAAAAAGCAATTCTTTGTCACACTCAA
GCTCAACAATGAGATTATTAAACAACTTTTCAATAATAATAATCTAGACGAAGTGCTCCTCAGCACTTCGTCTTTATTTT
TGCCTCAAACACTCTACTTTCAAATTTAA

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(180-280)

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)

Resolvase, N terminal domain (Resolvase)

(11-156)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

86.531

100

0.865