Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LE166_RS09020 Genome accession   NZ_CP084437
Coordinates   1848801..1850168 (-) Length   455 a.a.
NCBI ID   WP_002459953.1    Uniprot ID   A0A853V434
Organism   Staphylococcus lugdunensis strain RMLUG3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1843801..1855168
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LE166_RS09005 (LE166_09020) cysE 1844914..1845561 (-) 648 WP_172412359.1 serine O-acetyltransferase -
  LE166_RS09010 (LE166_09025) gltX 1845918..1847375 (-) 1458 WP_002459951.1 glutamate--tRNA ligase -
  LE166_RS09015 (LE166_09030) - 1847699..1848766 (-) 1068 WP_002459952.1 PIN/TRAM domain-containing protein -
  LE166_RS09020 (LE166_09035) radA 1848801..1850168 (-) 1368 WP_002459953.1 DNA repair protein RadA Machinery gene
  LE166_RS09025 (LE166_09040) - 1850399..1852858 (-) 2460 WP_002492959.1 ATP-dependent Clp protease ATP-binding subunit -
  LE166_RS09030 (LE166_09045) - 1852872..1853885 (-) 1014 WP_002492974.1 protein arginine kinase -
  LE166_RS09035 (LE166_09050) - 1853878..1854435 (-) 558 WP_002459956.1 UvrB/UvrC motif-containing protein -
  LE166_RS09040 (LE166_09055) - 1854462..1854923 (-) 462 WP_002459957.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 50027.45 Da        Isoelectric Point: 7.0479

>NTDB_id=611285 LE166_RS09020 WP_002459953.1 1848801..1850168(-) (radA) [Staphylococcus lugdunensis strain RMLUG3]
MAKKKIIFECMACGYQSPKWMGKCPNCGAWNQMEEIIEQKAAHPKHGVKAQTATGKVQKLNSIQHESTPRVQTKSQEFNR
VLGGGIVSGSLVLIGGDPGIGKSTLLLQICASLSQQLNVLYITGEESLNQTKLRAERLEEDSSQLNVLAETDLEVIHQTV
SETKPDILVVDSIQTIYHPEIASAPGSVSQVRESTQSLMNIAKQMDIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
EHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVKNPSEMFLEERSTNVPGSTIVSTMEGTRPLLIEVQALVTPTTFNNP
RRMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVRLTEPAVDLSVIVATASSFKDEAVDGLDCYIGEVGLTGEV
RRVSRIEQRVQEAAKLGFKRVILPKTNIGGWTFPDNIQVIGVSTVHEALKYAFKA

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=611285 LE166_RS09020 WP_002459953.1 1848801..1850168(-) (radA) [Staphylococcus lugdunensis strain RMLUG3]
GTGGCCAAGAAGAAAATCATTTTTGAGTGTATGGCATGTGGCTATCAATCTCCTAAATGGATGGGTAAATGTCCTAATTG
TGGTGCTTGGAACCAAATGGAAGAGATTATAGAACAGAAGGCTGCACATCCTAAACATGGGGTTAAAGCACAAACGGCTA
CCGGAAAAGTTCAAAAATTAAACAGTATTCAACATGAATCGACACCACGTGTGCAAACGAAATCTCAAGAGTTTAATCGT
GTGTTAGGTGGGGGGATTGTTAGTGGTTCTTTAGTGTTGATTGGTGGAGATCCAGGTATCGGGAAATCAACACTATTACT
ACAAATCTGTGCCTCGCTTTCGCAACAATTAAACGTGCTGTATATTACAGGTGAGGAATCATTAAATCAAACAAAATTGA
GAGCAGAACGCTTAGAGGAAGACTCTAGTCAATTAAATGTGTTAGCTGAGACAGATTTAGAAGTTATTCACCAAACCGTT
TCAGAAACTAAGCCAGATATTTTAGTGGTGGACTCTATTCAGACGATTTACCATCCTGAGATTGCATCGGCACCCGGATC
TGTTTCTCAAGTGCGTGAGAGTACTCAAAGTTTGATGAATATTGCGAAGCAGATGGATATTGCCACATTTATTGTAGGAC
ATGTGACTAAAGAGGGGCAGATAGCTGGTCCACGATTGTTAGAACACATGGTAGATACAGTGCTTTATTTTGAAGGTGAT
GAACATCATGCGTATCGTATTTTACGTGCTGTTAAAAACCGTTTTGGTTCAACAAATGAAATGGGTATTTTCGAAATGAA
GCAAAGTGGTTTGAAAGGTGTCAAAAACCCGTCCGAAATGTTTTTAGAAGAACGTTCAACAAATGTTCCGGGTTCAACAA
TTGTGTCGACAATGGAAGGAACACGACCATTACTTATTGAAGTACAAGCTTTAGTGACCCCAACAACGTTTAATAATCCA
CGACGTATGGCAACAGGTATTGACCATAACCGTCTGAGCCTGCTTATGGCAGTGCTTGAAAAGAAAGAAAATTATTTATT
GCAGCAACAAGATGCATATATTAAAGTAGCAGGTGGTGTAAGATTAACAGAACCGGCTGTAGACTTAAGTGTAATTGTAG
CAACTGCATCAAGCTTTAAAGATGAAGCGGTTGATGGTTTAGATTGTTATATTGGAGAAGTAGGTTTGACTGGTGAAGTC
AGACGTGTGTCACGTATTGAACAACGCGTTCAAGAAGCAGCTAAACTAGGATTTAAACGTGTGATACTCCCTAAAACAAA
TATCGGCGGATGGACATTTCCTGACAATATACAAGTCATAGGTGTTTCGACAGTACATGAGGCATTGAAATATGCTTTCA
AAGCTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-427)

AAA domain (AAA_25)

(68-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A853V434

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.502

100

0.659
  radA Streptococcus pneumoniae Rx1

59.603

99.56

0.593
  radA Streptococcus pneumoniae D39

59.603

99.56

0.593
  radA Streptococcus pneumoniae R6

59.603

99.56

0.593
  radA Streptococcus pneumoniae TIGR4

59.603

99.56

0.593
  radA Streptococcus mitis NCTC 12261

59.603

99.56

0.593
  radA Streptococcus mitis SK321

59.603

99.56

0.593