Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BASU_RS08855 Genome accession   NC_022081
Coordinates   1776766..1777200 (+) Length   144 a.a.
NCBI ID   WP_015417516.1    Uniprot ID   -
Organism   Bacillus velezensis UCMB5113     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1771766..1782200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BASU_RS08850 (BASU_1663) - 1775266..1776477 (-) 1212 WP_020955852.1 cytochrome P450 -
  BASU_RS08855 (BASU_1664) nucA/comI 1776766..1777200 (+) 435 WP_015417516.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  BASU_RS08860 (BASU_1665) - 1777260..1778015 (+) 756 WP_003154084.1 YoaK family protein -
  BASU_RS08865 (BASU_1666) - 1778049..1778411 (-) 363 WP_007410383.1 hypothetical protein -
  BASU_RS08870 (BASU_1668) - 1778604..1779932 (-) 1329 WP_020955853.1 S8 family peptidase -
  BASU_RS08875 (BASU_1669) - 1780111..1780344 (+) 234 WP_015239897.1 hypothetical protein -
  BASU_RS08880 (BASU_1670) - 1780600..1781307 (+) 708 WP_007410381.1 poly-gamma-glutamate hydrolase family protein -
  BASU_RS08885 (BASU_1671) - 1781367..1781819 (+) 453 WP_007410380.1 OsmC family protein -
  BASU_RS08890 (BASU_1672) - 1781833..1782186 (-) 354 WP_007410379.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15514.44 Da        Isoelectric Point: 7.2418

>NTDB_id=61111 BASU_RS08855 WP_015417516.1 1776766..1777200(+) (nucA/comI) [Bacillus velezensis UCMB5113]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADVRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=61111 BASU_RS08855 WP_015417516.1 1776766..1777200(+) (nucA/comI) [Bacillus velezensis UCMB5113]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGTATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATGTCAGATATGTAACCCCATCAGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.258

86.111

0.493

Multiple sequence alignment