Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LEO81_RS02050 Genome accession   NZ_CP084351
Coordinates   449845..451284 (+) Length   479 a.a.
NCBI ID   WP_017784940.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain 71431     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 444845..456284
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEO81_RS02020 (LEO81_02020) mlaF 445422..446222 (-) 801 WP_005305979.1 phospholipid ABC transporter ATP-binding protein MlaF -
  LEO81_RS02025 (LEO81_02025) - 446430..447425 (+) 996 WP_139397670.1 KpsF/GutQ family sugar-phosphate isomerase -
  LEO81_RS02030 (LEO81_02030) kdsC 447425..447979 (+) 555 WP_201889596.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  LEO81_RS02035 (LEO81_02035) lptC 447976..448536 (+) 561 WP_226014232.1 LPS export ABC transporter periplasmic protein LptC -
  LEO81_RS02040 (LEO81_02040) lptA 448523..449050 (+) 528 WP_099359776.1 lipopolysaccharide transport periplasmic protein LptA -
  LEO81_RS02045 (LEO81_02045) lptB 449054..449779 (+) 726 WP_005305964.1 LPS export ABC transporter ATP-binding protein -
  LEO81_RS02050 (LEO81_02050) rpoN/rpoN1 449845..451284 (+) 1440 WP_017784940.1 RNA polymerase factor sigma-54 Machinery gene
  LEO81_RS02055 (LEO81_02055) hpf 451306..451593 (+) 288 WP_005305957.1 ribosome hibernation promoting factor -
  LEO81_RS02060 (LEO81_02060) ptsN 451596..452042 (+) 447 WP_005305951.1 PTS IIA-like nitrogen regulatory protein PtsN -
  LEO81_RS02065 (LEO81_02065) rapZ 452085..452951 (+) 867 WP_005305946.1 RNase adapter RapZ -
  LEO81_RS02070 (LEO81_02070) npr 452972..453244 (+) 273 WP_005305943.1 PTS phosphocarrier protein NPr -
  LEO81_RS02075 (LEO81_02075) - 453260..455164 (-) 1905 WP_201889597.1 transcription antiterminator -
  LEO81_RS02080 (LEO81_02080) - 455269..456009 (-) 741 WP_201889598.1 KDGP aldolase family protein -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53876.83 Da        Isoelectric Point: 4.3584

>NTDB_id=610743 LEO81_RS02050 WP_017784940.1 449845..451284(+) (rpoN/rpoN1) [Aeromonas dhakensis strain 71431]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENDVQETSPVDQVDVSQLDSSDAMAQDQMP
DELPVDTTWEEVYSAGTAQSAGPIHDGEDSIYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVSLED
IQAAVSSEDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLNEAREVILEHMDLLGNRDYRTLARK
TKLRENDLKEVLDLIQQLEPRPGNGVIQSESQYVIPDVSVVKKGNKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=610743 LEO81_RS02050 WP_017784940.1 449845..451284(+) (rpoN/rpoN1) [Aeromonas dhakensis strain 71431]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGACAACAACCCCTTGCTGGAGCAAGAGGAAAACGACG
TTCAGGAGACCAGCCCGGTCGACCAGGTGGACGTCAGCCAGCTCGATTCCAGCGATGCCATGGCCCAGGACCAGATGCCG
GACGAACTGCCGGTGGATACCACCTGGGAAGAGGTCTACTCGGCCGGCACGGCCCAGAGCGCAGGCCCCATCCATGACGG
GGAAGACAGCATCTATCAGGGTGAGACCACCGAGAACCTGCAGGACTACCTGTTGTGGCAGATGCGCTTGACCCCGTTCA
GCGACGTGGATGCCGCCATCGCCCTCGCCATCATAGATGCTATCGACGAATCCGGTTATCTGACGGTGTCGCTTGAGGAT
ATTCAGGCGGCGGTGAGCAGCGAGGATGTCGAGGTGGAACTCGATGAAGTGGAGGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCCCGCTCGGTGCAGGAGTGCCTGCTGATCCAGCTGGGCCAGTACTCCCCTGACTTGCCCT
GGCTCAACGAAGCCAGGGAGGTGATCCTCGAGCACATGGATCTGCTCGGCAACCGCGATTACCGCACCCTGGCCCGCAAG
ACCAAGCTGAGAGAGAACGACCTCAAGGAGGTGCTGGATCTCATCCAGCAGCTGGAGCCGCGGCCCGGCAACGGCGTCAT
CCAGAGCGAATCCCAGTACGTGATCCCGGATGTTTCCGTGGTCAAGAAGGGCAACAAGTGGGTGGTCGAGCTCAACCCTG
ACTCGGCCCCGCGCATCCGGGTCAACGAAACCTATGCCGCCATGGCCAGACAGGCCCGCACCAGTCAGGATACCCAATTC
ATCCGCTCCCACCTGCAGGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGCCGCAACGACACCCTGCTCAAGGTGGCCAG
CTGCATCGTCGAGCAGCAGCAGGGCTTCTTCGAATACGGTGAAGAGGCCATGAAACCCATGGTGCTCAACGACATCGCCG
AAGCGGTGGAGATGCACGAGTCGACCATCTCGCGGGTGACCACCCAGAAGTACATGCACACGCCGCGCGGCATCTTCGAA
CTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAGGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCGGAGAATCCCGCCAAACCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.866

100

0.486