Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LEO79_RS12665 Genome accession   NZ_CP084350
Coordinates   2723524..2724963 (+) Length   479 a.a.
NCBI ID   WP_226043682.1    Uniprot ID   -
Organism   Aeromonas caviae strain 71442     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2718524..2729963
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEO79_RS12635 (LEO79_12635) mlaF 2719102..2719902 (-) 801 WP_029314846.1 phospholipid ABC transporter ATP-binding protein MlaF -
  LEO79_RS12640 (LEO79_12640) - 2720109..2721104 (+) 996 WP_042880205.1 KpsF/GutQ family sugar-phosphate isomerase -
  LEO79_RS12645 (LEO79_12645) kdsC 2721104..2721658 (+) 555 WP_226043681.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  LEO79_RS12650 (LEO79_12650) lptC 2721655..2722215 (+) 561 WP_039040473.1 LPS export ABC transporter periplasmic protein LptC -
  LEO79_RS12655 (LEO79_12655) lptA 2722202..2722720 (+) 519 WP_010675873.1 lipopolysaccharide transport periplasmic protein LptA -
  LEO79_RS12660 (LEO79_12660) lptB 2722733..2723458 (+) 726 WP_010675872.1 LPS export ABC transporter ATP-binding protein -
  LEO79_RS12665 (LEO79_12665) rpoN/rpoN1 2723524..2724963 (+) 1440 WP_226043682.1 RNA polymerase factor sigma-54 Machinery gene
  LEO79_RS12670 (LEO79_12670) hpf 2724984..2725271 (+) 288 WP_005305957.1 ribosome hibernation promoting factor -
  LEO79_RS12675 (LEO79_12675) ptsN 2725274..2725720 (+) 447 WP_010675870.1 PTS IIA-like nitrogen regulatory protein PtsN -
  LEO79_RS12680 (LEO79_12680) rapZ 2725761..2726627 (+) 867 WP_010675869.1 RNase adapter RapZ -
  LEO79_RS12685 (LEO79_12685) npr 2726648..2726920 (+) 273 WP_010675868.1 PTS phosphocarrier protein NPr -
  LEO79_RS12690 (LEO79_12690) - 2726936..2728840 (-) 1905 WP_226043683.1 transcription antiterminator -
  LEO79_RS12695 (LEO79_12695) - 2728944..2729684 (-) 741 WP_226043684.1 2-dehydro-3-deoxy-phosphogluconate aldolase -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53762.51 Da        Isoelectric Point: 4.3305

>NTDB_id=610725 LEO79_RS12665 WP_226043682.1 2723524..2724963(+) (rpoN/rpoN1) [Aeromonas caviae strain 71442]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEAQETSQTETQDASQLDSSDAMAQESMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTATLED
IQAAVSSEEVEVELDEIEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLDEAREVIREHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNGIIQSESQYVIPDVSVVKKGSEWVVELNPDSAPRIRVNEAYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=610725 LEO79_RS12665 WP_226043682.1 2723524..2724963(+) (rpoN/rpoN1) [Aeromonas caviae strain 71442]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCACAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGACAACAACCCCTTGCTGGAGCAAGAGGAGAACGAGG
CGCAGGAAACCAGTCAGACAGAGACACAGGACGCCAGTCAACTCGACTCCAGCGATGCCATGGCCCAGGAAAGCATGCCG
GATGAACTGCCGGTGGACACCACCTGGGACGAGGTCTACTCGGCCGGTACGGCCCAGAGTGCAGGCCCCATCCATGACGG
GGAAGACAGCGTCTATCAGGGGGAGACCACCGAGAACCTGCAGGACTACCTGCTGTGGCAGATGCGGCTCACCCCGTTCA
GTGATGTGGATGCTGCCATCGCCCTTGCCATCATCGATGCCATCGACGAATCCGGGTATCTGACTGCCACCCTCGAGGAT
ATTCAGGCTGCAGTCAGCAGCGAGGAGGTAGAAGTCGAACTGGATGAGATCGAAGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCCCGTTCGGTGCAAGAGTGTCTGCTGATCCAGCTCGGCCAGTATTCGCCAGACCTGCCCT
GGCTCGACGAAGCCAGAGAGGTGATCCGCGAACACATGGACCTGCTTGGCAATCGCGACTACCGCACCCTGGCTCGCAAG
ACCCGCCTCAAGGAGAGCGATCTCAAGGAGGTCCTGGATCTCATCCAGCAACTGGAGCCCAGACCCGGCAACGGCATCAT
CCAGAGTGAATCCCAGTACGTGATCCCGGATGTGTCCGTGGTCAAGAAGGGCAGCGAGTGGGTGGTTGAACTCAATCCGG
ATTCTGCTCCGCGCATCCGTGTCAACGAGGCCTATGCGGCCATGGCGCGCAATGCCCGCAGCAGCACGGATACCCAGTTC
ATCCGTTCTCATTTGCAGGAGGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGTAACGACACCCTGCTCAAGGTGGCCAG
CTGCATCGTCGAGCAGCAGCAGGGCTTTTTCGAGTACGGCGAAGAGGCCATGAAACCCATGGTACTCAACGACATCGCGG
AAGCGGTGGAGATGCATGAGTCGACCATTTCACGGGTCACGACCCAGAAGTACATGCATACGCCCCGCGGCATTTTTGAA
CTGAAGTTTTTTTTCTCCAGTCACGTCAATACGGAGGGAGGAGGAGAGTGCTCATCGACCGCTATTCGCGCCCTGATCAA
GAAGCTGGTGACGGCGGAAAACCCCGCCAAGCCGCTTAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.472

100

0.482