Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LEO73_RS02045 Genome accession   NZ_CP084349
Coordinates   445259..446698 (+) Length   479 a.a.
NCBI ID   WP_017784940.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain 71453     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 440259..451698
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEO73_RS02015 (LEO73_02015) mlaF 440836..441636 (-) 801 WP_005305979.1 phospholipid ABC transporter ATP-binding protein MlaF -
  LEO73_RS02020 (LEO73_02020) - 441844..442839 (+) 996 WP_005305976.1 KpsF/GutQ family sugar-phosphate isomerase -
  LEO73_RS02025 (LEO73_02025) kdsC 442839..443393 (+) 555 WP_043171640.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  LEO73_RS02030 (LEO73_02030) lptC 443390..443950 (+) 561 WP_043171639.1 LPS export ABC transporter periplasmic protein LptC -
  LEO73_RS02035 (LEO73_02035) lptA 443937..444464 (+) 528 WP_010635928.1 lipopolysaccharide transport periplasmic protein LptA -
  LEO73_RS02040 (LEO73_02040) lptB 444468..445193 (+) 726 WP_005305964.1 LPS export ABC transporter ATP-binding protein -
  LEO73_RS02045 (LEO73_02045) rpoN/rpoN1 445259..446698 (+) 1440 WP_017784940.1 RNA polymerase factor sigma-54 Machinery gene
  LEO73_RS02050 (LEO73_02050) hpf 446720..447007 (+) 288 WP_005305957.1 ribosome hibernation promoting factor -
  LEO73_RS02055 (LEO73_02055) ptsN 447010..447456 (+) 447 WP_005305951.1 PTS IIA-like nitrogen regulatory protein PtsN -
  LEO73_RS02060 (LEO73_02060) rapZ 447499..448365 (+) 867 WP_005305946.1 RNase adapter RapZ -
  LEO73_RS02065 (LEO73_02065) npr 448386..448658 (+) 273 WP_005305943.1 PTS phosphocarrier protein NPr -
  LEO73_RS02070 (LEO73_02070) - 448674..450578 (-) 1905 WP_201889597.1 transcription antiterminator -
  LEO73_RS02075 (LEO73_02075) - 450683..451423 (-) 741 WP_201889598.1 2-dehydro-3-deoxy-phosphogluconate aldolase -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53876.83 Da        Isoelectric Point: 4.3584

>NTDB_id=610687 LEO73_RS02045 WP_017784940.1 445259..446698(+) (rpoN/rpoN1) [Aeromonas dhakensis strain 71453]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENDVQETSPVDQVDVSQLDSSDAMAQDQMP
DELPVDTTWEEVYSAGTAQSAGPIHDGEDSIYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVSLED
IQAAVSSEDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLNEAREVILEHMDLLGNRDYRTLARK
TKLRENDLKEVLDLIQQLEPRPGNGVIQSESQYVIPDVSVVKKGNKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=610687 LEO73_RS02045 WP_017784940.1 445259..446698(+) (rpoN/rpoN1) [Aeromonas dhakensis strain 71453]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGACAACAACCCCTTGCTGGAGCAAGAGGAAAACGACG
TTCAGGAGACCAGCCCGGTCGACCAGGTGGACGTCAGCCAGCTCGATTCCAGCGATGCCATGGCCCAGGACCAGATGCCG
GACGAACTGCCGGTGGATACCACCTGGGAAGAGGTCTACTCGGCCGGCACGGCCCAGAGCGCAGGCCCCATCCATGACGG
GGAAGACAGCATCTATCAGGGTGAGACCACCGAGAACCTGCAGGACTACCTGTTGTGGCAGATGCGCTTGACCCCGTTCA
GCGACGTGGATGCCGCCATCGCCCTCGCCATCATAGATGCTATCGACGAATCCGGTTATCTGACGGTGTCGCTTGAGGAT
ATTCAGGCGGCGGTGAGCAGCGAGGATGTCGAGGTGGAACTCGATGAAGTGGAGGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCCCGCTCGGTGCAGGAGTGCCTGCTGATCCAGCTGGGCCAGTACTCCCCTGACTTGCCCT
GGCTCAACGAAGCCAGGGAGGTGATCCTCGAGCACATGGATCTGCTCGGCAACCGCGATTACCGCACCCTGGCCCGCAAG
ACCAAGCTGAGAGAGAACGACCTCAAGGAGGTGCTGGATCTCATCCAGCAGCTGGAGCCGCGGCCCGGCAACGGCGTCAT
CCAGAGCGAATCCCAGTACGTGATCCCGGATGTTTCCGTGGTCAAGAAGGGCAACAAGTGGGTGGTCGAGCTCAACCCTG
ACTCGGCCCCGCGCATCCGGGTCAACGAAACCTATGCCGCCATGGCCAGACAGGCCCGCACCAGTCAGGATACCCAATTC
ATCCGCTCCCACCTGCAGGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGCCGCAACGACACCCTGCTCAAGGTGGCCAG
CTGCATCGTCGAGCAGCAGCAGGGCTTCTTCGAATACGGTGAAGAGGCCATGAAACCCATGGTGCTCAACGACATCGCCG
AAGCGGTGGAGATGCACGAGTCGACCATCTCGCGGGTGACCACCCAGAAGTACATGCACACGCCGCGCGGCATCTTCGAA
CTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAGGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCGGAGAATCCCGCCAAACCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.866

100

0.486