Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LDO52_RS11975 Genome accession   NZ_CP084300
Coordinates   2477307..2478107 (-) Length   266 a.a.
NCBI ID   WP_005172689.1    Uniprot ID   -
Organism   Acinetobacter pseudolwoffii strain XMC5X702     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2468505..2484737 2477307..2478107 within 0


Gene organization within MGE regions


Location: 2468505..2484737
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDO52_RS11930 (LDO52_11930) - 2468505..2469104 (-) 600 WP_004280831.1 IS607 family transposase -
  LDO52_RS11935 (LDO52_11935) - 2469104..2469745 (-) 642 WP_225553143.1 hypothetical protein -
  LDO52_RS11940 (LDO52_11940) - 2469835..2470482 (-) 648 WP_159138483.1 4'-phosphopantetheinyl transferase family protein -
  LDO52_RS11945 (LDO52_11945) der 2470629..2472038 (-) 1410 WP_005095683.1 ribosome biogenesis GTPase Der -
  LDO52_RS11950 (LDO52_11950) bamB 2472210..2473358 (-) 1149 WP_005095682.1 outer membrane protein assembly factor BamB -
  LDO52_RS11955 (LDO52_11955) - 2473355..2474050 (-) 696 WP_005095680.1 YfgM family protein -
  LDO52_RS11960 (LDO52_11960) hisS 2474081..2475376 (-) 1296 WP_005095678.1 histidine--tRNA ligase -
  LDO52_RS11965 (LDO52_11965) ispG 2475380..2476501 (-) 1122 WP_225553145.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  LDO52_RS11970 (LDO52_11970) - 2476525..2477316 (-) 792 WP_225553146.1 helix-turn-helix domain-containing protein -
  LDO52_RS11975 (LDO52_11975) pilF 2477307..2478107 (-) 801 WP_005172689.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  LDO52_RS11980 (LDO52_11980) rlmN 2478122..2479357 (-) 1236 WP_225553148.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  LDO52_RS11985 (LDO52_11985) ndk 2479480..2479911 (-) 432 WP_004647460.1 nucleoside-diphosphate kinase -
  LDO52_RS11990 (LDO52_11990) iscX 2480022..2480222 (-) 201 WP_005172695.1 Fe-S cluster assembly protein IscX -
  LDO52_RS11995 (LDO52_11995) - 2480310..2480861 (-) 552 WP_225554256.1 phosphatase PAP2 family protein -
  LDO52_RS12000 (LDO52_12000) - 2480892..2482163 (-) 1272 WP_005095661.1 glycosyltransferase family 4 protein -
  LDO52_RS12005 (LDO52_12005) - 2482431..2483182 (+) 752 WP_373685564.1 IS5 family transposase -
  LDO52_RS12010 (LDO52_12010) ilvC 2483721..2484737 (-) 1017 WP_005172704.1 ketol-acid reductoisomerase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30021.09 Da        Isoelectric Point: 9.0728

>NTDB_id=610525 LDO52_RS11975 WP_005172689.1 2477307..2478107(-) (pilF) [Acinetobacter pseudolwoffii strain XMC5X702]
MRNLTSKIALISTVCVSLALVACQTPDLGQKDPEKATKVRTQLAAEYLRTGDLDAAKRALDQALETTPRDSQANMMMGVL
LQQEGSQLNLEKADAYFKRAISADPKNAQARNNYGTYLYQLERYNDAIEQFNIAGATLGYDQRFRALENMGRIYLKMGDV
VNAEKSFKQALQANRDSYISMLELSEIFYLNQQFPAATQLYQQFARGVGQKNQGARALWVGIRTARASGDQLGMQVLVNQ
LRALFPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=610525 LDO52_RS11975 WP_005172689.1 2477307..2478107(-) (pilF) [Acinetobacter pseudolwoffii strain XMC5X702]
TTGAGAAATCTAACATCTAAAATTGCTTTGATTTCAACAGTATGTGTGTCTTTAGCTTTGGTTGCATGTCAAACGCCAGA
TTTAGGTCAGAAAGATCCTGAAAAAGCCACCAAAGTCCGTACCCAATTGGCGGCAGAATATTTAAGAACAGGTGACCTAG
ATGCAGCCAAACGTGCGCTGGATCAGGCACTCGAAACGACTCCGCGTGATTCGCAAGCGAATATGATGATGGGCGTGCTA
TTGCAACAGGAAGGTAGTCAGCTCAATCTTGAGAAAGCAGATGCTTATTTCAAGCGTGCAATTTCGGCAGATCCGAAAAA
TGCACAGGCACGCAACAACTATGGCACGTATTTATATCAGCTAGAACGCTATAATGATGCGATCGAGCAGTTTAACATTG
CCGGTGCAACTTTAGGTTATGATCAGCGTTTCCGTGCACTGGAAAATATGGGACGTATTTACCTGAAAATGGGCGATGTG
GTGAATGCTGAAAAATCATTCAAACAGGCGCTTCAGGCGAATCGCGATTCATATATATCAATGTTAGAGTTGTCAGAAAT
TTTTTATTTAAACCAGCAGTTCCCGGCAGCGACACAACTGTATCAACAGTTTGCGCGTGGTGTGGGACAGAAAAATCAGG
GTGCCCGTGCGCTCTGGGTTGGCATTCGCACTGCGCGTGCCAGTGGTGATCAGTTGGGGATGCAGGTATTGGTGAACCAG
TTACGTGCATTGTTTCCTGAAAGCCAGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(97-129)

Tetratricopeptide repeat (TPR_16)

(150-204)

Tetratricopeptide repeat (TPR_14)

(40-79)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

76.538

97.744

0.748
  pilF Acinetobacter baylyi ADP1

69.804

95.865

0.669