Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NMK62_RS02875 Genome accession   NZ_CP101310
Coordinates   574696..576129 (-) Length   477 a.a.
NCBI ID   WP_000809065.1    Uniprot ID   -
Organism   Escherichia coli strain 99-3311     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 569696..581129
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMK62_RS02840 (NMK62_02840) elbB 570388..571041 (+) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -
  NMK62_RS02845 (NMK62_02845) mtgA 571038..571766 (+) 729 WP_000047097.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  NMK62_RS02850 (NMK62_02850) yrbL 571763..572395 (-) 633 WP_000620410.1 PhoP regulatory network protein YrbL -
  NMK62_RS02855 (NMK62_02855) npr 572608..572880 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  NMK62_RS02860 (NMK62_02860) rapZ 572877..573731 (-) 855 WP_000243741.1 RNase adapter RapZ -
  NMK62_RS02865 (NMK62_02865) ptsN 573777..574268 (-) 492 WP_001400765.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NMK62_RS02870 (NMK62_02870) hpf 574386..574673 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  NMK62_RS02875 (NMK62_02875) rpoN/rpoN1 574696..576129 (-) 1434 WP_000809065.1 RNA polymerase factor sigma-54 Machinery gene
  NMK62_RS02880 (NMK62_02880) lptB 576177..576902 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  NMK62_RS02885 (NMK62_02885) lptA 576909..577466 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  NMK62_RS02890 (NMK62_02890) lptC 577435..578010 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  NMK62_RS02895 (NMK62_02895) kdsC 578007..578573 (-) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  NMK62_RS02900 (NMK62_02900) kdsD 578594..579580 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  NMK62_RS02905 (NMK62_02905) yrbG 579594..580571 (-) 978 WP_000922872.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54023.82 Da        Isoelectric Point: 4.3639

>NTDB_id=610331 NMK62_RS02875 WP_000809065.1 574696..576129(-) (rpoN/rpoN1) [Escherichia coli strain 99-3311]
MKQGLQLRLSQQLTMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=610331 NMK62_RS02875 WP_000809065.1 574696..576129(-) (rpoN/rpoN1) [Escherichia coli strain 99-3311]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGACGATGACGCCACAGCTTCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACGCCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAATGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAAGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCACTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAGCCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.024

100

0.463