Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   NMK62_RS02500 Genome accession   NZ_CP101310
Coordinates   496716..497840 (+) Length   374 a.a.
NCBI ID   WP_000228550.1    Uniprot ID   -
Organism   Escherichia coli strain 99-3311     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 491716..502840
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMK62_RS02475 (NMK62_02475) mscL 491842..492252 (-) 411 WP_000022441.1 large-conductance mechanosensitive channel protein MscL -
  NMK62_RS02480 (NMK62_02480) trkA 492382..493758 (-) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  NMK62_RS02485 (NMK62_02485) rsmB 493780..495069 (-) 1290 WP_000744779.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  NMK62_RS02490 (NMK62_02490) fmt 495115..496062 (-) 948 WP_000004448.1 methionyl-tRNA formyltransferase -
  NMK62_RS02495 (NMK62_02495) def 496077..496586 (-) 510 WP_000114984.1 peptide deformylase -
  NMK62_RS02500 (NMK62_02500) dprA 496716..497840 (+) 1125 WP_000228550.1 DNA-protecting protein DprA Machinery gene
  NMK62_RS02505 (NMK62_02505) smg 497812..498285 (+) 474 WP_000460670.1 DUF494 family protein Smg -
  NMK62_RS02510 (NMK62_02510) yrdD 498314..498856 (+) 543 WP_001129723.1 type I DNA topoisomerase -
  NMK62_RS02515 (NMK62_02515) tsaC 498861..499433 (+) 573 WP_001301437.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  NMK62_RS02520 (NMK62_02520) aroE 499438..500256 (+) 819 WP_000451242.1 shikimate dehydrogenase -
  NMK62_RS02525 (NMK62_02525) yrdB 500253..500510 (+) 258 WP_001070559.1 DUF1488 domain-containing protein -
  NMK62_RS02530 (NMK62_02530) yrdA 500486..501040 (-) 555 WP_022581407.1 gamma carbonic anhydrase family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 41002.96 Da        Isoelectric Point: 6.3366

>NTDB_id=610330 NMK62_RS02500 WP_000228550.1 496716..497840(+) (dprA) [Escherichia coli strain 99-3311]
MVDTEIWLRLMSISSLYGDDMVRIAHWVAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLAKHGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=610330 NMK62_RS02500 WP_000228550.1 496716..497840(+) (dprA) [Escherichia coli strain 99-3311]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTACGGCGATGATATGGTCCGTATCGCTCACTG
GGTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCACGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGAAGCATGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTAGCGCATAAAGCAGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCGCGTCGCCATGCCCGACTGGCTACCAGTTTGCT
TGAACATGGTGGGGCACTTGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterProScan.

DprA winged helix domain (WHD_DprA)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.78

98.663

0.481
  dprA Glaesserella parasuis strain SC1401

45.401

90.107

0.409
  dprA Vibrio cholerae O1 biovar El Tor strain E7946

49.032

82.888

0.406
  dprA Vibrio cholerae strain A1552

49.032

82.888

0.406
  dprA Haemophilus influenzae Rd KW20

43.917

90.107

0.396
  dprA Legionella pneumophila strain ERS1305867

44.545

88.235

0.393