Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M493_RS00525 Genome accession   NC_022080
Coordinates   99907..101280 (+) Length   457 a.a.
NCBI ID   WP_020958335.1    Uniprot ID   S5Z0Q7
Organism   Geobacillus genomosp. 3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 94907..106280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M493_RS00505 (M493_00725) ctsR 95278..95739 (+) 462 WP_020958331.1 transcriptional regulator CtsR -
  M493_RS00510 (M493_00730) - 95754..96302 (+) 549 WP_020958332.1 UvrB/UvrC motif-containing protein -
  M493_RS00515 (M493_00735) - 96307..97398 (+) 1092 WP_020958333.1 protein arginine kinase -
  M493_RS00520 (M493_00740) clpC 97395..99830 (+) 2436 WP_020958334.1 ATP-dependent protease ATP-binding subunit ClpC -
  M493_RS00525 (M493_00745) radA 99907..101280 (+) 1374 WP_020958335.1 DNA repair protein RadA Machinery gene
  M493_RS00530 (M493_00750) - 101485..102579 (+) 1095 WP_020958336.1 PIN/TRAM domain-containing protein -
  M493_RS00535 (M493_00755) ispD 102601..103287 (+) 687 WP_020958337.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  M493_RS00540 (M493_00760) ispF 103302..103784 (+) 483 WP_020958338.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  M493_RS00545 (M493_00765) gltX 103979..105448 (+) 1470 WP_020958339.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49607.26 Da        Isoelectric Point: 7.9955

>NTDB_id=61033 M493_RS00525 WP_020958335.1 99907..101280(+) (radA) [Geobacillus genomosp. 3]
MVKKKTKFVCQECGYESAKWLGRCPGCQTWNSFVEEIERAKPAVRGTFLHSEPSETAKPIPITAVQAVQEPRIETKSAEL
NRVLGGGIVTGSLVLIGGDPGIGKSTLLLQTSAQLAAAGHTVLYISGEESTKQVKLRAGRLRAESDQLYVLAEANLEYIV
TAIETIRPACVVIDSIQTMYRTDITSAPGSVAQVRECTAELMKIAKTKGVAIFIVGHVTKEGAIAGPRILEHMVDTVLYF
EGERHHTYRILRAVKNRFGSTNEIGIFEMRDMGLREVENPSEVFLEERSRGAAGSTVVAAMEGTRPVLVEIQALVSPTSF
GNPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDRPTNPADVIIGEVGLT
GEVRRVSRIEQRVQEAVKLGFSRVIVPKNNLAGWQPPVGVNVIGVSHVTEALEHTML

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=61033 M493_RS00525 WP_020958335.1 99907..101280(+) (radA) [Geobacillus genomosp. 3]
ATGGTTAAAAAGAAAACGAAATTTGTTTGCCAGGAATGTGGGTATGAATCCGCCAAATGGCTCGGCCGCTGTCCAGGCTG
TCAAACGTGGAACTCGTTCGTTGAAGAAATCGAGCGGGCGAAGCCGGCTGTGCGCGGGACGTTTCTTCATTCGGAGCCGT
CGGAAACGGCCAAACCGATTCCGATTACGGCCGTTCAGGCGGTGCAAGAGCCGCGTATTGAAACGAAAAGTGCGGAGCTC
AATCGCGTGCTCGGTGGAGGGATTGTGACAGGTTCGCTCGTCTTAATTGGCGGCGACCCGGGCATCGGCAAGTCGACGTT
GCTGTTGCAGACATCGGCGCAGCTCGCTGCAGCCGGACACACCGTGTTGTATATATCCGGCGAGGAATCGACGAAGCAAG
TGAAGCTGCGCGCCGGACGTCTTCGCGCTGAATCTGATCAACTGTATGTGTTAGCGGAGGCGAATTTGGAATATATTGTA
ACAGCAATTGAAACGATTCGGCCTGCCTGTGTCGTCATTGATTCGATTCAGACGATGTACCGGACGGATATTACGTCAGC
GCCGGGCAGCGTCGCCCAAGTGCGCGAGTGTACTGCCGAGTTGATGAAAATCGCCAAGACAAAAGGCGTCGCCATTTTTA
TCGTCGGCCATGTGACGAAAGAAGGGGCGATCGCCGGGCCGCGCATCTTAGAGCATATGGTTGACACTGTTCTTTATTTT
GAAGGCGAGCGGCATCACACGTACCGTATTTTGCGGGCGGTGAAAAACCGCTTTGGCTCGACGAATGAAATCGGTATTTT
TGAAATGCGCGACATGGGGTTAAGAGAAGTGGAAAACCCGTCTGAAGTGTTTTTGGAAGAGCGGTCGCGCGGAGCGGCCG
GATCGACGGTAGTGGCGGCCATGGAAGGGACGCGTCCGGTATTGGTGGAAATCCAGGCGCTCGTCTCGCCGACAAGCTTC
GGCAACCCGAGGCGGATGGCGACCGGCCTTGATCATAACCGTGTGTCGCTTCTGATGGCTGTGCTCGAAAAGCGGGTCGG
ACTGCTGTTGCAAAATCAAGATGCTTATTTGAAAGTGGCAGGAGGAGTGAAGCTTGACGAACCGGCCATCGATTTGGCGG
TCGCCGTCAGCATTGCCTCGAGTTTCCGCGACCGGCCGACAAATCCGGCCGATGTCATTATCGGCGAAGTCGGCTTGACC
GGGGAGGTACGCCGCGTTTCCCGTATTGAACAGCGCGTACAGGAGGCTGTCAAGTTAGGTTTTTCCCGCGTCATTGTGCC
AAAAAACAATTTGGCTGGCTGGCAGCCGCCTGTGGGCGTTAACGTGATCGGTGTTTCGCATGTGACCGAGGCGCTTGAGC
ATACGATGTTATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(66-222)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S5Z0Q7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

72.867

100

0.729
  radA Streptococcus mitis NCTC 12261

63.537

100

0.637
  radA Streptococcus mitis SK321

63.319

100

0.635
  radA Streptococcus pneumoniae Rx1

63.1

100

0.632
  radA Streptococcus pneumoniae D39

63.1

100

0.632
  radA Streptococcus pneumoniae R6

63.1

100

0.632
  radA Streptococcus pneumoniae TIGR4

63.1

100

0.632

Multiple sequence alignment