Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NMK57_RS22125 Genome accession   NZ_CP101305
Coordinates   4395728..4396168 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain 2002-3211     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4390728..4401168
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMK57_RS22105 (NMK57_22105) aroP 4391726..4393099 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  NMK57_RS22110 (NMK57_22110) ampE 4393142..4393996 (-) 855 WP_000172006.1 beta-lactamase regulator AmpE -
  NMK57_RS22115 (NMK57_22115) ampD 4393993..4394544 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NMK57_RS22120 (NMK57_22120) nadC 4394632..4395525 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NMK57_RS22125 (NMK57_22125) pilA 4395728..4396168 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  NMK57_RS22130 (NMK57_22130) pilB 4396178..4397563 (+) 1386 WP_001025149.1 type II secretion system protein GspE Machinery gene
  NMK57_RS22135 (NMK57_22135) hofC 4397553..4398755 (+) 1203 WP_000157250.1 protein transport protein HofC -
  NMK57_RS22140 (NMK57_22140) guaC 4398790..4399833 (-) 1044 WP_001217338.1 GMP reductase -
  NMK57_RS22145 (NMK57_22145) - 4399989..4400033 (-) 45 WP_120795372.1 protein YacM -
  NMK57_RS22150 (NMK57_22150) coaE 4400058..4400678 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=610300 NMK57_RS22125 WP_000360904.1 4395728..4396168(+) (pilA) [Escherichia coli strain 2002-3211]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=610300 NMK57_RS22125 WP_000360904.1 4395728..4396168(+) (pilA) [Escherichia coli strain 2002-3211]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCTGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363