Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   K9N64_RS05435 Genome accession   NZ_CP084248
Coordinates   652032..653402 (+) Length   456 a.a.
NCBI ID   WP_002293096.1    Uniprot ID   A0A4Y3JR63
Organism   Enterococcus lactis strain DH9003     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 647032..658402
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K9N64_RS05410 (K9N64_05400) - 648299..648625 (+) 327 WP_002310464.1 hypothetical protein -
  K9N64_RS05415 (K9N64_05405) - 648742..649086 (-) 345 WP_002310463.1 hypothetical protein -
  K9N64_RS05420 (K9N64_05410) - 649162..650409 (-) 1248 WP_002310461.1 glutamate-5-semialdehyde dehydrogenase -
  K9N64_RS05425 (K9N64_05415) proB 650406..651212 (-) 807 WP_002344833.1 glutamate 5-kinase -
  K9N64_RS05430 (K9N64_05420) - 651442..651969 (+) 528 WP_002344834.1 dUTP diphosphatase -
  K9N64_RS05435 (K9N64_05425) radA 652032..653402 (+) 1371 WP_002293096.1 DNA repair protein RadA Machinery gene
  K9N64_RS05440 (K9N64_05430) - 653436..654593 (+) 1158 WP_002287448.1 PIN/TRAM domain-containing protein -
  K9N64_RS05445 (K9N64_05435) gltX 654763..656223 (+) 1461 WP_002293095.1 glutamate--tRNA ligase -
  K9N64_RS05450 (K9N64_05440) epsC 656543..657079 (+) 537 WP_002293094.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49935.60 Da        Isoelectric Point: 7.7416

>NTDB_id=610284 K9N64_RS05435 WP_002293096.1 652032..653402(+) (radA) [Enterococcus lactis strain DH9003]
MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTDRRTRTSLTGEKAKPTKIADVVPKKEPRVKTKLEELN
RVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEVANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECFIGEIGLTG
EIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAETLKRVFR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=610284 K9N64_RS05435 WP_002293096.1 652032..653402(+) (radA) [Enterococcus lactis strain DH9003]
ATGGCAAAAAAATCAAAAGTACAATTTGTTTGCCAAAATTGCGGGTATGTGTCGCCTAAGTTCTTGGGACGCTGCCCAAA
TTGCGGAAAATGGAATACGATGGTCGAAGAAATCGAACAAGATACAACAGACCGCAGAACGCGGACAAGTTTGACAGGAG
AAAAAGCGAAACCAACAAAAATCGCAGATGTCGTCCCTAAAAAAGAACCTCGGGTCAAAACGAAATTAGAAGAACTGAAC
CGTGTGCTAGGTGGAGGCGTGGTTCCGGGATCCATGGTCTTGATTGGTGGAGATCCAGGTATTGGTAAATCCACTTTGCT
GTTACAAGTTTCTCAACAATTAGCAGCCATAGGCGGGAAAGTCTTGTATGTATCCGGCGAAGAAAGCGCAGAACAGATAA
AATTACGCGCGGAGCGCCTCGGAAGTATCAACACAGAATTTTATCTATACGCTGAAACAGATATGAATGAAATCAGTCGT
GCCATCGAACATATCTCCCCTGATTATGTTATTATTGATTCCATCCAAACGATGACGCAGCCAGACATCACAAGTGTGGC
AGGAAGCGTCAGCCAAGTAAGAGAAACCACTGCAGAATTACTCAAAATCGCTAAAACGAATGGAATTGCCATTTTTATCG
TCGGTCATGTTACGAAAGAAGGTTCGATCGCCGGTCCAAGAATGTTAGAGCATATGGTGGATACAGTGTTATATTTTGAA
GGTGAACAACATCATAGTTTCCGAATTTTACGAGCGGTAAAAAACCGATTTGGCTCAACGAACGAGATTGGGATTTTTGA
AATGCATGAGCATGGATTGGAAGAAGTCGCTAACCCTTCACAGATTTTCTTAGAGGAACGCCTAGACGGTGCGACTGGCT
CTGCTATCGTTGTAGCAATGGAAGGAACACGCCCAATTTTGGTAGAGATCCAAGCATTAGTCACACCTACTATGTTCGGA
AATGCTAAGCGAACAACAACAGGTCTTGATTTCAATCGTGTATCTCTAATCATGGCAGTGCTGGAAAAAAGAGCTGGACT
ACTTCTTCAGAATCAAGATGCTTACTTAAAAGCTGCAGGTGGGGTAAAATTAAATGAACCAGCGATTGATTTAGCTATCG
CTGTCAGTATTGCTTCAAGCTACAAAGAAAAAGGAACACAGCCGACGGAATGTTTCATCGGTGAAATCGGACTGACTGGA
GAAATCAGACGTGTCAGTCATATTGAACAACGGGTCAAAGAAGTCCAAAAACTCGGCTTCACAAAAGTTTACCTGCCTAA
AAACAACTTAGGCAACTGGGAAGCGCCAAAAGGAATCGAGATCGTTGGAGTAGCGACTTTGGCTGAAACGTTGAAGCGTG
TGTTCCGCTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

AAA domain (AAA_25)

(70-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Y3JR63

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.286

99.781

0.741
  radA Streptococcus pneumoniae Rx1

74.286

99.781

0.741
  radA Streptococcus pneumoniae D39

74.286

99.781

0.741
  radA Streptococcus pneumoniae R6

74.286

99.781

0.741
  radA Streptococcus pneumoniae TIGR4

74.286

99.781

0.741
  radA Streptococcus mitis SK321

74.066

99.781

0.739
  radA Bacillus subtilis subsp. subtilis str. 168

67.627

98.904

0.669