Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NMK58_RS26915 Genome accession   NZ_CP101292
Coordinates   5211334..5212767 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain 2003-3014     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5206334..5217767
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMK58_RS26885 (NMK58_26885) yrbG 5206892..5207869 (+) 978 WP_000922901.1 calcium/sodium antiporter -
  NMK58_RS26890 (NMK58_26890) kdsD 5207883..5208869 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  NMK58_RS26895 (NMK58_26895) kdsC 5208890..5209456 (+) 567 WP_000030018.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  NMK58_RS26900 (NMK58_26900) lptC 5209453..5210028 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  NMK58_RS26905 (NMK58_26905) lptA 5209997..5210554 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  NMK58_RS26910 (NMK58_26910) lptB 5210561..5211286 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  NMK58_RS26915 (NMK58_26915) rpoN/rpoN1 5211334..5212767 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  NMK58_RS26920 (NMK58_26920) hpf 5212790..5213077 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  NMK58_RS26925 (NMK58_26925) ptsN 5213195..5213686 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NMK58_RS26930 (NMK58_26930) rapZ 5213732..5214586 (+) 855 WP_000243741.1 RNase adapter RapZ -
  NMK58_RS26935 (NMK58_26935) npr 5214583..5214855 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  NMK58_RS26940 (NMK58_26940) yrbL 5215069..5215701 (+) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  NMK58_RS26945 (NMK58_26945) mtgA 5215698..5216426 (-) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  NMK58_RS26950 (NMK58_26950) elbB 5216423..5217076 (-) 654 WP_001299134.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=610142 NMK58_RS26915 WP_000809051.1 5211334..5212767(+) (rpoN/rpoN1) [Escherichia coli strain 2003-3014]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=610142 NMK58_RS26915 WP_000809051.1 5211334..5212767(+) (rpoN/rpoN1) [Escherichia coli strain 2003-3014]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGATGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGATTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACAGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCTCGCAATGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465