Detailed information    

insolico Bioinformatically predicted

Overview

Name   recP/tkt   Type   Machinery gene
Locus tag   NMK58_RS25490 Genome accession   NZ_CP101292
Coordinates   4922739..4924730 (-) Length   663 a.a.
NCBI ID   WP_000098614.1    Uniprot ID   B7UHY1
Organism   Escherichia coli strain 2003-3014     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4917739..4929730
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMK58_RS25465 (NMK58_25465) fumE 4917781..4918290 (-) 510 WP_000224138.1 fumarase E -
  NMK58_RS25470 (NMK58_25470) glpX 4918312..4919277 (-) 966 WP_000987277.1 class II fructose-bisphosphatase -
  NMK58_RS25475 (NMK58_25475) yggP 4919274..4920551 (-) 1278 WP_000853221.1 zinc-binding dehydrogenase -
  NMK58_RS25480 (NMK58_25480) cmtA 4920566..4921954 (-) 1389 WP_000428805.1 PTS mannitol transporter subunit IICB -
  NMK58_RS25485 (NMK58_25485) cmtB 4921982..4922425 (-) 444 WP_001239650.1 PTS mannitol transporter subunit IIA -
  NMK58_RS25490 (NMK58_25490) recP/tkt 4922739..4924730 (-) 1992 WP_000098614.1 transketolase Machinery gene
  NMK58_RS25495 (NMK58_25495) loiP 4925008..4925766 (+) 759 WP_001297457.1 metalloprotease LoiP -
  NMK58_RS25500 (NMK58_25500) - 4925822..4926565 (-) 744 WP_001169551.1 2-dehydro-3-deoxy-phosphogluconate aldolase -
  NMK58_RS25505 (NMK58_25505) dgaE 4926552..4927661 (-) 1110 WP_000193113.1 D-glucosaminate-6-phosphate ammonia lyase -
  NMK58_RS25510 (NMK58_25510) - 4927665..4928525 (-) 861 WP_160333632.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
  NMK58_RS25515 (NMK58_25515) - 4928522..4929271 (-) 750 WP_000380263.1 PTS sugar transporter subunit IIC -

Sequence

Protein


Download         Length: 663 a.a.        Molecular weight: 72211.74 Da        Isoelectric Point: 5.4041

>NTDB_id=610136 NMK58_RS25490 WP_000098614.1 4922739..4924730(-) (recP/tkt) [Escherichia coli strain 2003-3014]
MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDFLKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGY
DLPMEELKNFRQLHSKTPGHPEVGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDGHDAASIKRAVEEARAVTDKPSL
LMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQLGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQ
EAAEFTRRMKGEMPSDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSGSKAINEDA
AGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAALMKQRQVMVYTHDSIGLGEDGPTHQPVEQVA
SLRVTPNMSTWRPCDQVESAVAWKYGVERQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSE
VELAVAAYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVGLNGAIVGMTTFGESAP
AELLFEEFGFTVDNVVAKAKELL

Nucleotide


Download         Length: 1992 bp        

>NTDB_id=610136 NMK58_RS25490 WP_000098614.1 4922739..4924730(-) (recP/tkt) [Escherichia coli strain 2003-3014]
ATGTCCTCACGTAAAGAGCTTGCCAATGCTATTCGTGCGCTGAGCATGGACGCAGTACAGAAAGCCAAATCCGGTCACCC
GGGTGCCCCTATGGGTATGGCTGACATTGCCGAAGTCCTGTGGCGTGATTTCCTGAAACACAACCCGCAGAATCCGTCCT
GGGCTGACCGTGACCGCTTCGTGCTGTCCAACGGCCACGGCTCCATGCTGATCTACAGCCTGCTGCACCTCACCGGTTAC
GATCTGCCGATGGAAGAACTGAAAAACTTCCGTCAGCTGCACTCTAAAACTCCGGGCCACCCGGAAGTGGGTTACACCGC
TGGTGTGGAAACCACCACCGGTCCGCTGGGGCAGGGTATTGCCAACGCAGTAGGTATGGCGATTGCAGAAAAAACGCTGG
CGGCGCAGTTTAACCGTCCGGGCCACGACATTGTCGACCACTACACCTACGCCTTCATGGGCGACGGCTGCATGATGGAA
GGCATCTCCCACGAAGTTTGCTCTCTGGCGGGTACGCTGAAGCTGGGTAAACTGATTGCGTTCTACGATGACAACGGTAT
CTCTATTGATGGTCACGTTGAAGGCTGGTTCACCGACGACACCGCAATGCGTTTCGAAGCTTACGGCTGGCACGTTATTC
GCGACATCGACGGTCATGACGCGGCATCCATCAAACGCGCAGTAGAAGAAGCGCGCGCAGTGACTGACAAACCGTCCCTG
CTGATGTGCAAAACCATCATCGGTTTCGGTTCCCCGAACAAAGCCGGTACTCACGACTCCCACGGTGCGCCGCTGGGCGA
CGCTGAAATTGCCCTGACCCGCGAACAGCTGGGCTGGAAATATGCACCGTTCGAAATCCCGTCTGAAATCTATGCTCAGT
GGGATGCGAAAGAAGCAGGCCAGGCGAAAGAATCCGCATGGAACGAGAAATTCGCTGCTTACGCGAAAGCTTATCCGCAG
GAAGCCGCTGAATTTACCCGCCGTATGAAAGGCGAAATGCCGTCTGACTTCGACGCGAAAGCAAAAGAGTTCATTGCTAA
ACTGCAGGCTAACCCGGCGAAAATCGCCAGCCGTAAAGCGTCTCAGAATGCTATCGAAGCTTTCGGTCCGCTGTTGCCGG
AATTCCTCGGCGGTTCTGCTGACCTGGCACCGTCTAACCTGACCCTGTGGTCTGGTTCTAAAGCAATCAACGAAGATGCT
GCGGGTAACTACATCCACTACGGTGTTCGCGAGTTCGGTATGACCGCGATTGCTAACGGTATCTCCCTGCACGGTGGTTT
CCTGCCGTACACCTCCACCTTCCTGATGTTCGTGGAATACGCACGTAACGCCGTACGTATGGCTGCGCTGATGAAACAGC
GTCAGGTGATGGTTTACACCCACGACTCCATCGGTCTGGGCGAAGATGGCCCGACTCACCAGCCGGTAGAGCAGGTCGCT
TCTCTGCGCGTAACCCCGAACATGTCTACATGGCGTCCGTGTGACCAGGTTGAATCCGCGGTCGCGTGGAAATACGGTGT
TGAGCGTCAGGACGGCCCGACCGCACTGATCCTCTCCCGTCAGAACCTGGCGCAGCAGGAACGAACTGAAGAGCAACTGG
CAAACATCGCGCGCGGTGGTTATGTGCTGAAAGACTGCGCCGGTCAGCCGGAACTGATTTTCATCGCTACCGGTTCAGAA
GTTGAACTGGCTGTTGCTGCCTACGAAAAACTGACTGCCGAAGGCGTGAAAGCGCGCGTGGTGTCCATGCCGTCTACCGA
CGCATTTGACAAGCAGGATGCTGCTTACCGTGAATCCGTACTGCCGAAAGCGGTTACTGCACGCGTTGCTGTAGAAGCGG
GTATTGCTGACTACTGGTACAAGTATGTTGGCCTGAACGGTGCTATCGTCGGTATGACCACCTTCGGTGAATCTGCTCCG
GCAGAGCTGCTGTTTGAAGAGTTCGGCTTCACTGTTGATAACGTTGTTGCGAAAGCAAAAGAACTGCTGTAA

Domains

Predicted by InterProScan.

Transketolase, C-terminal domain (Transketolase_C)

(540-655)

Transketolase, pyrimidine binding domain (Transket_pyr)

(354-524)

Transketolase, thiamine diphosphate binding domain (Transketolase_N)

(4-334)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7UHY1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recP/tkt Streptococcus pneumoniae TIGR4

48.024

99.246

0.477