Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NMT99_RS08570 Genome accession   NZ_CP101290
Coordinates   1646119..1647108 (+) Length   329 a.a.
NCBI ID   WP_003232026.1    Uniprot ID   P51835
Organism   Bacillus subtilis strain LjM2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1641119..1652108
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMT99_RS08555 acpP 1641316..1641549 (+) 234 WP_003154310.1 acyl carrier protein -
  NMT99_RS08560 rncS 1641689..1642438 (+) 750 WP_003232030.1 ribonuclease III -
  NMT99_RS08565 smc 1642539..1646099 (+) 3561 WP_015715874.1 chromosome segregation protein SMC -
  NMT99_RS08570 pilA 1646119..1647108 (+) 990 WP_003232026.1 signal recognition particle-docking protein FtsY Machinery gene
  NMT99_RS08575 sivC 1647145..1647630 (-) 486 WP_003245834.1 Ig-like domain-containing protein -
  NMT99_RS08580 srpM 1647807..1648139 (+) 333 WP_003238590.1 putative DNA-binding protein -
  NMT99_RS08585 ffh 1648153..1649493 (+) 1341 WP_003232020.1 signal recognition particle protein -
  NMT99_RS08590 rpsP 1649599..1649871 (+) 273 WP_003220815.1 30S ribosomal protein S16 -
  NMT99_RS08595 khpA 1649871..1650116 (+) 246 WP_014476792.1 KH domain-containing protein -
  NMT99_RS08600 ylqD 1650238..1650624 (+) 387 WP_014476793.1 YlqD family protein -
  NMT99_RS08605 rimM 1650629..1651153 (+) 525 WP_003232013.1 ribosome maturation factor RimM -
  NMT99_RS08610 trmD 1651150..1651881 (+) 732 WP_003232011.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -

Sequence

Protein


Download         Length: 329 a.a.        Molecular weight: 36342.52 Da        Isoelectric Point: 5.0164

>NTDB_id=609996 NMT99_RS08570 WP_003232026.1 1646119..1647108(+) (pilA) [Bacillus subtilis strain LjM2]
MSFFKKLKEKITKQTDSVSEKFKDGLEKTRNSFQNKVNDLVSRYRKVDEDFFEELEEVLISADVGFTTVMELIDELKKEV
KRRNIQDPKEVQSVISEKLVEIYNSGDEQISELNIQDGRLNVILLVGVNGVGKTTTIGKLAHKMKQEGKSVVLAAGDTFR
AGAIEQLEVWGERTGVPVIKQTAGSDPAAVIYDAVHAAKARNADVLICDTAGRLQNKVNLMKELEKVKRVIEREVPEAPH
EVLLALDATTGQNAMAQAKEFSKATNVTGIALTKLDGTAKGGIVLAIRNELHIPVKLVGLGEKVDDLQEFDPESYVYGLF
SDLVEKADD

Nucleotide


Download         Length: 990 bp        

>NTDB_id=609996 NMT99_RS08570 WP_003232026.1 1646119..1647108(+) (pilA) [Bacillus subtilis strain LjM2]
ATGAGCTTTTTTAAAAAATTAAAAGAGAAAATCACAAAACAGACAGATTCCGTATCTGAAAAGTTTAAGGATGGCCTTGA
AAAAACAAGAAACTCCTTTCAAAACAAAGTGAATGATCTTGTATCCCGTTACCGTAAAGTGGATGAGGATTTCTTCGAAG
AGCTTGAAGAGGTTCTTATCAGCGCGGATGTCGGTTTTACAACCGTTATGGAATTAATAGATGAGCTGAAAAAAGAAGTC
AAACGCAGAAATATTCAAGATCCAAAGGAAGTCCAGTCAGTGATTTCTGAGAAACTGGTCGAGATTTATAACAGCGGAGA
TGAGCAAATTTCAGAACTGAACATCCAGGATGGGCGTTTAAACGTAATCCTTCTGGTAGGTGTAAACGGTGTCGGGAAAA
CAACAACGATCGGAAAGCTTGCTCATAAAATGAAACAAGAAGGAAAATCTGTCGTACTTGCCGCCGGAGACACTTTCAGA
GCGGGAGCCATTGAACAGCTGGAAGTATGGGGAGAGCGTACAGGAGTGCCTGTCATTAAGCAGACGGCCGGAAGCGATCC
GGCAGCTGTCATCTACGATGCTGTTCATGCTGCGAAAGCAAGAAATGCCGATGTATTAATTTGTGATACGGCAGGGCGTC
TCCAAAACAAAGTAAATCTCATGAAAGAGCTTGAAAAAGTAAAACGTGTTATCGAAAGAGAAGTTCCTGAAGCTCCGCAT
GAGGTGCTGCTTGCCCTTGATGCCACGACCGGCCAAAATGCAATGGCTCAGGCAAAAGAATTCTCTAAAGCAACAAATGT
TACCGGCATTGCTTTAACGAAGCTTGACGGTACGGCAAAAGGCGGTATCGTCCTTGCGATTCGCAACGAGCTTCACATCC
CGGTTAAACTAGTCGGTTTAGGAGAAAAAGTTGATGACCTTCAGGAATTTGATCCAGAATCCTATGTGTACGGACTCTTT
TCAGATTTAGTGGAAAAAGCCGACGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(121-320)

SRP54-type protein, helical bundle domain (SRP54_N)

(34-99)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P51835

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

43.709

91.793

0.401