Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   NMT99_RS06225 Genome accession   NZ_CP101290
Coordinates   1207147..1208976 (+) Length   609 a.a.
NCBI ID   WP_003245178.1    Uniprot ID   -
Organism   Bacillus subtilis strain LjM2     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1202147..1213976
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMT99_RS06190 yjbC 1202819..1203397 (+) 579 WP_003224597.1 GNAT family N-acetyltransferase -
  NMT99_RS06195 spx 1203578..1203973 (+) 396 WP_014476435.1 transcriptional regulator Spx -
  NMT99_RS06200 yjbE 1204016..1204672 (-) 657 WP_003232944.1 TerC family protein -
  NMT99_RS06205 - 1204842..1204982 (+) 141 WP_119122854.1 hypothetical protein -
  NMT99_RS06210 mecA 1204949..1205605 (+) 657 WP_003232942.1 adaptor protein MecA Regulator
  NMT99_RS06215 - 1205600..1205722 (-) 123 WP_003232940.1 hypothetical protein -
  NMT99_RS06220 coiA 1205766..1206917 (+) 1152 WP_069703690.1 competence protein CoiA Machinery gene
  NMT99_RS06225 pepF 1207147..1208976 (+) 1830 WP_003245178.1 oligoendopeptidase F Regulator
  NMT99_RS06230 - 1209014..1209181 (-) 168 WP_003244944.1 hypothetical protein -
  NMT99_RS06235 spxH 1209495..1210394 (-) 900 WP_003245184.1 protease adaptor protein SpxH -
  NMT99_RS06240 bhbI 1210391..1210789 (-) 399 WP_003232928.1 group 2 truncated hemoglobin YjbI -
  NMT99_RS06245 cwlQ 1211044..1211778 (-) 735 WP_041340378.1 bifunctional muramidase/murein lytic transglycosylase -
  NMT99_RS06250 yjbK 1211793..1212365 (-) 573 WP_069703692.1 CYTH domain-containing protein -
  NMT99_RS06255 - 1212490..1212858 (+) 369 WP_003232922.1 hypothetical protein -
  NMT99_RS06260 yjbM 1212887..1213522 (+) 636 WP_003245294.1 GTP diphosphokinase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 70085.95 Da        Isoelectric Point: 5.1562

>NTDB_id=609980 NMT99_RS06225 WP_003245178.1 1207147..1208976(+) (pepF) [Bacillus subtilis strain LjM2]
MAEEKKANQLPDRSEVKAEDTWRLEDIFPSDEAWNKEFQAVKELIPNLSKYKGKLADSADHLYEALTYQDKVMERLGRLY
TYAHMRSDQDTGNSFYQGLNDKAGNLYTQAASATAYLVPEILSIEEDKLQQFILEKEELKLYSHAIEEITKERPHVLSEK
EEALLAEASEVLGSSSNTFSVLNNADITFPSIKDEDGNEKQITHGNFINFLESENREVRKNAFDAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKKYKSAREAALSNNSIPEEVYDNLVKTINKHLPLLHRYIALRKKVLELDEVHIYDLYTPLVKDAGM
KVTYEEAKDYMLKGLAPLGEEYASILKEGLENRWVDVYENKGKRNGAYSSGAYGTNPYILMNWHNNVNNLFTLVHEFGHS
VHSYYTRKHQPYPYGNYSIFVAEVASTTNEALLGEYLLNNLEDEKQRLYILNHMLEGFRGTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLTNVYYDLNKKYFGDGMVIDKEIGLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVDRYIDFL
KAGSSQYPIDVLKKAGVDMTSPEPIEAACKMFEEKLDEMEELLMKVKQS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=609980 NMT99_RS06225 WP_003245178.1 1207147..1208976(+) (pepF) [Bacillus subtilis strain LjM2]
ATGGCTGAGGAAAAAAAAGCAAACCAACTGCCTGACAGAAGTGAGGTAAAGGCAGAAGACACATGGAGACTTGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCTGTAAAAGAATTAATTCCGAATTTATCTAAGTATAAAGGAA
AGCTGGCAGATTCAGCTGATCATTTATACGAGGCTCTTACGTATCAAGATAAAGTGATGGAGCGGCTAGGTAGGCTGTAC
ACATATGCGCATATGCGCTCTGACCAGGATACTGGGAACTCCTTTTACCAGGGGCTGAATGACAAGGCGGGAAACCTGTA
TACACAGGCCGCAAGCGCGACAGCTTATTTGGTTCCGGAGATTTTATCCATAGAAGAAGACAAACTGCAGCAGTTCATTC
TTGAAAAAGAAGAATTGAAGCTGTACTCTCATGCGATTGAGGAGATTACAAAGGAACGTCCGCATGTGCTGAGCGAGAAG
GAAGAGGCGCTGCTTGCTGAAGCCTCCGAGGTACTGGGGTCATCTTCAAATACATTCAGCGTGTTAAATAACGCAGATAT
TACGTTTCCGTCCATTAAAGACGAAGATGGGAATGAGAAACAGATCACTCACGGCAACTTTATTAATTTCTTGGAAAGTG
AAAACCGTGAAGTCCGCAAAAATGCGTTTGACGCAGTGTATAAAACGTACGGACAATATAAAAACACAATGGCTACGACG
CTTAGCGGCACTGTGAAAAAGGACAACTTCTACGCGAGAGTGAAAAAATACAAGTCAGCGCGTGAGGCTGCGCTTTCTAA
CAACAGTATTCCTGAGGAAGTATACGATAACCTTGTTAAGACGATTAATAAGCATTTGCCGCTCCTGCACCGCTATATTG
CGCTAAGAAAGAAAGTGCTTGAGCTTGATGAAGTGCATATCTATGACCTGTATACACCGCTTGTGAAAGATGCTGGGATG
AAGGTGACATACGAGGAAGCCAAAGATTACATGCTGAAAGGCCTTGCACCTTTAGGGGAAGAATATGCCTCTATCCTAAA
AGAAGGACTGGAAAACCGCTGGGTGGACGTTTACGAAAATAAAGGCAAACGCAATGGGGCTTATTCATCAGGAGCTTACG
GCACGAATCCGTATATTTTGATGAACTGGCATAATAACGTCAATAATCTCTTTACGCTCGTGCACGAGTTTGGACATTCC
GTACACAGCTACTATACGAGAAAGCACCAGCCTTATCCATATGGCAATTACAGCATCTTTGTCGCGGAAGTTGCCTCTAC
GACAAATGAAGCGCTCCTTGGCGAATATTTGCTGAACAATTTAGAGGATGAAAAACAGCGCTTATATATTCTCAACCATA
TGCTTGAGGGCTTCAGGGGAACGGTTTTCAGACAAACGATGTTCGCTGAATTTGAACATCTGATCCATACAAAGGCGCAA
GAAGGCGAGCCGCTTACACCTGAGCTTCTGACAAATGTCTATTACGACCTGAATAAAAAGTATTTTGGAGACGGCATGGT
GATTGATAAGGAAATCGGCCTTGAATGGTCGCGAATTCCGCACTTCTATTACAATTACTATGTGTATCAGTATGCGACAG
GGTACAGCGCTGCCCAAGCATTAAGCAGCCAGATTTTGAAGGAAGGAAAGCCGGCGGTTGACCGTTATATTGACTTCCTG
AAAGCGGGAAGCTCACAATACCCGATTGATGTCCTGAAAAAAGCGGGTGTTGATATGACGTCTCCAGAGCCAATCGAAGC
CGCGTGCAAAATGTTTGAAGAAAAACTTGATGAAATGGAAGAGCTGCTGATGAAAGTCAAGCAGTCTTAA

Domains

Predicted by InterProScan.

Peptidase family M3 (Peptidase_M3)

(211-590)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

48.822

97.537

0.476