Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NML17_RS03100 Genome accession   NZ_CP101274
Coordinates   659718..660158 (-) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain EC21Z-014     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 654718..665158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NML17_RS03075 (NML17_03075) coaE 655208..655828 (-) 621 WP_001269527.1 dephospho-CoA kinase -
  NML17_RS03080 (NML17_03080) - 655853..655897 (+) 45 WP_120795372.1 protein YacM -
  NML17_RS03085 (NML17_03085) guaC 656053..657096 (+) 1044 WP_001217338.1 GMP reductase -
  NML17_RS03090 (NML17_03090) hofC 657131..658333 (-) 1203 WP_000157234.1 protein transport protein HofC -
  NML17_RS03095 (NML17_03095) pilB 658323..659708 (-) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  NML17_RS03100 (NML17_03100) pilA 659718..660158 (-) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  NML17_RS03105 (NML17_03105) nadC 660361..661254 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NML17_RS03110 (NML17_03110) ampD 661342..661893 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NML17_RS03115 (NML17_03115) ampE 661890..662744 (+) 855 WP_000172006.1 beta-lactamase regulator AmpE -
  NML17_RS03120 (NML17_03120) aroP 662787..664160 (-) 1374 WP_000969914.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=609862 NML17_RS03100 WP_000360895.1 659718..660158(-) (pilA) [Escherichia coli strain EC21Z-014]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=609862 NML17_RS03100 WP_000360895.1 659718..660158(-) (pilA) [Escherichia coli strain EC21Z-014]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363