Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   NML18_RS22685 Genome accession   NZ_CP101269
Coordinates   4675479..4677308 (-) Length   609 a.a.
NCBI ID   WP_001461810.1    Uniprot ID   A7ZU29
Organism   Escherichia coli strain EC21Z-048     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4670479..4682308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NML18_RS22660 (NML18_22660) bioP 4671197..4672096 (-) 900 WP_001196238.1 biotin transporter -
  NML18_RS22665 (NML18_22665) yigL 4672172..4672972 (-) 801 WP_000285352.1 sugar/pyridoxal phosphate phosphatase YigL -
  NML18_RS22670 (NML18_22670) pldB 4672980..4674002 (-) 1023 WP_000487654.1 lysophospholipase L2 -
  NML18_RS22675 (NML18_22675) rhtB 4674113..4674733 (+) 621 WP_000171714.1 homoserine/homoserine lactone efflux protein -
  NML18_RS22680 (NML18_22680) rhtC 4674795..4675415 (-) 621 WP_000928832.1 threonine export protein RhtC -
  NML18_RS22685 (NML18_22685) recQ 4675479..4677308 (-) 1830 WP_001461810.1 ATP-dependent DNA helicase RecQ Machinery gene
  NML18_RS22690 (NML18_22690) pldA 4677441..4678310 (-) 870 WP_001259700.1 phospholipase A -
  NML18_RS22695 (NML18_22695) yigI 4678475..4678942 (+) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  NML18_RS22700 (NML18_22700) rarD 4678994..4679881 (+) 888 WP_000339103.1 EamA family transporter RarD -
  NML18_RS22705 (NML18_22705) - 4679928..4680227 (-) 300 WP_000526434.1 hypothetical protein -
  NML18_RS22710 (NML18_22710) - 4680851..4681003 (-) 153 Protein_4455 CorA family divalent cation transporter -
  NML18_RS22715 (NML18_22715) - 4681196..4682044 (+) 849 WP_001059703.1 YPO3983 family protein -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68337.48 Da        Isoelectric Point: 7.0847

>NTDB_id=609844 NML18_RS22685 WP_001461810.1 4675479..4677308(-) (recQ) [Escherichia coli strain EC21Z-048]
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQINSFDRPNIRYMLMEKFKPLDQLMRYVQEQCGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=609844 NML18_RS22685 WP_001461810.1 4675479..4677308(-) (recQ) [Escherichia coli strain EC21Z-048]
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGAGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGTCGCGATTGCCTGGTCGTCATGCCCACCGGTGGCGGAAAAT
CCCTTTGCTATCAAATTCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAT
CAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAACAGCAACTTGAAGT
GATGACAGGCTGCCGCACCGGGCAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCG
GAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACAC
CACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAACAGTTTTGACCGTCCGAATATTC
GCTACATGCTGATGGAGAAGTTCAAACCGCTCGATCAGTTGATGCGCTACGTGCAGGAACAGTGCGGTAAGTCAGGCATT
ATCTACTGCAACAGCCGCGCGAAAGTAGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGAATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATGTTCGCGCCGATGTGCAGGAAAAATTCCAGCGCGATGACCTGCAAATTGTGGTGGCGACGG
TGGCGTTCGGCATGGGCATCAATAAACCAAACGTTCGCTTCGTGGTCCACTTTGATATTCCGCGCAATATCGAATCCTAT
TATCAGGAAACCGGTCGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCCGATATGGCGTG
GCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAACGTCATAAACTCAATGCGATGGGCGCTT
TTGCCGAAGCGCAAACTTGCCGTCGTCTGGTACTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGTTTTGGTATGGGCTATGTGGTGGAAGTGATTCGCGGCGCTAATAACCAGCGTATCCGCGACTATGGTCATGACA
AACTAAAAGTCTACGGTATGGGCCGTGATAAAAGTCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCTGCGCGTCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATTGCCGATGAAAGCAATGTCCCGCCGTACGTGGTGTTTAACGAC
GCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAA
GCTGGAACGCTTTGGTAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGACGGCGACGACGAAGAGTAG

Domains

Predicted by InterProScan.

RQC domain (RQC)

(406-514)

HRDC domain (HRDC)

(533-599)

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)

DEAD/DEAH box helicase (DEAD)

(28-187)

Helicase conserved C-terminal domain (Helicase_C)

(223-330)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZU29

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.034

97.209

0.389