Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NML24_RS18020 Genome accession   NZ_CP101233
Coordinates   3688620..3689060 (+) Length   146 a.a.
NCBI ID   WP_000360896.1    Uniprot ID   -
Organism   Escherichia coli strain EC21Z-137     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3683620..3694060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NML24_RS18000 (NML24_17975) aroP 3684618..3685991 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  NML24_RS18005 (NML24_17980) ampE 3686034..3686888 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  NML24_RS18010 (NML24_17985) ampD 3686885..3687436 (-) 552 WP_000923727.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NML24_RS18015 (NML24_17990) nadC 3687524..3688417 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NML24_RS18020 (NML24_17995) pilA 3688620..3689060 (+) 441 WP_000360896.1 prepilin peptidase-dependent pilin Machinery gene
  NML24_RS18025 (NML24_18000) pilB 3689070..3690455 (+) 1386 WP_001025156.1 type II secretion system protein GspE Machinery gene
  NML24_RS18030 (NML24_18005) hofC 3690445..3691647 (+) 1203 WP_050010183.1 protein transport protein HofC -
  NML24_RS18035 (NML24_18010) guaC 3691682..3692725 (-) 1044 WP_001217351.1 GMP reductase -
  NML24_RS18040 (NML24_18015) - 3692881..3692925 (-) 45 WP_120795372.1 protein YacM -
  NML24_RS18045 (NML24_18020) coaE 3692950..3693570 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15647.84 Da        Isoelectric Point: 4.3938

>NTDB_id=609356 NML24_RS18020 WP_000360896.1 3688620..3689060(+) (pilA) [Escherichia coli strain EC21Z-137]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQEILNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=609356 NML24_RS18020 WP_000360896.1 3688620..3689060(+) (pilA) [Escherichia coli strain EC21Z-137]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCTGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAATTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363