Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   NML25_RS24260 Genome accession   NZ_CP101222
Coordinates   5052018..5053847 (+) Length   609 a.a.
NCBI ID   WP_024186454.1    Uniprot ID   -
Organism   Escherichia coli strain EC21Z-144     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5047018..5058847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NML25_RS24235 (NML25_24235) - 5047795..5048286 (-) 492 WP_001551977.1 DUF943 family protein -
  NML25_RS24240 (NML25_24240) - 5048283..5049131 (-) 849 WP_001551979.1 YPO3983 family protein -
  NML25_RS24245 (NML25_24245) rarD 5049442..5050332 (-) 891 WP_001551980.1 EamA family transporter RarD -
  NML25_RS24250 (NML25_24250) yigI 5050384..5050851 (-) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  NML25_RS24255 (NML25_24255) pldA 5051016..5051885 (+) 870 WP_001259693.1 phospholipase A -
  NML25_RS24260 (NML25_24260) recQ 5052018..5053847 (+) 1830 WP_024186454.1 ATP-dependent DNA helicase RecQ Machinery gene
  NML25_RS24265 (NML25_24265) rhtC 5053911..5054531 (+) 621 WP_000928844.1 threonine export protein RhtC -
  NML25_RS24270 (NML25_24270) rhtB 5054593..5055213 (-) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  NML25_RS24275 (NML25_24275) pldB 5055324..5056346 (+) 1023 WP_000487654.1 lysophospholipase L2 -
  NML25_RS24280 (NML25_24280) yigL 5056354..5057154 (+) 801 WP_000285352.1 sugar/pyridoxal phosphate phosphatase YigL -
  NML25_RS24285 (NML25_24285) bioP 5057230..5058129 (+) 900 WP_001551984.1 biotin transporter -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68377.53 Da        Isoelectric Point: 7.2544

>NTDB_id=609296 NML25_RS24260 WP_024186454.1 5052018..5053847(+) (recQ) [Escherichia coli strain EC21Z-144]
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGIGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=609296 NML25_RS24260 WP_024186454.1 5052018..5053847(+) (recQ) [Escherichia coli strain EC21Z-144]
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGAGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAGATTATCGACACTGTGCTTTCCGGTCGCGATTGCCTGGTCGTCATGCCCACTGGTGGCGGAAAAT
CCCTTTGCTATCAAATTCCTGCTTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAT
CAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAGCAGCAACTTGAAGT
TATGACAGGCTGTCGCACCGGGCAAATCCGCCTGCTTTATATCGCGCCGGAACGCCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCG
GAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGATGACAC
CACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAGCAGTTTTGACCGTCCGAATATTC
GCTACATGCTGATGGAGAAATTCAAACCGCTCGATCAGTTGATGCGTTATGTACAGGAACAACGCGGTAAGTCGGGGATT
ATTTACTGTAATAGTCGTGCGAAAGTAGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGAATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATGTTCGCGCTGATGTGCAGGAGAAATTCCAGCGCGATGACCTGCAAATTGTGGTGGCGACGG
TGGCGTTCGGCATGGGCATCAATAAACCAAACGTTCGCTTCGTGGTGCATTTTGATATTCCGCGCAACATTGAATCCTAT
TATCAGGAAACCGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCGGATATGGCGTG
GCTGCGTCGTTGTCTGGAAGAGAAACCGCAGGGGCAGTTACAGGATATCGAACGTCATAAACTCAATGCGATGGGCGCGT
TTGCCGAAGCGCAAACCTGTCGCCGTCTGGTGCTGCTAAACTATTTTGGCGAAGGGCGGCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCGAAACAATACGATGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGCGCTAATAACCAGCGTATCCGCGACTATGGTCATGACA
AACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCTGCGCGTCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCACGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCCTTCGGCGGCAACTACGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATTGCCGATGAAAGCAATGTCCCGCCGTACGTGGTGTTTAACGAC
GCGACCTTGATTGAGATGGCGGAACAGATGCCGATCACTGCCAGCGAAATGCTCAGCGTTAACGGCATTGGGATGCGCAA
GCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAG

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(28-187)

HRDC domain (HRDC)

(533-599)

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

RQC domain (RQC)

(406-514)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.034

97.209

0.389