Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NML25_RS22230 Genome accession   NZ_CP101222
Coordinates   4600587..4602083 (-) Length   498 a.a.
NCBI ID   WP_001551783.1    Uniprot ID   -
Organism   Escherichia coli strain EC21Z-144     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4595587..4607083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NML25_RS22205 (NML25_22205) panM 4595809..4596192 (+) 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
  NML25_RS22210 (NML25_22210) livJ 4596392..4597495 (-) 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  NML25_RS22215 (NML25_22215) rpoH 4597766..4598620 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  NML25_RS22220 (NML25_22220) ftsX 4598865..4599923 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  NML25_RS22225 (NML25_22225) ftsE 4599916..4600584 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  NML25_RS22230 (NML25_22230) pilA 4600587..4602083 (-) 1497 WP_001551783.1 signal recognition particle-docking protein FtsY Machinery gene
  NML25_RS22235 (NML25_22235) rsmD 4602233..4602829 (+) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  NML25_RS22240 (NML25_22240) yhhL 4602819..4603088 (+) 270 WP_001295207.1 DUF1145 family protein -
  NML25_RS22245 (NML25_22245) yhhM 4603091..4603450 (-) 360 WP_000042886.1 DUF2500 domain-containing protein -
  NML25_RS22250 (NML25_22250) yhhN 4603591..4604217 (+) 627 WP_000964718.1 lysoplasmalogenase -
  NML25_RS22255 (NML25_22255) zntA 4604291..4606489 (+) 2199 WP_001551784.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  NML25_RS22260 (NML25_22260) tusA 4606591..4606836 (-) 246 WP_000130615.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54701.53 Da        Isoelectric Point: 4.1660

>NTDB_id=609283 NML25_RS22230 WP_001551783.1 4600587..4602083(-) (pilA) [Escherichia coli strain EC21Z-144]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPAKASEQAVEEQPQAHTEAEAETFAAEVVEVTEQVAE
SEKAQPEAEVVAQPELVAEETPEPVAIEREELPLPEDVNAEEVSPEEWRAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=609283 NML25_RS22230 WP_001551783.1 4600587..4602083(-) (pilA) [Escherichia coli strain EC21Z-144]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGCGAAGGCTTCGGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAGGCCGAAGCGGAAACTTTTGCTGCCGAAGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACTGGTCGCAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCGGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCAGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTAGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACTAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATAATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTAGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATTCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

49.864

74.096

0.369